Construction of a Tra− deletion mutant of pAgK84 to safeguard the biological control of crown gall

1988 ◽  
Vol 212 (2) ◽  
pp. 207-214 ◽  
Author(s):  
David A. Jones ◽  
Maarten H. Ryder ◽  
Bruce G. Clare ◽  
Stephen K. Farrand ◽  
Allen Kerr
Keyword(s):  
Biological Control ◽  
Crown Gall ◽  
Deletion Mutant

Biological Control of Crown Gall Using Using Agrobacterium Strains K84 and K1026

1991 ◽  
Vol 18 (5) ◽  
pp. 571 ◽  
Author(s):  
MH Ryder ◽  
DA Jones
Keyword(s):  
Biological Control ◽  
Crown Gall ◽  
Deletion Mutant ◽  
Agrobacterium Radiobacter ◽  
Commercial Scale

Crown gall has been successfully controlled on a commercial scale for over 15 years by the use of Agrobacterium radiobacter strain K84. The continued success of strain K84 has been jeoparised by the possibility of transfer of the agrocin plasmid, pAgK84, to pathogenic agrobacteria, making them resistant to control. The construction of a transfer-deficient deletion mutant of pAgK84 has resulted in a strain (K1026) from which the agrocin plasmid (pAgK1026) can no longer be transferred to other agrobacteria. Strain K1026 controls crown gall as effectively as strain K84 and may be used to ensure that the danger of breakdown of biological control is minimised.

Biological Control of Crown Gall in Grapevine and Raspberry by Two Pseudomonas spp. with a Wide Spectrum of Antagonistic Activity

1998 ◽  
Vol 8 (1) ◽  
pp. 45-57 ◽  
Author(s):  
I. A. KHMEL ◽  
T. A. SOROKINA ◽  
N. B. LEMANOVA ◽  
V. A. LIPASOVA ◽  
O. Z. METLITSKI ◽  
...  
Keyword(s):  
Biological Control ◽  
Crown Gall ◽  
Wide Spectrum ◽  
Antagonistic Activity ◽  
Pseudomonas Spp

scholarly journals Biological Control of Crown Gall on Peach and Cherry Rootstock Colt by Native Agrobacterium radiobacter Isolates~!2009-05-11~!2009-08-25~!2010-02-11~!

2010 ◽  
Vol 3 (1) ◽  
pp. 1-10 ◽  
Author(s):  
A. K. Gupta ◽  
Kishore Khosla ◽  
S. S. Bhardwaj ◽  
Aman Thakur ◽  
Sapna Devi ◽  
...  
Keyword(s):  
Biological Control ◽  
Crown Gall ◽  
Agrobacterium Radiobacter

scholarly journals Antagonistic potential of native agrocin-producing non-pathogenic Agrobacterium tumefaciens strain UHFBA-218 to control crown gall in peach

2017 ◽  
Vol 97 (1) ◽  
pp. 1-11
Author(s):  
Aditi Sharma ◽  
A. K. Gupta ◽  
K. Khosla ◽  
Rishi Mahajan ◽  
Bharti ◽  
...  
Keyword(s):  
Biological Control ◽  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Room Temperature ◽  
Biological Control Agent ◽  
Control Agent ◽  
Stone Fruits ◽  
Copper Oxychloride ◽  
Antagonistic Potential ◽  
Different Doses

A non-pathogenic agrocin-producing native isolate ofAgrobacterium tumefaciensstrain UHFBA-218 was tested as a biological control agent against the peach crown gall. This strain was compatible with all the recommended pesticides used in stone fruits in the integrated pest management (IPM) module, except for copper oxychloride, which was detrimental to its growth. Upon artificial co-inoculation of 4-wk-old plants of tomato var. Solan Gola withA. tumefaciensstrain UHFBA-218 and tumorigenicA. tumefaciensstrain Peach 2E-10, out of the 27 isolates recovered, six were transconjugants showing selective acquisition of tumorigenic factors as made evident by amplification withiptandvirD2primers, whereas the rest of the isolates did not acquire any of these tumorigenic factors. A white stone powder-based formulation of this isolate (103.3 × 108cfu g-1) retained appreciable viability for up to 6 months at room temperature. When peach roots and seeds were soaked in cell suspensions of different doses of a white stone powder-based bioformulation of UHFBA-218 before planting in the field, the number of plants with tumours was reduced, with the lowest incidence of crown gall being observed in the 0.1% UHFBA-218 root dip treatment, i.e. 1.48% and 0.80% during the years 2013 and 2014, respectively. No incidence of crown gall was observed in the three seed dip treatments, i.e. 30-min dip in UHFBA-218 followed by 1 h of shade drying, stratified seeds dipped for 30 min in 0.1% suspensions of strains UHFBA-218 or K84 followed by 1 h of shade drying before sowing, as compared with 14.76% incidence in untreated plants.

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