Molecular weight distribution of the aminoacyl-tRNA-synthetases of Escherichia coli by gel filtration

1967 ◽  
Vol 100 (4) ◽  
pp. 378-382 ◽  
Author(s):  
Gisela Nass ◽  
Georg Stöffler
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Gel Filtration ◽  
Trna Synthetases ◽  
Aminoacyl Trna Synthetases

scholarly journals Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

Marine Drugs ◽  
2019 ◽  
Vol 17 (12) ◽  
pp. 694 ◽  
Author(s):  
Yixiang Liu ◽  
Wenqiang Liu ◽  
Yanbo Wang ◽  
Yu Ma ◽  
Ling Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Monosaccharide Composition ◽  
Inhibitory Effect ◽  
Enterotoxigenic Escherichia Coli ◽  
Sulfated Polysaccharides ◽  
Natural Polymers ◽  
Sulfated Galactans

Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics. This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88. Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88. A structure–activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition. In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides. Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion. In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.

scholarly journals Molecular Weight Distribution Studies on Heparin

1977 ◽  
Author(s):  
Grant H. Barlow
Keyword(s):  
Molecular Weight ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Gel Filtration ◽  
Equilibrium Analysis ◽  
Resolving Power ◽  
Sedimentation Equilibrium ◽  
Distribution Data ◽  
Molecular Weights ◽  
Distribution Studies

The determination of molecular weight distribution using the sedimentation equilibrium analysis developed for polymers by T. Scholte (J. Polymer Sei, 6, 111, 1968) has been adapted for heparin analysis. Pork mucosal heparin separated into molecular weight subfractions by gel filtration on Ultrogel AcA44 (L.K.B.) was used to test the validity and resolving power of the method. Results indicate that the method is able to differentiate molecular weight distribution satisfactorily. Comparisons have been made of molecular weight distribution of samples from different species, organs and manufacturers. Average molecular weights for most samples center around 15,000 Dal tons, but samples show considerable variation in their distribution data. Results suggest that variations between manufacturers is more pronounced than the specie and organ difference indicating the importance of the purification procedure.

Determination of molecular-weight distribution of aorta glycosaminoglycans by automated gel filtration

1980 ◽  
Vol 192 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Bhandaru Radhakrishnamurthy ◽  
Edward Rdalferes ◽  
Parakat Vijayagopal ◽  
Gerald S. Berenson
Keyword(s):  
Molecular Weight ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Gel Filtration
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