Outcross-dependent transpositions of copia-like mobile genetic elements in chromosomes of an inbred Drosophila melanogaster stock

1988 ◽  
Vol 212 (2) ◽  
pp. 281-286 ◽  
Author(s):  
E. G. Pasyukova ◽  
E. S. Belyaeva ◽  
L. E. Ilyinskaya ◽  
V. A. Gvozdev
Keyword(s):  
Drosophila Melanogaster ◽  
Mobile Genetic Elements ◽  
Genetic Elements

Mobile genetic elements in Drosophila melanogaster (recent experiments)

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 920-928 ◽  
Author(s):  
Georgii P. Georgiev ◽  
Nickolai A. Tchurikov ◽  
Yurii V. Ilyin ◽  
Sofia G. Georgieva ◽  
Lev J. Mizrokhi ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Rna Polymerase Ii ◽  
Mobile Element ◽  
Complete Removal ◽  
Mobile Genetic Elements ◽  
Internal Promoter ◽  
Genetic Elements ◽  
First Case ◽  
Large Numbers ◽  
White Locus

Recent data obtained in the authors' laboratories concerning the behaviour of mobile genetic elements of Drosophila melanogaster are reviewed. It was found that the mobile element jockey represents the typical LINE element. It is efficiently transcribed in D. melanogaster cells in flies and in culture. Transcription is initiated from the +1 nucleotide of jockey and depends on an internal promoter. This is the first case of an internal promoter being used by RNA polymerase II. Several events which take place during the transposition bursts in ctMR2 family of strains were described. Among them are the removal of mobile dispersed genetics (mdg) elements (with solo long terminal repeat (LTR) remaining at the site of excision), complete removal of an mdg element, and reinsertion of the same mdg to the same place either in the presence or in absence of solo LTR sequence. Finally, the formation of deletions was observed. A 462-bp deletion destroying the white locus can be further repaired (w+ reversion). Thus, transposition bursts include many different genetic events. A novel system of prolonged genome destabilization was described. It depends on mobilization of a new mobile element called Stalker. After certain crosses Stalker actively moves for dozens of generations giving rise to large numbers of insertion mutations. Several novel genes were detected using mobilized Stalker. They include a modifier of mdg4 and six enhancers of yellow mutations.Key words: Drosophila melanogaster, mobile elements, transcription.

scholarly journals Changes in the intralinear level of hybrid dys-genesis manifestation in Drosophila melanogaster in crosses with individuals with tetracycline suppressed internal microflora

2019 ◽  
Vol 25 ◽  
pp. 38-43
Author(s):  
I. D. Gorodnyanski
Keyword(s):  
Drosophila Melanogaster ◽  
Mobile Genetic Elements ◽  
The Body ◽  
Hybrid Dysgenesis ◽  
Control Line ◽  
Specific Interactions ◽  
Wolbachia Pipientis ◽  
Reciprocal Crosses ◽  
Genetic Elements ◽  
Reciprocal Crossing

Aim. To assess the frequency of hybrid dysgenesis in the offspring of reciprocal crosses of individuals of the Oregon-R line, with individuals of the same line, from the organism of which Wolbachia pipientis was eliminated. Methods. The line of Drosophila melanogaster Oregon R (Cambridge), in the genome of which there are MGE hobo, was investiga-ted. This line is infected with wolbachia of the wMel strain. Removal of Wolbachia from the body was implemented using antibiotic tetracycline. The study used the fifth of the generations obtained on a normal handling medium after the removal of Wolbachia. The level of hybrid dysgenesis was studied by the method of extracting gonad flies and assessing their condition. Results. In the progeny from experimental crossing of females from which Wolbachia was eli-minated, with males of the control line, the proportion of females with fully reduced gonads is significantly higher than in the offspring of reciprocal crossing. Conclusions. The results obtained may be a sign of the action of the system of specific interactions of Wolbachia pipientis and Drosophila’s MGE. Keywords: mobile genetic elements, hybrid dysgenesis, Drosophila melanogaster, Wolbachia pipientis.

