In vitro studies on the embryotoxic potential of (bis[tri-n-butyltin])oxide in a limb bud organ culture system

1986 ◽  
Vol 58 (3) ◽  
pp. 125-129 ◽  
Author(s):  
Ralf Krowke ◽  
Ursula Bluth ◽  
Diether Neubert
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
In Vitro Studies ◽  
Limb Bud ◽  
Organ Culture System

scholarly journals Hst-1 (FGF-4) antisense oligonucleotides block murine limb development.

1995 ◽  
Vol 130 (4) ◽  
pp. 997-1003 ◽  
Author(s):  
T Ochiya ◽  
H Sakamoto ◽  
M Tsukamoto ◽  
T Sugimura ◽  
M Terada
Keyword(s):  
Organ Culture ◽  
Limb Development ◽  
Culture System ◽  
Inhibitory Effect ◽  
Defined Medium ◽  
Limb Bud ◽  
Embryonic Mouse ◽  
Organ Culture System ◽  
Mouse Limb

The initiation of limb development depends on the site specific proliferation of the mesenchyme by the signals from the apical ectodermal ridge (AER) in embryonic mouse. We have previously reported that the local expression of Hst-1/Fgf-4 transcripts in AER of the mouse limb bud is developmentally regulated, expressed at 11 and 12 days post coitus (p.c.) embryo. In an effort to further understand the role of Hst-1/FGF-4 in mouse limb development, an antisense oligodeoxynucleotides (ODNs) study was performed. We first established a novel organ culture system to study mouse limb development in vitro. This system allows mouse limb bud at 9.5-10-d p.c. embryo, when placed on a sheet of extracellular matrix in a defined medium, to differentiate into a limb at 12.5-d p.c. embryo within 4.5 d. Using this organ culture system, we have shown that exposure of 9.5-10-d p.c. embryonal limb bud explants to antisense ODNs of Hst-1/FGF-4 blocks limb development. In contrast, sense and scrambled ODNs have no inhibitory effect on limb outgrowth, suggesting that Hst-1/FGF-4 may work as a potent inducing factor for mouse limb development.

In vivo and in vitro Study of the Effects of Vitamin A Deficiency on Rat Third Molar Development

1986 ◽  
Vol 65 (12) ◽  
pp. 1445-1448 ◽  
Author(s):  
S.S. Harris ◽  
J.M. Navia
Keyword(s):  
Vitamin A ◽  
Organ Culture ◽  
Culture System ◽  
Vitamin A Deficiency ◽  
Third Molar ◽  
In Vitro Study ◽  
Organ Culture System ◽  
Vitamin A Status

We have examined the effect of in vivo vitamin A status on subsequent rat third molar formation and mineralization in an in vitro organ culture system. Vitamin A deficiency imposed during an eight-day in vitro period caused effects very similar to those of vitamin A deficiency imposed on rats in vivo. Analysis of the data also demonstrates that retinoic acid is capable of reversing the interference in mineralization of third molars induced by vitamin A deficiency in the organ culture system.

Usefulness of the organ culture system in the in vitro diagnosis of Celiac Disease: A multicenter study

2006 ◽  
Vol 38 ◽  
pp. S80-S81
Author(s):  
A. Picarelli ◽  
M. Di Tola ◽  
L. Sabbatella ◽  
M.C. Anania ◽  
A. Calabrò ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Organ Culture ◽  
Multicenter Study ◽  
Culture System ◽  
Organ Culture System ◽  
In Vitro Diagnosis

Growth hormone and somatostatin directly inhibit gastric ghrelin secretion. An in vitro organ culture system

2007 ◽  
Vol 30 (9) ◽  
pp. RC22-RC25 ◽  
Author(s):  
L. M. Seoane ◽  
O. Al-Massadi ◽  
F. Barreiro ◽  
C. Dieguez ◽  
F.F Casanueva
Keyword(s):  
Growth Hormone ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System ◽  
In Vitro Organ Culture ◽  
Ghrelin Secretion

scholarly journals Phenotypic Mapping of The Chicken Embryonic Thymic Microenvironment Developing Within an Organ Culture System

1993 ◽  
Vol 3 (2) ◽  
pp. 147-158 ◽  
Author(s):  
Natalie J. Davidson ◽  
Richard L. Boyd
Keyword(s):  
Organ Culture ◽  
Mhc Class Ii ◽  
Culture System ◽  
Antigen Expression ◽  
Cell Types ◽  
Normal Embryo ◽  
Organ Culture System ◽  
Stromal Component ◽  
Epithelial Antigens

The chicken thymic microenvironment, as it developed in an embryonic thymus organ culture system, was phenotypically mapped using a panel of mAb defining both epithelial and nonepithelial stromal cell antigens. We have previously reported that thymocyte proliferation and differentiation will proceed for up to 6–8 days in thymus organ culture, hence demonstrating the functional integrity of the thymic microenvironmentin vitro. During this time, the stromal component reflected that of the normal embryo with cortical and medullary epithelial areas readily identifiable by both morphology and surface-antigen expression. An abundance of subcapsular and cortical epithelial antigens was detected in the cultured thymus, particularly those normally expressed by the epithelium lining the capsule, trabeculae, and vascular regions (type epithelium) in the adult and embryonic thymus. Medullary epithelial antigens developed in organ culture, although were present in lower frequency than observed in the age-matched embryonic thymus. MHC class II expression by both epithelial and nonepithelial cells was maintained at high levels throughout the culture period. With increasing time in culture, the ratio of epithelial to nonepithelial cells decreased, concurrent with a decrease in thymocyte frequency and suggestive of a bidirectional interaction between these two cell types. Thus, a functionally intact thymic microenvironment appears to be maintained in embryonic thymus organ culture, a model that is currently being exploited to assess the role of stromal antigens, as defined by our mAb, in the process of thymopoiesis.

THE EFFECT OF OESTROGENS ON THE RESPONSE OF BONE TO PARATHYROID HORMONE IN VITRO

1972 ◽  
Vol 54 (1) ◽  
pp. 107-117 ◽  
Author(s):  
D. ATKINS ◽  
JOAN M. ZANELLI ◽  
M. PEACOCK ◽  
B. E. C. NORDIN
Keyword(s):  
Parathyroid Hormone ◽  
Citric Acid ◽  
Organ Culture ◽  
Culture System ◽  
Glucose Consumption ◽  
Organ Culture System ◽  
Mouse Calvaria ◽  
Ethinyl Oestradiol

SUMMARY An organ culture system was used to examine the effects of oestrogens on the response of 5-day-old mouse calvaria to parathyroid hormone (PTH). Parathyroid hormone released calcium and phosphate from the bone and this was associated with an increase in glucose consumption, an accumulation of citric acid and an inhibition of citrate oxidation. Oestradiol, oestriol, oestrone and ethinyl oestradiol all inhibited the PTH-induced release of calcium. The accumulation of citrate was prevented without the PTH-induced block on citrate oxidation being removed, and this was explained in terms of a reduction in glycolysis. Oestradiol, oestriol and oestrone appeared to be of equal potency. However, ethinyl oestradiol was active at much lower doses but appeared to be toxic at higher levels.

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