Regulation of plasmid DNA synthesis: Isolation and characterization of copy number mutants of miniR6-5 and miniF plasmids

1981 ◽  
Vol 181 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Susan Ely ◽  
Walter L. Staudenbauer
Keyword(s):  
Dna Synthesis ◽  
Plasmid Dna ◽  
Copy Number ◽  
Isolation And Characterization

Isolation and characterization of the Schizosaccharomyces pombe rad3 gene, involved in the DNA damage and DNA synthesis checkpoints

Gene ◽  
1992 ◽  
Vol 119 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Brent L. Seaton ◽  
Jennifer Yucel ◽  
Per Sunnerhagen ◽  
Suresh Subramani
Keyword(s):  
Dna Damage ◽  
Dna Synthesis ◽  
Schizosaccharomyces Pombe ◽  
Isolation And Characterization

Isolation and characterization of a plasmid DNA from periodontopathogenic bacterium, Eikenella corrodens 1073, which affects pilus formation and colony morphology

Gene ◽  
2005 ◽  
Vol 351 ◽  
pp. 143-148 ◽  
Author(s):  
Hiroyuki Azakami ◽  
Hiromi Akimichi ◽  
Masakatsu Usui ◽  
Hiromichi Yumoto ◽  
Shigeyuki Ebisu ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Colony Morphology ◽  
Eikenella Corrodens ◽  
Isolation And Characterization

scholarly journals ISOLATION AND CHARACTERIZATION OF dnaX AND dnaY TEMPERATURE-SENSITIVE MUTANTS OF ESCHERICHIA COLI

Genetics ◽  
1979 ◽  
Vol 92 (4) ◽  
pp. 1041-1059
Author(s):  
Joan M Henson ◽  
Herman Chu ◽  
Carleen A Irwin ◽  
James R Walker
Keyword(s):  
Escherichia Coli ◽  
Dna Synthesis ◽  
Fold Increase ◽  
Temperature Sensitive ◽  
E Coli ◽  
Temperature Sensitive Mutants ◽  
Isolation And Characterization ◽  
Ts Mutations

ABSTRACT Escherichia coli mutants with temperature-sensitive (ts) mutations in dnaX and dnaY genes have been isolated. Based on transduction by phage PI, dnaX and Y have been mapped at minutes 10.4-10.5 and 12.1, respectively, in the sequence dnaX purE dnaY. Both dnaXts36 and YtslO are recessive to wild-type alleles present on episomes. F13 carries both dnaX  + and Y  +; the shorter F210 carries dnaY  +, but not X  +. Lambda transducing phages that carry dnaX  + or Y  + have been isolated, and hybrid plasmids of Col E1 and E. coli DNA from the CLARKE and CARBON (1976) collection also carry portions of the dnaX purE dnaY region. Results obtained with the λ transducing phages and the hybrid plasmids suggest that dnaX is a different gene from the previously characterized dnaZ gene, which is also near minute 10.5.—The dnaXts36 mutant, after a shift to 42°, stopped DNA synthesis gradually, and the total amount of DNA increased two-fold. When this mutant was shifted to M°, the rate of DNA synthesis dropped immediately and the final increment of DNA was only 10% of the initial amount. Replicative DNA synthesis in toluene-treated cells was completely inhibited at 42° and was partially in-hibited even at 30°.—When the dnaYtslO mutant was shifted to 42°, DNA synthesis gradually stopped, and the amount of DNA increased 3.6-fold. At 44°, residual DNA synthesis amounted to a two-fold increase. Replicative DNA synthesis in vitro in toluene-treated cells was inactivated after 20 minutes at 42° or by "preincubation" of cells at 42° before toluene treatment.— The dnaX and dnaY products probably function in polymerization of DNA, although participation also in initiation cannot be excluded.

Isolation and Plasmid Characterization of a Lactobacillus Species Involved in the Manufacture of Fermented Sausage1

1985 ◽  
Vol 48 (12) ◽  
pp. 1028-1035 ◽  
Author(s):  
DENNIS A. ROMERO ◽  
LARRY L. McKAY
Keyword(s):  
Lactic Acid ◽  
Polyethylene Glycol ◽  
Plasmid Dna ◽  
Acid Production ◽  
Gas Production ◽  
Lactobacillus Species ◽  
Erythromycin Resistance ◽  
Streptococcus Lactis ◽  
Isolation And Characterization

Isolation and characterization of a Lactobacillus species capable of proper acid production in a sausage environment is described. The isolate from sausage, categorized as a lactobacillus in the subgenus Streptobacterium, was designated Lactobacillus sp. DR1. Growth occurred at 5 and 42°C but not at 45°C. Fructose, galactose, glucose, mannose, melibiose, N-acetylglucosamine, ribose, sucrose and trehalose were fermented. Gas production from glucose was not observed. In MRS glucose broth, D(−) and L(+) lactic acid were produced. Lactobacillus sp. DR1 contained a single cryptic plasmid of approximately 30 megadaltons (Mdal). In sausage fermentation trials, both Lactobacillus sp. DR1 and plasmid-free derivative DR1C lowered the pH to below 5.3 after 8 h in the smokehouse. Conjugation was demonstrated through the transfer of plasmid pAMβ1, which encodes erythromycin resistance, from Streptococcus lactis 2301β to Lactobacillus sp. DR1. Mutanolysin-generated protoplasts could be regenerated using 0.5 M ammonium chloride, lactose, maltose or sucrose as osmotic stabilizers. Regeneration frequencies ranged from less than 1.0% up to 35%; however, transformation of Lactobacillus sp. DR1 protoplasts by plasmid DNA in the presence of polyethylene glycol (PEG) was unsuccessful.

scholarly journals Isolation and Characterization of Plasmid DNA in Lactobacillus acidophilus.

1991 ◽  
Vol 55 (8) ◽  
pp. 2051-2056 ◽  
Author(s):  
Kazuo KANATANI ◽  
Kazushi YOSHIDA ◽  
Takatsugu TAHARA ◽  
Hirosumi MIURA ◽  
Masaru SAKAMOTO ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Lactobacillus Acidophilus ◽  
Isolation And Characterization

Isolation and characterization of plasmid DNA fromRuminococcus

1987 ◽  
Vol 16 (2) ◽  
pp. 97-100 ◽  
Author(s):  
Roderick V. Asmundson ◽  
William J. Kelly
Keyword(s):  
Plasmid Dna ◽  
Isolation And Characterization

Isolation and characterization of new copy mutants of plasmid R1, and identification of a polypeptide involved in copy number control

1981 ◽  
Vol 181 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Søren Molin ◽  
Peter Stougaard ◽  
Janice Light ◽  
Marie Nordström ◽  
Kurt Nordström
Keyword(s):  
Copy Number ◽  
Copy Number Control ◽  
Isolation And Characterization ◽  
Plasmid R1
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