Transfer of incP plasmids into Stigmatella aurantiaca leading to insertional mutants affected in spore development

1988 ◽  
Vol 214 (2) ◽  
pp. 213-217 ◽  
Author(s):  
Ingrid Glomp ◽  
Patrick Saulnier ◽  
Janine Guespin-Michel ◽  
Hans Ulrich Schairer
Keyword(s):  
Spore Development ◽  
Insertional Mutants ◽  
Incp Plasmids ◽  
Stigmatella Aurantiaca

Exemplar Abstract for Stigmatella aurantiaca Berkeley 1875 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Stigmatella Aurantiaca

EU-OSTID: A Collection of Transposon Insertional Mutants for Functional Genomics in Rice

2005 ◽  
Vol 59 (1) ◽  
pp. 99-110 ◽  
Author(s):  
L. J. G van Enckevort ◽  
Gaëtan Droc ◽  
Pietro Piffanelli ◽  
Raffaella Greco ◽  
Cyril Gagneur ◽  
...  
Keyword(s):  
Functional Genomics ◽  
Insertional Mutants

scholarly journals Genetic analysis of SecA–SecY interaction required for spore development inBacillus subtilis

2000 ◽  
Vol 184 (2) ◽  
pp. 285-289
Author(s):  
Hitomi Kobayashi ◽  
Yoshiaki Ohashi ◽  
Hideaki Nanamiya ◽  
Kei Asai ◽  
Fujio Kawamura
Keyword(s):  
Genetic Analysis ◽  
Spore Development

Identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic Antarctic ciliate Euplotes focardii

2014 ◽  
Vol 26 (5) ◽  
pp. 491-501 ◽  
Author(s):  
Sandra Pucciarelli ◽  
Federica Chiappori ◽  
Raghul Rajan Devaraj ◽  
Guang Yang ◽  
Ting Yu ◽  
...  
Keyword(s):  
Amino Acid Level ◽  
Bacterial Symbiont ◽  
The Arctic ◽  
Glacial Ice ◽  
Mould Fungus ◽  
Lake Vostok ◽  
Ice Binding ◽  
Stigmatella Aurantiaca ◽  
Ice Binding Protein ◽  
The Antarctic

AbstractWe identified two ice-binding protein (IBP) sequences, named EFsymbAFP and EFsymbIBP, from a putative bacterial symbiont of the Antarctic psychrophilic ciliate Euplotes focardii. EFsymbAFP is 57.43% identical to the antifreeze protein (AFP) from the Stigmatella aurantiaca strain DW4/3-1, which was isolated from the Victoria Valley lower glacier. EFsymbIBP is 53.38% identical to the IBP from the Flavobacteriaceae bacterium strain 3519-10, isolated from the glacial ice of Lake Vostok. EFsymbAFP and EFsymbIBP are 31.73% identical at the amino acid level and are organized in tandem on the bacterial chromosome. The relatively low sequence identity and the tandem organization, which appears unique to this symbiont, suggest an occurrence of horizontal gene transfer (HGT). Structurally, EFsymbAFP and EFsymbIBP are similar to the AFPs from the snow mould fungus Typhula ishikariensis and from the Arctic yeast Leucosporidium sp. AY30. A phylogenetic analysis showed that EFsymbAFP and EFsymbIBP cluster principally with the IBP sequences from other Antarctic bacteria, supporting the view that these sequences belong to an Antarctic symbiontic bacterium of E. focardii. These results confirm that IBPs have a complex evolutionary history, which includes HGT events, most probably due to the demands of the environment and the need for rapid adaptation.

Dynamic localization of penicillin-binding proteins during spore development in Bacillus subtilis

Microbiology ◽  
2005 ◽  
Vol 151 (3) ◽  
pp. 999-1012 ◽  
Author(s):  
Dirk-Jan Scheffers
Keyword(s):  
Bacillus Subtilis ◽  
Green Fluorescent Protein ◽  
Binding Proteins ◽  
Cytoplasmic Membrane ◽  
Fluorescent Protein ◽  
Spore Formation ◽  
The Other ◽  
Spore Development ◽  
Penicillin Binding Proteins ◽  
Green Fluorescent

During Bacillus subtilis spore formation, many membrane proteins that function in spore development localize to the prespore septum and, subsequently, to the outer prespore membrane. Recently, it was shown that the cell-division-specific penicillin-binding proteins (PBPs) 1 and 2b localize to the asymmetric prespore septum. Here, the author studied the localization of other PBPs, fused to green fluorescent protein (GFP), during spore formation. Fusions to PBPs 4, 2c, 2d, 2a, 3, H, 4b, 5, 4a, 4* and X were expressed during vegetative growth, and their localization was monitored during sporulation. Of these PBPs, 2c, 2d, 4b and 4* have been implicated as having a function in sporulation. It was found that PBP2c, 2d and X changed their localization, while the other PBPs tested were not affected. The putative endopeptidase PbpX appears to spiral out in a pattern that resembles FtsZ redistribution during sporulation, but a pbpX knockout strain had no distinguishable phenotype. PBP2c and 2d localize to the prespore septum and follow the membrane during engulfment, and so are redistributed to the prespore membrane. A similar pattern was observed when GFP–PBP2c was expressed in the mother cell from a sporulation-specific promoter. This work shows that various PBPs known to function during sporulation are redistributed from the cytoplasmic membrane to the prespore.

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