Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae

1984 ◽  
Vol 195 (1-2) ◽  
pp. 29-34 ◽  
Author(s):  
Yasuhisa Nogi ◽  
Hideo Shimada ◽  
Yuriko Matsuzaki ◽  
Hideaki Hashimoto ◽  
Toshio Fukasawa
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Cluster ◽  
Regulation Of Expression

Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae

1983 ◽  
Vol 191 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Hideaki Hashimoto ◽  
Yoshiko Kikuchi ◽  
Yasuhisa Nogi ◽  
Toshio Fukasawa
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Cluster ◽  
Regulation Of Expression

scholarly journals Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (8) ◽  
pp. 1440-1448 ◽  
Author(s):  
M Johnston ◽  
R W Davis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Sequence ◽  
Base Pair ◽  
Transcription Initiation ◽  
Base Pairs ◽  
Regulation Of Expression ◽  
Notable Feature ◽  
His3 Gene

The GAL1 and GAL10 genes of Saccharomyces cerevisiae are divergently transcribed, with 606 base pairs of DNA separating their transcription initiation sites. These two genes are stringently coregulated: their expression is induced ca. 1,000-fold in cells growing on galactose and is repressed by growth on glucose. The nucleotide sequence of the region of DNA between these genes and the precise sites of transcription initiation are presented here. The most notable feature of the nucleotide sequence of this region is a 108-base-pair guanine-plus-cytosine-rich stretch of DNA located approximately in the middle of the region between GAL1 and GAL10. Analysis of the effects of mutations that alter the region between these two genes, constructed in vitro or selected in vivo, suggest that these guanine-plus-cytosine-rich sequences are required for the expression of both genes. The region of DNA between GAL1 and GAL10 is sufficient for regulation of expression of these genes: fusion of the region to the yeast HIS3 gene places HIS3 under GAL control.

scholarly journals Molecular cloning of the yeast mitochondrial aconitase gene (ACO1) and evidence of a synergistic regulation of expression by glucose plus glutamate.

1990 ◽  
Vol 10 (7) ◽  
pp. 3551-3561 ◽  
Author(s):  
S P Gangloff ◽  
D Marguet ◽  
G J Lauquin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Nucleotide Sequence ◽  
Glucose Repression ◽  
Regulation Of Expression ◽  
Enzymatic Assays ◽  
Molecular Analyses ◽  
Mitochondrial Aconitase ◽  
Synergistic Regulation ◽  
Mammalian Protein

We have isolated genomic clones complementing the aconitase-deficient strain (glu1-1) of Saccharomyces cerevisiae. Identification of the aconitase gene was established by enzymatic assays and molecular analyses. The corresponding mRNA has been characterized, and its direction of transcription has been determined. The complete nucleotide sequence revealed strong amino acid homologies with the sequences of some peptides isolated from the mammalian protein. Disruption of the gene by deletion-insertion led to glutamate auxotrophy. Expression of the aconitase gene was sensitive to glucose repression and was synergistically down regulated by glucose and glutamate.

scholarly journals The nif Gene Operon of the Methanogenic Archaeon Methanococcus maripaludis

1998 ◽  
Vol 180 (6) ◽  
pp. 1504-1511 ◽  
Author(s):  
Peter S. Kessler ◽  
Carrine Blank ◽  
John A. Leigh
Keyword(s):  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Sequence Similarity ◽  
Methanogenic Archaea ◽  
Gene Clusters ◽  
Northern Analysis ◽  
Insertion Mutagenesis ◽  
Fragmentation Pattern ◽  
Methanococcus Maripaludis ◽  
Regulation Of Expression

ABSTRACT Nitrogen fixation occurs in two domains, Archaea andBacteria. We have characterized a nif(nitrogen fixation) gene cluster in the methanogenic archaeonMethanococcus maripaludis. Sequence analysis revealed eight genes, six with sequence similarity to known nif genes and two with sequence similarity to glnB. The gene order,nifH, ORF105 (similar to glnB),ORF121 (similar to glnB), nifD,nifK, nifE, nifN, andnifX, was the same as that found in part in other diazotrophic methanogens and except for the presence of theglnB-like genes, also resembled the order found in many members of the Bacteria. Using transposon insertion mutagenesis, we determined that an 8-kb region required for nitrogen fixation corresponded to the nif gene cluster. Northern analysis revealed the presence of either a single 7.6-kbnif mRNA transcript or 10 smaller mRNA species containing portions of the large transcript. Polar effects of transposon insertions demonstrated that all of these mRNAs arose from a single promoter region, where transcription initiated 80 bp 5′ tonifH. Distinctive features of the nif gene cluster include the presence of the six primary nif genes in a single operon, the placement of the two glnB-like genes within the cluster, the apparent physical separation of the cluster from any other nif genes that might be in the genome, the fragmentation pattern of the mRNA, and the regulation of expression by a repression mechanism described previously. Our study and others with methanogenic archaea reporting multiple mRNAs arising from gene clusters with only a single putative promoter sequence suggest that mRNA processing following transcription may be a common occurrence in methanogens.

Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae

1984 ◽  
Vol 4 (8) ◽  
pp. 1440-1448
Author(s):  
M Johnston ◽  
R W Davis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Sequence ◽  
Base Pair ◽  
Transcription Initiation ◽  
Base Pairs ◽  
Regulation Of Expression ◽  
Notable Feature ◽  
His3 Gene

The GAL1 and GAL10 genes of Saccharomyces cerevisiae are divergently transcribed, with 606 base pairs of DNA separating their transcription initiation sites. These two genes are stringently coregulated: their expression is induced ca. 1,000-fold in cells growing on galactose and is repressed by growth on glucose. The nucleotide sequence of the region of DNA between these genes and the precise sites of transcription initiation are presented here. The most notable feature of the nucleotide sequence of this region is a 108-base-pair guanine-plus-cytosine-rich stretch of DNA located approximately in the middle of the region between GAL1 and GAL10. Analysis of the effects of mutations that alter the region between these two genes, constructed in vitro or selected in vivo, suggest that these guanine-plus-cytosine-rich sequences are required for the expression of both genes. The region of DNA between GAL1 and GAL10 is sufficient for regulation of expression of these genes: fusion of the region to the yeast HIS3 gene places HIS3 under GAL control.

scholarly journals A novel rat homolog of the Saccharomyces cerevisiae ubiquitin-conjugating enzymes UBC4 and UBC5 with distinct biochemical features is induced during spermatogenesis.

1996 ◽  
Vol 16 (8) ◽  
pp. 4064-4072 ◽  
Author(s):  
S S Wing ◽  
N Bédard ◽  
C Morales ◽  
P Hingamp ◽  
J Trasler
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Similarity ◽  
Amino Acid Identity ◽  
Cellular Function ◽  
Rat Tissues ◽  
Regulation Of Expression ◽  
Conjugating Enzymes ◽  
Ubiquitin Conjugating Enzymes ◽  
First Time

The Saccharomyces cerevisiae ubiquitin-conjugating enzymes (E2s) UBC4 and UBC5 are essential for degradation of short-lived and abnormal proteins. We previously identified rat cDNAs encoding two E2s with strong sequence similarity to UBC4 and UBC5. These E2 isoforms are widely expressed in rat tissues, consistent with a fundamental cellular function for these E2s. We now report a new isoform, 8A, which despite having >91% amino acid identity with the other isoforms, shows several novel features. Expression of the 8A isoform appears restricted to the testis, is absent in early life, but is induced during puberty. Hypophysectomy reduced expression of the 8A isoform. In situ hybridization studies indicated that 8A mRNA is expressed mainly in round spermatids. Immunoblot analyses showed that 8A protein is found not only in subfractions of germ cells enriched in round spermatids but also in subfractions containing residual bodies extruded from more mature elongated spermatids, indicating that the protein possesses a longer half-life than the mRNA. Unlike all previously identified mammalian and plant homologs of S. cerevisiae UBC4, which possess a basic pI, the 8A isoform is unique in possessing an acidic pI. The small differences in sequence between the 8A isoform and other rat isoforms conferred differences in biochemical function. The 8A isoform was less effective than an isoform with a basic pI or ineffective in conjugating ubiquitin to certain fractions of testis proteins. Thus, although multiple isoforms of a specific E2 may exist to ensure performance of a critical cellular function, our data demonstrate, for the first time, that multiple genes also permit highly specialized regulation of expression of specific isoforms and that subtle differences in E2 primary structure can dictate conjugation of ubiquitin to different subsets of cellular proteins.

scholarly journals Replicative Age Induces Mitotic Recombination in the Ribosomal RNA Gene Cluster of Saccharomyces cerevisiae

PLoS Genetics ◽  
2011 ◽  
Vol 7 (3) ◽  
pp. e1002015 ◽  
Author(s):  
Derek L. Lindstrom ◽  
Christina K. Leverich ◽  
Kiersten A. Henderson ◽  
Daniel E. Gottschling
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Cluster ◽  
Ribosomal Rna ◽  
Mitotic Recombination ◽  
Ribosomal Rna Gene
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