scholarly journals Effect of suppression of internal microflora by tetracycline on the level of manifestation of the hybrid dysgenesis of Drosophila melanogaster

2018 ◽  
Vol 22 ◽  
pp. 34-39
Author(s):  
I. D. Gorodnyanski ◽  
L. I. Vorobyova
Keyword(s):  
Drosophila Melanogaster ◽  
Mobile Genetic Elements ◽  
The Body ◽  
Hybrid Dysgenesis ◽  
Specific Interactions ◽  
Host Organism ◽  
Wolbachia Pipientis ◽  
Genetic Elements

Aim. The main goal of this article was to assess changes in the frequency of the development of hybrid dysgenesis in the Oregon-R line of Drosophila melanogaster, observed under the influence of tetracycline. Methods. The line of Drosophila melanogaster Oregon R (Cambridge), in the genome of which there are full-sized and functionally active copies of the MGE hobo, was investigated. Also, this line is infected with wolbachia of the wMel strain. Removal of Wolbachia from the body was implemented using antibiotic tetracycline. The level of hybrid dysgenesis was studied by the method of extracting gonad flies and assessing their condition. Results. This article presents the results of studying the effect of suppression of the internal microflora of Drosophila melanogaster – in particular, the parasitic bacterium Wolbachia pipientis - on the level of manifestation of hybrid dysgenesis. After exposure to tetracycline, a significant increase in the proportion of dysgenic individuals within the line was observed. Conclusions. The results obtained may be associated with a violation of the system of specific interactions of the host organism and internal microflora. Keywords: mobile genetic elements, hybrid dysgenesis, Drosophila melanogaster, Wolbachia pipientis.

scholarly journals Manifestation of hybrid dysgenesis in Drosophila melanogaster Harwich line with tetracycline suppressed internal microflora

2020 ◽  
Vol 26 ◽  
pp. 46-50
Author(s):  
I. D. Gorodnyanski
Keyword(s):  
Drosophila Melanogaster ◽  
Mobile Genetic Elements ◽  
The Body ◽  
Hybrid Dysgenesis ◽  
P Element ◽  
Standard Line ◽  
Wolbachia Pipientis ◽  
Genetic Elements

Aim. To evaluate the dynamics of the manifestation of hybrid dysgenesis in the Harwich line observed under the influence of tetracycline and compare the gathered data with the data for the Oregon-R line. Methods. The line of Drosophila melanogaster Harwich was investigated. This line is considered the standard line of the P cytotype. Removal of Wolbachia from the body was implemented using antibiotic tetracycline. The level of hybrid dysgenesis was studied by the method of extracting gonad flies and assessing their condition. Results. A stable and significant increase in the proportion of dysgenic individuals within the Harwich line was recorded after suppression of the internal microflora - including Wolbachia pipientis – by tetracycline. Conclusions. The suppression of the internal microflora of Drosophila melanogaster by tetracycline significantly affects the level of manifestation of hybrid dysgenesis caused by transpositions of the P-element. The data obtained are similar to the results of a similar study of the level of manifestation of hybrid dys-genesis caused by transpositions of hobo MGE. Keywords: mobile genetic elements, hybrid dysgenesis, P-element, Drosophila melanogaster, Wolbachia pipientis.

ISSR-PCR markers and mobile genetic elements in horse (Equus caballus) genome

2014 ◽  
pp. 42-57 ◽  
Author(s):  
N.V. Bardukov ◽  
◽  
A.V. Feofilov ◽  
T.T. Glazko ◽  
V.I. Glazko ◽  
...  
Keyword(s):  
Equus Caballus ◽  
Mobile Genetic Elements ◽  
Pcr Markers ◽  
Genetic Elements ◽  
Issr Pcr
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