Interaction between human brain tumour biopsies and fetal rat brain tissue in vitro

1990 ◽  
Vol 81 (2) ◽  
pp. 130-140 ◽  
Author(s):  
O. Engebraaten ◽  
R. Bjerkvig ◽  
M. Lund-Johansen ◽  
K. Wester ◽  
P.-H. Pedersen ◽  
...  
Keyword(s):  
Human Brain ◽  
Rat Brain ◽  
Brain Tissue ◽  
Brain Tumour ◽  
Fetal Rat ◽  
Rat Brain Tissue ◽  
Fetal Rat Brain

INTERACTIONS BETWEEN FETAL RAT BRAIN CELLS AND MATURE BRAIN TISSUE IN VIVO AND IN VITRO

1993 ◽  
Vol 52 (3) ◽  
pp. 295 ◽  
Author(s):  
Kirsten Marienhagen ◽  
Paal-Henning Pedersen ◽  
Sverre Mork ◽  
Rolf Bierkvig
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Brain Cells ◽  
Mature Brain ◽  
Fetal Rat ◽  
Fetal Rat Brain

Cryopreservation of fetal rat brain tissue later used for intracerebral transplantation

Cryobiology ◽  
1987 ◽  
Vol 24 (2) ◽  
pp. 120-134 ◽  
Author(s):  
Steen Jensen ◽  
Torben Sørensen ◽  
Jens Zimmer
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Intracerebral Transplantation ◽  
Fetal Rat ◽  
Rat Brain Tissue ◽  
Fetal Rat Brain

Interactions between fetal rat brain cells and mature brain tissue in vivo and in vitro

1994 ◽  
Vol 20 (2) ◽  
pp. 130-143 ◽  
Author(s):  
K. Marienhagen ◽  
P.-H. Pedersen ◽  
A. J. A. Terzis ◽  
O. D. Laerum ◽  
H. Arnold ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Brain Cells ◽  
Mature Brain ◽  
Fetal Rat ◽  
Fetal Rat Brain

Antenatal taurine supplementation increases taurine content in intrauterine growth restricted fetal rat brain tissue

2014 ◽  
Vol 29 (3) ◽  
pp. 867-871 ◽  
Author(s):  
Fang Li ◽  
Hui-Yun Teng ◽  
Jing Liu ◽  
Hua-Wei Wang ◽  
Li Zeng ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Taurine Supplementation ◽  
Intrauterine Growth ◽  
Fetal Rat ◽  
Rat Brain Tissue ◽  
Fetal Rat Brain

scholarly journals TGF-β1 EXPRESSION BY GLIOMA C6 CELLS IN VITRO

2017 ◽  
Vol 39 (4) ◽  
pp. 258-263 ◽  
Author(s):  
L D Liubich ◽  
L M Kovalevska ◽  
M I Lisyany ◽  
V M Semenova ◽  
T A Malysheva ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Rat Brain ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
C6 Cells ◽  
Fetal Rat ◽  
Tgf Β1 ◽  
Fetal Rat Brain

The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-β1 monoclonal antibody. Immunocytochemical staining for TGF-β1 and p53 was performed. Results: The proportion of TGF-β1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-β1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-β1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-β1 seems to be responsible for decrease in TGF-β1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS.

In vitro assessment of the agonist properties of the novel 5-HT1A receptor ligand, CUMI-101 (MMP), in rat brain tissue

2011 ◽  
Vol 38 (2) ◽  
pp. 273-277 ◽  
Author(s):  
Nicola Hendry ◽  
Isabel Christie ◽  
Eugenii Alfredovich Rabiner ◽  
Marc Laruelle ◽  
Jeannette Watson
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
The Novel ◽  
Receptor Ligand ◽  
In Vitro Assessment ◽  
Rat Brain Tissue

scholarly journals Nerve growth cones isolated from fetal rat brain. IV. Preparation of a membrane subfraction and identification of a membrane glycoprotein expressed on sprouting neurons.

1985 ◽  
Vol 101 (5) ◽  
pp. 1977-1989 ◽  
Author(s):  
L Ellis ◽  
I Wallis ◽  
E Abreu ◽  
K H Pfenninger
Keyword(s):  
Rat Brain ◽  
Fetal Brain ◽  
Neurite Growth ◽  
Membrane Glycoprotein ◽  
Polyacrylamide Gels ◽  
Nerve Growth ◽  
Adult Rat ◽  
Fetal Rat ◽  
Fetal Rat Brain

This study describes the preparation of a membrane subfraction from isolated nerve growth cone particles (GCPs) (see Pfenninger, K. H., L. Ellis, M. P. Johnson, L. B. Friedman, and S. Somlo, 1983, Cell, 35:573-584) and the identification in this fraction of a glycoprotein expressed during neurite growth. While approximately 40 major polypeptides are visible in Coomassie Blue-stained SDS polyacrylamide gels of pelleted (partially disrupted) GCPs, a salt-washed membrane fraction prepared from lysed, detergent-permeabilized GCPs contains only 14% of this protein and has an unusually simple polypeptide pattern of seven major bands. Monoclonal antibodies have been generated to GCP membranes isolated from fetal rat brain. These antibodies have been screened differentially with synaptosomes from adult rat brain in order to identify those which recognize antigens expressed selectively during neurite growth. One such antibody (termed 5B4) recognizes a developmentally regulated membrane glycoprotein that is enriched in GCP membranes and expressed in fetal neurons sprouting in vitro. The 5B4 antigen in fetal brain migrates in SDS polyacrylamide gels as a diffuse band of approximately 185-255 kD, is rich in sialic acid, and consists of a small family of isoelectric variants. Freezing-thawing and neuraminidase digestion result in the cleavage of the native antigen into two new species migrating diffusely around 200 and 160 kD. Prolonged neuraminidase digestion sharpens these bands at about 180 and 135 kD, respectively. In the mature brain, antibody 5B4 recognizes a sparse polypeptide migrating at approximately 140 kD. As shown in the following paper (Wallis, I., L. Ellis, K. Suh, and K. H. Pfenninger, 1985, J. Cell Biol., 101:1990-1998), the fetal antigen is specifically associated with regions of neuronal sprouting and, therefore, can be used as a molecular marker of neurite growth.

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