scholarly journals Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli

1989 ◽  
Vol 218 (3) ◽  
pp. 516-522 ◽  
Author(s):  
Herbert P. Schweizer ◽  
Prasanta Datta
Keyword(s):  
Escherichia Coli ◽  
Dna Sequence ◽  
Regulatory Gene ◽  
Tdc Operon

scholarly journals DNA sequence of the araC regulatory gene from Escherichia coli B/r

1980 ◽  
Vol 8 (22) ◽  
pp. 5267-5274 ◽  
Author(s):  
C.Garrett Miyada ◽  
Arnold H. Horwitz ◽  
Laura G. Cass ◽  
Josef Timko ◽  
Gary Wilcox
Keyword(s):  
Escherichia Coli ◽  
Dna Sequence ◽  
Regulatory Gene ◽  
Escherichia Coli B

scholarly journals Further tests of a recombination model in which chi removes the RecD subunit from the RecBCD enzyme of Escherichia coli.

Genetics ◽  
1990 ◽  
Vol 126 (3) ◽  
pp. 519-533 ◽  
Author(s):  
F W Stahl ◽  
L C Thomason ◽  
I Siddiqi ◽  
M M Stahl
Keyword(s):  
Escherichia Coli ◽  
Dna Sequence ◽  
Dna Packaging ◽  
Lambda Phage ◽  
Recombination Model ◽  
Phage Types ◽  
Point Of Entry ◽  
Reciprocal Recombination ◽  
Recbcd Enzyme ◽  
Lambda Dna

Abstract When one of two infecting lambda phage types in a replication-blocked cross is chi + and DNA packaging is divorced from the RecBCD-chi interaction, complementary chi-stimulated recombinants are recovered equally in mass lysates only if the chi + parent is in excess in the infecting parental mixture. Otherwise, the chi 0 recombinant is recovered in excess. This observation implies that, along with the chi 0 chromosome, two chi + parent chromosomes are involved in the formation of each chi + recombinant. The trimolecular nature of chi +-stimulated recombination is manifest in recombination between lambda and a plasmid. When lambda recombines with a plasmid via the RecBCD pathway, the resulting chromosome has an enhanced probability of undergoing lambda x lambda recombination in the interval into which the plasmid was incorporated. These two observations support a model in which DNA is degraded by Exo V from cos, the sequence that determines the end of packaged lambda DNA and acts as point of entry for RecBCD enzyme, to chi, the DNA sequence that stimulates the RecBCD enzyme to effect recombination. The model supposes that chi acts by ejecting the RecD subunit from the RecBCD enzyme with two consequences. (1) ExoV activity is blocked leaving a highly recombinagenic, frayed duplex end near chi, and (2) as the enzyme stripped of the RecD subunit travels beyond chi it is competent to catalyze reciprocal recombination.

DNA sequence and analysis of a 90.1-kb plasmid in Shiga toxin-producing Escherichia coli (STEC) O145:NM 83-75

Plasmid ◽  
2012 ◽  
Vol 68 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Xianghe Yan ◽  
Pina M. Fratamico ◽  
David S. Needleman ◽  
Darrell O. Bayles
Keyword(s):  
Escherichia Coli ◽  
Dna Sequence ◽  
Shiga Toxin

Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases

1988 ◽  
Vol 8 (8) ◽  
pp. 3439-3447 ◽  
Author(s):  
W Bajwa ◽  
T E Torchia ◽  
J E Hopper
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Regulatory Gene ◽  
Data Bank ◽  
Noncoding Region ◽  
Striking Similarity ◽  
Coding Region ◽  
Reading Frame ◽  
Carbon Regulation ◽  
Sequence Similarities

GAL3 gene expression is required for rapid GAL4-mediated galactose induction of the galactose-melibiose regulon genes in Saccharomyces cerevisiae. Here we show by Northern (RNA) blot analysis that GAL3 gene expression is itself galactose inducible. Like the GAL1, GAL7, GAL10, and MEL1 genes, the GAL3 gene is severely glucose repressed. Like the MEL1 gene, but in contrast to the GAL1, GAL7, and GAL10 genes, GAL3 is expressed at readily detectable basal levels in cells grown in noninducing, nonrepressing media. We determined the sequence of the S. cerevisiae GAL3 gene and its 5'-noncoding region. Within the 5'-noncoding region of the GAL3 gene, we found two sequences similar to the UASGal elements of the other galactose-melibiose regulon genes. Deletion analysis indicated that only the most ATG proximal of these sequences is required for GAL3 expression. The coding region of GAL3 consists of a 1,275-base-pair open reading frame in the direction of transcription. A comparison of the deduced 425-amino-acid sequence with the protein data bank revealed three regions of striking similarity between the GAL3 protein and the GAL1-specified galactokinase of Saccharomyces carlsbergensis. One of these regions also showed striking similarity to sequences within the galactokinase protein of Escherichia coli. On the basis of these protein sequence similarities, we propose that the GAL3 protein binds a molecule identical to or structurally related to one of the substrates or products of the galactokinase-catalyzed reaction.

The DNA sequence on bacteriophage G4 recognized by the Escherichia coli B restriction enzyme

1979 ◽  
Vol 131 (4) ◽  
pp. 871-875 ◽  
Author(s):  
James A. Lautenberger ◽  
Marshall H. Edgell ◽  
Clyde A. Hutchison ◽  
G.Nigel Godson
Keyword(s):  
Escherichia Coli ◽  
Dna Sequence ◽  
Restriction Enzyme ◽  
Escherichia Coli B

Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone

Nature ◽  
1979 ◽  
Vol 281 (5732) ◽  
pp. 544-548 ◽  
Author(s):  
David V. Goeddel ◽  
Herbert L. Heyneker ◽  
Toyohara Hozumi ◽  
Rene Arentzen ◽  
Keiichi Itakura ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Hormone ◽  
Dna Sequence ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Expression In Escherichia Coli
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