Cloning of the glnA, ntrB and ntrC genes of Klebsiella pneumoniae and studies of their role in regulation of the nitrogen fixation (nif) gene cluster

1982 ◽  
Vol 186 (4) ◽  
pp. 518-524 ◽  
Author(s):  
Guadalupe Espin ◽  
Ariel Alvarez-Morales ◽  
Frank Cannon ◽  
Ray Dixon ◽  
Mike Merrick
Keyword(s):  
Nitrogen Fixation ◽  
Klebsiella Pneumoniae ◽  
Gene Cluster ◽  
Nif Gene ◽  
Nif Gene Cluster

Analysis of regulation of Klebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusions

Nature ◽  
1980 ◽  
Vol 286 (5769) ◽  
pp. 128-132 ◽  
Author(s):  
Ray Dixon ◽  
Robert R. Eady ◽  
Guadalupe Espin ◽  
Susan Hill ◽  
Maurizio Iaccarino ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Klebsiella Pneumoniae ◽  
Gene Cluster ◽  
Gene Fusions ◽  
Nif Gene ◽  
Nif Gene Cluster

Polarity of mutations induced by insertion of transposons Tn5, Tn7 and Tn10 into the nif gene cluster of Klebsiella pneumoniae

1978 ◽  
Vol 165 (1) ◽  
pp. 103-111 ◽  
Author(s):  
Mike Merrick ◽  
Mechthild Filser ◽  
Christina Kennedy ◽  
Ray Dixon
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gene Cluster ◽  
Nif Gene ◽  
Nif Gene Cluster

scholarly journals Evolution and Functional Analysis of orf1 Within nif Gene Cluster from Paenibacillus graminis RSA19

2019 ◽  
Vol 20 (5) ◽  
pp. 1145 ◽  
Author(s):  
Qin Li ◽  
Xiaomeng Liu ◽  
Haowei Zhang ◽  
Sanfeng Chen
Keyword(s):  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Gene Clusters ◽  
Amino Acid Sequences ◽  
Transcriptional Analysis ◽  
Facultative Anaerobic ◽  
Orf1 Gene ◽  
Nif Gene ◽  
Nif Gene Cluster ◽  
Paenibacillus Species

Paenibacillus is a genus of Gram-positive, facultative anaerobic and endospore-forming bacteria. Genomic sequence analysis has revealed that a compact nif (nitrogen fixation) gene cluster comprising 9–10 genes nifBHDKENX(orf1)hesAnifV is conserved in diazotrophic Paenibacillus species. The evolution and function of the orf1 gene within the nif gene cluster of Paenibacillus species is unknown. In this study, a careful comparison analysis of the compositions of the nif gene clusters from various diazotrophs revealed that orf1 located downstream of nifENX was identified in anaerobic Clostridium ultunense, the facultative anaerobic Paenibacillus species and aerobic diazotrophs (e.g., Azotobacter vinelandii and Azospirillum brasilense). The predicted amino acid sequences encoded by the orf1 gene, part of the nif gene cluster nifBHDKENXorf1hesAnifV in Paenibacillus graminis RSA19, showed 60–90% identity with those of the orf1 genes located downstream of nifENX from different diazotrophic Paenibacillus species, but shared no significant identity with those of the orf1 genes from different taxa of diazotrophic organisms. Transcriptional analysis showed that the orf1 gene was expressed under nitrogen fixation conditions from the promoter located upstream from nifB. Mutational analysis suggested that the orf1 gene functions in nitrogen fixation in the presence of a high concentration of O2.

Cloning of a nitrogen fixation (nif) gene cluster of Azospirillum brasilense

Biochimie ◽  
1982 ◽  
Vol 64 (7) ◽  
pp. 495-502 ◽  
Author(s):  
Bernadette Quiviger ◽  
Claudine Franche ◽  
Georges Lutfalla ◽  
Douglas Rice ◽  
Robert Haselkorn ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Azospirillum Brasilense ◽  
Nif Gene ◽  
Nif Gene Cluster
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