Involvement of cyclic AMP and its receptor protein in the sensitivity of Escherichia coli K 12 toward serine

1979 ◽  
Vol 176 (3) ◽  
pp. 343-350 ◽  
Author(s):  
Jacques Daniel ◽  
Antoine Danchin
Keyword(s):  
Escherichia Coli ◽  
Cyclic Amp ◽  
Receptor Protein ◽  
K 12

scholarly journals The cyclic AMP (cAMP)-cAMP receptor protein complex functions both as an activator and as a corepressor at the tsx-p2 promoter of Escherichia coli K-12.

1991 ◽  
Vol 173 (17) ◽  
pp. 5419-5430 ◽  
Author(s):  
P Gerlach ◽  
L Søgaard-Andersen ◽  
H Pedersen ◽  
J Martinussen ◽  
P Valentin-Hansen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cyclic Amp ◽  
Protein Complex ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Complex Functions ◽  
P2 Promoter ◽  
Camp Receptor ◽  
K 12

scholarly journals YeeI, a Novel Protein Involved in Modulation of the Activity of the Glucose-Phosphotransferase System in Escherichia coli K-12

2006 ◽  
Vol 188 (15) ◽  
pp. 5439-5449 ◽  
Author(s):  
Ann-Katrin Becker ◽  
Tim Zeppenfeld ◽  
Ariane Staab ◽  
Sabine Seitz ◽  
Winfried Boos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Transduction ◽  
Cyclic Amp ◽  
Glucose Transporter ◽  
Signal Transduction Pathway ◽  
Receptor Protein ◽  
Phosphotransferase System ◽  
Reading Frame ◽  
K 12 ◽  
Novel Protein

ABSTRACT The membrane-bound protein EIICBGlc encoded by the ptsG gene is the major glucose transporter in Escherichia coli. This protein is part of the phosphoenolpyruvate:glucose-phosphotransferase system, a very important transport and signal transduction system in bacteria. The regulation of ptsG expression is very complex. Among others, two major regulators, the repressor Mlc and the cyclic AMP-cyclic AMP receptor protein activator complex, have been identified. Here we report identification of a novel protein, YeeI, that is involved in the regulation of ptsG by interacting with Mlc. Mutants with reduced activity of the glucose-phosphotransferase system were isolated by transposon mutagenesis. One class of mutations was located in the open reading frame yeeI at 44.1 min on the E. coli K-12 chromosome. The yeeI mutants exhibited increased generation times during growth on glucose, reduced transport of methyl-α-d-glucopyranoside, a substrate of EIICBGlc, reduced induction of a ptsG-lacZ operon fusion, and reduced catabolite repression in lactose/glucose diauxic growth experiments. These observations were the result of decreased ptsG expression and a decrease in the amount of EIICBGlc. In contrast, overexpression of yeeI resulted in higher expression of ptsG, of a ptsG-lacZ operon fusion, and of the autoregulated dgsA gene. The effect of a yeeI mutation could be suppressed by introducing a dgsA deletion, implying that the two proteins belong to the same signal transduction pathway and that Mlc is epistatic to YeeI. By measuring the surface plasmon resonance, we found that YeeI (proposed gene designation, mtfA) directly interacts with Mlc with high affinity.

scholarly journals Correlation between Growth Rates, EIIACrr Phosphorylation, and Intracellular Cyclic AMP Levels in Escherichia coli K-12

2007 ◽  
Vol 189 (19) ◽  
pp. 6891-6900 ◽  
Author(s):  
Katja Bettenbrock ◽  
Thomas Sauter ◽  
Knut Jahreis ◽  
Andreas Kremling ◽  
Joseph W. Lengeler ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cyclic Amp ◽  
Growth Rates ◽  
Catabolite Repression ◽  
Cellular Growth ◽  
Receptor Protein ◽  
Intracellular Camp ◽  
Global Control ◽  
K 12

ABSTRACT In Escherichia coli K-12, components of the phosphoenolpyruvate-dependent phosphotransferase systems (PTSs) represent a signal transduction system involved in the global control of carbon catabolism through inducer exclusion mediated by phosphoenolpyruvate-dependent protein kinase enzyme IIACrr (EIIACrr) (= EIIAGlc) and catabolite repression mediated by the global regulator cyclic AMP (cAMP)-cAMP receptor protein (CRP). We measured in a systematic way the relation between cellular growth rates and the key parameters of catabolite repression, i.e., the phosphorylated EIIACrr (EIIACrr∼P) level and the cAMP level, using in vitro and in vivo assays. Different growth rates were obtained by using either various carbon sources or by growing the cells with limited concentrations of glucose, sucrose, and mannitol in continuous bioreactor experiments. The ratio of EIIACrr to EIIACrr∼P and the intracellular cAMP concentrations, deduced from the activity of a cAMP-CRP-dependent promoter, correlated well with specific growth rates between 0.3 h−1 and 0.7 h−1, corresponding to generation times of about 138 and 60 min, respectively. Below and above this range, these parameters were increasingly uncoupled from the growth rate, which perhaps indicates an increasing role executed by other global control systems, in particular the stringent-relaxed response system.

scholarly journals The CytR repressor antagonizes cyclic AMP-cyclic AMP receptor protein activation of the deoCp2 promoter of Escherichia coli K-12.

1990 ◽  
Vol 172 (10) ◽  
pp. 5706-5713 ◽  
Author(s):  
L Søgaard-Andersen ◽  
J Martinussen ◽  
N E Møllegaard ◽  
S R Douthwaite ◽  
P Valentin-Hansen
Keyword(s):  
Escherichia Coli ◽  
Cyclic Amp ◽  
Receptor Protein ◽  
Cyclic Amp Receptor Protein ◽  
Protein Activation ◽  
K 12
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