Cloning of rpsO, the gene for ribosomal protein S15 of Escherichia coli

1982 ◽  
Vol 188 (2) ◽  
pp. 334-337 ◽  
Author(s):  
Renkichi Takata ◽  
Michiko Aoyagi ◽  
Tsunehiro Mukai
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Protein ◽  
Ribosomal Protein S15

Nucleotide sequence of the gene for Escherichia coli ribosomal protein S15 (rpsO)

1984 ◽  
Vol 197 (2) ◽  
pp. 225-229 ◽  
Author(s):  
Renkichi Takata ◽  
Tsunehiro Mukai ◽  
Michiko Aoyagi ◽  
Katsuji Hori
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Ribosomal Protein ◽  
Ribosomal Protein S15

Translational autocontrol of the Escherichia coli ribosomal protein S15

1990 ◽  
Vol 211 (2) ◽  
pp. 407-414 ◽  
Author(s):  
Claude Portier ◽  
Liliane Dondon ◽  
Marianne Grunberg-Manago
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Protein ◽  
Ribosomal Protein S15

A pseudoknot is required for efficient translational initiation and regulation of the Escherichia coli rpsO gene coding for ribosomal protein S15

1995 ◽  
Vol 73 (11-12) ◽  
pp. 1131-1140 ◽  
Author(s):  
Chantal Ehresmann ◽  
Claude Philippe ◽  
E. Westhof ◽  
Bernard Ehresmann ◽  
Lionel Bénard ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Ribosomal Protein ◽  
Translational Control ◽  
Mutational Analysis ◽  
Unexpected Result ◽  
Structural Element ◽  
Translational Initiation ◽  
Translational Operator ◽  
Ribosomal Protein S15

Escherichia coli ribosomal protein S15 down regulates its own synthesis by binding to its mRNA in a region overlapping the ribosome binding site, called the translational operator. This binding stabilizes a pseudoknot structure that exists in equilibrium with two stem–loop structures. When synthesized in excess over 16S rRNA, S15 binds to its translational operator and traps the ribosome on its loading site in a transient state, preventing the formation of the active ternary (30S–mRNA–rRNAfMet) complex. This inhibition can be suppressed by 16S rRNA, which displaces S15 from the mRNA. An extensive mutational analysis showed that the pseudoknot is the structural element required for S15 recognition and in vivo translational control. Specific sequence determinants are located in limited regions of the structure formed by the pseudoknot. An unexpected result is that the pseudoknot can exist in a variety of topologically equivalent structures recognizable and shapable by S15. Based on footprinting experiments and computer graphic modelling, S15 shields the two stems of the pseudoknot, sitting in the major groove of the coaxial stack.Key words: ribosomes, translational control, r-protein S15, pseudoknot, RNA–protein recognition.

scholarly journals Ribosomal protein S15 from Escherichia coli modulates its own translation by trapping the ribosome on the mRNA initiation loading site.

1993 ◽  
Vol 90 (10) ◽  
pp. 4394-4398 ◽  
Author(s):  
C. Philippe ◽  
F. Eyermann ◽  
L. Benard ◽  
C. Portier ◽  
B. Ehresmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Protein ◽  
Ribosomal Protein S15

Target site of Escherichia coli ribosomal protein S15 on its messenger RNA

1990 ◽  
Vol 211 (2) ◽  
pp. 415-426 ◽  
Author(s):  
Claude Philippe ◽  
Claude Portier ◽  
Maryléne Mougel ◽  
Marianne Grunberg-Manago ◽  
Jean Pierre Ebel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Protein ◽  
Messenger Rna ◽  
Target Site ◽  
Ribosomal Protein S15
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