Use of the gef gene in a biological containment system in soil microcosms

1991 ◽  
Vol 11 (4) ◽  
pp. 301-305 ◽  
Author(s):  
R. B. Henschke ◽  
E. J. Henschke ◽  
F. R. J. Schmidt
Keyword(s):  
Soil Microcosms ◽  
Biological Containment ◽  
Containment System

Plant-dependent active biological containment system for recombinant rhizobacteria

2003 ◽  
Vol 6 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Pieter Van Dillewijn ◽  
Susana Vílchez ◽  
José A. Paz ◽  
Juan L. Ramos
Keyword(s):  
Biological Containment ◽  
Containment System

scholarly journals An engineered bacterium auxotrophic for an unnatural amino acid: a novel biological containment system

2015 ◽  
Author(s):  
Yusuke Kato
Keyword(s):  
Amino Acid ◽  
Natural Environment ◽  
Culture Medium ◽  
Stop Codon ◽  
Unnatural Amino Acid ◽  
Biological Containment ◽  
Containment System ◽  
Genetic Technique ◽  
Colicin E3 ◽  
Host Killing

Biological containment is a genetic technique to program dangerous organisms to grow only in the laboratory and to die in the natural environment. Auxotropy for a substance not found in the natural environment is an ideal biological containment. Here, we constructed an Escherichia coli strain that cannot survive in the absence of the unnatural amino acid 3-iodo-L-tyrosine. This synthetic auxotrophy was achieved by conditional production of the antidote protein against the highly toxic enzyme colicin E3. An amber stop codon was inserted in the antidote gene. The translation of the antidote mRNA was controlled by a translational switch using amber-specific 3-iodo-L-tyrosine incorporation. The antidote is synthesized only when 3-iodo-L-tyrosine is present in the culture medium. The viability of this strain rapidly decreased with less than a 1 h half-life after removal of 3-iodo-L-tyrosine, suggesting that the decay of the antidote causes the host killing by activated colicin E3 in the absence of this unnatural amino acid. This containment system can be constructed by only plasmid introduction without genome editing, suggesting that this system may be applicable to other microbes carrying toxin-antidote systems similar to that of colicin E3.

scholarly journals A biological containment system for Rhizobium meliloti based on the use of recombination-deficient (recA−) strains

1995 ◽  
Vol 16 (3) ◽  
pp. 223-232 ◽  
Author(s):  
Werner Selbitschka ◽  
Uwe Dresing ◽  
Margit Hagen ◽  
Stefan Niemann ◽  
Alfred Pühler
Keyword(s):  
Rhizobium Meliloti ◽  
Biological Containment ◽  
Containment System

scholarly journals An engineered bacterium auxotrophic for an unnatural amino acid: a novel biological containment system

2015 ◽  
Author(s):  
Yusuke Kato
Keyword(s):  
Amino Acid ◽  
Natural Environment ◽  
Culture Medium ◽  
Stop Codon ◽  
Unnatural Amino Acid ◽  
Biological Containment ◽  
Containment System ◽  
Genetic Technique ◽  
Colicin E3 ◽  
Host Killing

Biological containment is a genetic technique to program dangerous organisms to grow only in the laboratory and to die in the natural environment. Auxotropy for a substance not found in the natural environment is an ideal biological containment. Here, we constructed an Escherichia coli strain that cannot survive in the absence of the unnatural amino acid 3-iodo-L-tyrosine. This synthetic auxotrophy was achieved by conditional production of the antidote protein against the highly toxic enzyme colicin E3. An amber stop codon was inserted in the antidote gene. The translation of the antidote mRNA was controlled by a translational switch using amber-specific 3-iodo-L-tyrosine incorporation. The antidote is synthesized only when 3-iodo-L-tyrosine is present in the culture medium. The viability of this strain rapidly decreased with less than a 1 h half-life after removal of 3-iodo-L-tyrosine, suggesting that the decay of the antidote causes the host killing by activated colicin E3 in the absence of this unnatural amino acid. This containment system can be constructed by only plasmid introduction without genome editing, suggesting that this system may be applicable to other microbes carrying toxin-antidote systems similar to that of colicin E3.

Evaluation of the E. coli ribosomal rrnB P1 promoter and phage-derived lysis genes for the use in a biological containment system: A concept study

1995 ◽  
Vol 39 (2) ◽  
pp. 137-148 ◽  
Author(s):  
Karsten Tedin ◽  
Angela Witte ◽  
Günter Reisinger ◽  
Werner Lubitz ◽  
Udo Bläsi
Keyword(s):  
E Coli ◽  
Concept Study ◽  
Biological Containment ◽  
System A ◽  
Containment System

Chemical stabilization of toxic metals in soil microcosms

2009 ◽  
Vol 17 (3) ◽  
pp. 483-494 ◽  
Author(s):  
Viktoria Feigl ◽  
Nikolett Uzinger ◽  
Katalin Gruiz
Keyword(s):  
Toxic Metals ◽  
Chemical Stabilization ◽  
Soil Microcosms
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