Wound healing in the early chick embryo studied by scanning electron microscopy

1977 ◽  
Vol 152 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Marjorie A. England ◽  
S. Vivienne Cowper
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wound Healing ◽  
Chick Embryo ◽  
Early Chick Embryo ◽  
Scanning Electron

The development of the notochord in the chick embryo, studied by scanning and transmission electron microscopy

Development ◽  
1976 ◽  
Vol 35 (2) ◽  
pp. 383-401
Author(s):  
Mary Bancroft ◽  
Ruth Bellairs
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Chick Embryo ◽  
Intercellular Spaces ◽  
Notochordal Cells ◽  
Fibrillar Material ◽  
Transmission Electron ◽  
Phases Of Development ◽  
Scanning Electron

The notochord of the chick embryo between stages 5 and 23 inclusive has been studied by scanning electron microscopy, supplemented by transmission electron microscopy. Three main phases of development are described, and these have been designated: bilaminar; rodlike, unvacuolated; rod-like and vacuolated. The change in shape of the organ from bilaminar to rod-like is accompanied by changes in the shape, orientation and position of the cells, an increase in the complexity of the cell contacts, and the laying down of a basal lamina. The change from the unvacuolated to the vacuolated phase is accompanied by increasing complexity within the cytoplasm. Most of the vacuoles are intracellular and appear empty though some contain a granular material. The notochordal sheath appears to be secreted by the notochordal cells and fine fibrillar material has been seen in the intercellular spaces. By stage 23, most of the notochordal cells have become so highly vacuolated that the cytoplasm has become closely packed around the nucleus.

scholarly journals The mechanism of somite segmentation in the chick embryo

Development ◽  
1979 ◽  
Vol 51 (1) ◽  
pp. 227-243
Author(s):  
Ruth Bellairs
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Tissue Culture ◽  
Chick Embryo ◽  
Neural Tube ◽  
Cell Shape ◽  
Fibroblast Cell ◽  
General Shape ◽  
Major Factors ◽  
Scanning Electron

The segmentation of somites in the chick embryo has been studied by transmission and scanning electron microscopy (stages 8–14). The segmental plate mesoderm consists of loosely arranged mesenchymal cells, whereas the newly formed somites are composed of elongated, spindle-shaped cells arranged radially around a lumen, the myocoele. The diameter of each somite is thus two cells plus the myocoele. Two major factors appear to be responsible for the change in cell shape at segmentation: (1) Each prospective somite cell becomes anchored at one end to the adjacent epithelia (i.e. the neural tube, the notochord, the ectoderm, the endoderm or the aorta) by means of collagen fibrils. These fibrils are already present in the segmental plate before the somites begin to form. (2) A change in cell-to-cell adhesiveness causes the free ends of these cells to adhere to one another. (Bellairs, Curtis & Sanders, 1978). This adhesion is then supplemented by the development of tight junctions proximally in the somite. Because it is anchored at both ends, each somite cell is under tension in much the same way as a fibroblast cell in tissue culture is under tension. Each somite cell therefore becomes elongated and the somite as a whole accommodates its general shape to that of the space available between the adjacent tissues. The arrangement of the cells in the more differentiated somites (stages 17–18) has also been examined and it has been found that the chick resembles Xenopus in that the myotome cells undergo rotation and become orientated in an anteroposterior direction.

Scanning electron microscopy of wound healing in Xenopus and chicken embryos

Development ◽  
1980 ◽  
Vol 59 (1) ◽  
pp. 341-353
Author(s):  
Martin Stanisstreet ◽  
Jennifer Wakely ◽  
Marjorie A. England
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wound Healing ◽  
Wound Closure ◽  
Primitive Streak ◽  
Major Mechanism ◽  
Xenopus Embryos ◽  
Lateral Tension ◽  
Marginal Cells ◽  
Scanning Electron

Wound closure in the ectoderm of Xenopus early neurulae and chick primitive-streak embryos has been studied by scanning electron microscopy (SEM). Initial gaping of the wound and a cobble-stone appearance of cells peripheral to the wound in both Xenopus and chick confirm that the ectoderm is under lateral tension at these stages. Healing is rapid: in Xenopus embryos wound closure has started within 5 min of wounding; in chick healing is almost complete within 30 min in some cases. The SEM observations suggest that in Xenopus embryos changes in cell shape are the major mechanism for wound closure. In chick embryos wound healing is also accompanied by changes in the shape of the marginal cells, but evidence is presented that in this system cell proliferation is important. The mechanisms of wound healing in Xenopus and chick embryonic ectoderm are compared with those of wound healing in other tissues.

Scanning electron microscopy of the chick embryo

1973 ◽  
Vol 33 (2) ◽  
pp. 457-462 ◽  
Author(s):  
Peter B. Armstrong ◽  
David Parenti
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Chick Embryo ◽  
Scanning Electron

Dermal Neoformation During Skin Wound Healing as Demonstrated Using Scanning Electron Microscopy

2004 ◽  
Vol 52 (4) ◽  
pp. 398-406 ◽  
Author(s):  
Naoto Yamamoto ◽  
Tomoharu Kiyosawa ◽  
Katsuyuki Arai ◽  
Yoshio Nakayama
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wound Healing ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Scanning Electron

Complete transposition in a chick embryo demonstrated by scanning electron microscopy

1998 ◽  
Vol 8 (3) ◽  
pp. 396-399 ◽  
Author(s):  
Jörg Männer ◽  
Wolfgang seidl ◽  
Gerd Steding
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Animal Models ◽  
Chick Embryo ◽  
Chick Embryos ◽  
Documented Case ◽  
The Past ◽  
Complete Transposition ◽  
Developing Heart ◽  
Scanning Electron

AbstractChick embryos are frequently used as animal models when researching the developing heart. In the past, every attempt to induce complete transposition (the combination of concordant arrioventricular and discordant ventriculo-arterial connections) failed in chicks, suggesting that it might be impossible to develop a chicken modle for this malformation. We demonstrate, to the best of our knowledge, the first well-documented case of complete transpositon occourrine in the chick

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