Punctuation of transcription in vitro of the tryptophan operon of Escherichia coli

1975 ◽  
Vol 136 (3) ◽  
pp. 199-214 ◽  
Author(s):  
Hans Pannekoek ◽  
William J. Brammar ◽  
Peter H. Pouwels
Keyword(s):  
Escherichia Coli ◽  
Tryptophan Operon ◽  
Transcription In Vitro

Termination of transcription in vitro in the escherichia coli tryptophan operon leader region

1976 ◽  
Vol 103 (2) ◽  
pp. 383-393 ◽  
Author(s):  
Frank Lee ◽  
Catherine L. Squires ◽  
Craig Squires ◽  
Charles Yanofsky
Keyword(s):  
Escherichia Coli ◽  
Leader Region ◽  
Tryptophan Operon ◽  
Transcription In Vitro

The specificity of transcription in vitro of the tryptophan operon of Escherichia coli

1974 ◽  
Vol 132 (4) ◽  
pp. 291-306 ◽  
Author(s):  
Hans Pannekoek ◽  
Bernard Perbal ◽  
Peter Pouwels
Keyword(s):  
Escherichia Coli ◽  
Tryptophan Operon ◽  
Transcription In Vitro

scholarly journals DksA Is Required for Growth Phase-Dependent Regulation, Growth Rate-Dependent Control, and Stringent Control of fis Expression in Escherichia coli

2006 ◽  
Vol 188 (16) ◽  
pp. 5775-5782 ◽  
Author(s):  
Prabhat Mallik ◽  
Brian J. Paul ◽  
Steven T. Rutherford ◽  
Richard L. Gourse ◽  
Robert Osuna
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Amino Acid ◽  
Rna Polymerase ◽  
Growth Phase ◽  
Stationary Growth ◽  
Rate Dependent ◽  
Transcription In Vitro ◽  
State Growth

ABSTRACT DksA is a critical transcription factor in Escherichia coli that binds to RNA polymerase and potentiates control of rRNA promoters and certain amino acid promoters. Given the kinetic similarities between rRNA promoters and the fis promoter (Pfis), we investigated the possibility that DksA might also control transcription from Pfis. We show that the absence of dksA extends transcription from Pfis well into the late logarithmic and stationary growth phases, demonstrating the importance of DksA for growth phase-dependent regulation of fis. We also show that transcription from Pfis increases with steady-state growth rate and that dksA is absolutely required for this regulation. In addition, both DksA and ppGpp are required for inhibition of Pfis promoter activity following amino acid starvation, and these factors act directly and synergistically to negatively control Pfis transcription in vitro. DksA decreases the half-life of the intrinsically short-lived fis promoter-RNA polymerase complex and increases its sensitivity to the concentration of CTP, the predominant initiating nucleotide triphosphate for this promoter. This work extends our understanding of the multiple factors controlling fis expression and demonstrates the generality of the DksA requirement for regulation of kinetically similar promoters.

scholarly journals Mutational Analysis of the Chlamydia trachomatis rRNA P1 Promoter Defines Four Regions Important for Transcription In Vitro

1998 ◽  
Vol 180 (9) ◽  
pp. 2359-2366 ◽  
Author(s):  
Ming Tan ◽  
Tamas Gaal ◽  
Richard L. Gourse ◽  
Joanne N. Engel
Keyword(s):  
Escherichia Coli ◽  
Chlamydia Trachomatis ◽  
Rna Polymerase ◽  
Mutational Analysis ◽  
In Vitro Transcription ◽  
Rna Polymerases ◽  
Rich Region ◽  
E Coli ◽  
Transcription In Vitro

ABSTRACT We have characterized the Chlamydia trachomatisribosomal promoter, rRNA P1, by measuring the effect of substitutions and deletions on in vitro transcription with partially purifiedC. trachomatis RNA polymerase. Our analyses indicate that rRNA P1 contains potential −10 and −35 elements, analogous toEscherichia coli promoters recognized by E-ς70. We identified a novel AT-rich region immediately downstream of the −35 region. The effect of this region was specific for C. trachomatis RNA polymerase and strongly attenuated by single G or C substitutions. Upstream of the −35 region was an AT-rich sequence that enhanced transcription by C. trachomatis and E. coli RNA polymerases. We propose that this region functions as an UP element.

Involvement of the nusA and nusB gene products in transcription of Escherichia coli tryptophan operon in vitro

1982 ◽  
Vol 185 (2) ◽  
pp. 369-371 ◽  
Author(s):  
Kazuyuki Kuroki ◽  
Shunsuke Ishii ◽  
Yasunobu Kano ◽  
Tomoyuki Miyashita ◽  
Kayoko Nishi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Products ◽  
Tryptophan Operon

scholarly journals Autonomous Role of 3′-Terminal CCCA in Directing Transcription of RNAs by Qβ Replicase

2001 ◽  
Vol 75 (23) ◽  
pp. 11373-11383 ◽  
Author(s):  
David M. Tretheway ◽  
Shigeo Yoshinari ◽  
Theo W. Dreher
Keyword(s):  
Escherichia Coli ◽  
Secondary Structure ◽  
Similar Sequence ◽  
Transcriptional Initiation ◽  
Template Sequence ◽  
Transcription In Vitro ◽  
Internal Initiation

ABSTRACT We have studied transcription in vitro by Qβ replicase to deduce the minimal features needed for efficient end-to-end copying of an RNA template. Our studies have used templates ca. 30 nucleotides long that are expected to be free of secondary structure, permitting unambiguous analysis of the role of template sequence in directing transcription. A 3′-terminal CCCA (3′-CCCA) directs transcriptional initiation to opposite the underlined C; the amount of transcription is comparable between RNAs possessing upstream (CCA) n tracts, A-rich sequences, or a highly folded domain and is also comparable in single-round transcription assays to transcription of two amplifiable RNAs. Predominant initiation occurs within the 3′-CCCA initiation box when a wide variety of sequences is present immediately upstream, but CCA or a closely similar sequence in that position results in significant internal initiation. Removal of the 3′-A from the 3′-CCCA results in 5- to 10-fold-lower transcription, emphasizing the importance of the nontemplated addition of 3′-A by Qβ replicase during termination. In considering whether 3′-CCCA could provide sufficient specificity for viral transcription, and consequently amplification, in vivo, we note that tRNAHis is the only stable Escherichia coliRNA with 3′-CCCA. In vitro-generated transcripts corresponding to tRNAHis served as poor templates for Qβ replicase; this was shown to be due to the inaccessibility of the partially base-paired CCCA. These studies demonstrate that 3′-CCCA plays a major role in the control of transcription by Qβ replicase and that the abundant RNAs present in the host cell should not be efficient templates.

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