052 Purification of 6-phosphofructokinase by triazine dye affinity chromatography

1992 ◽  
Vol 343 (1) ◽  
pp. 106-107 ◽  
Author(s):  
M. Legiša
1982 ◽  
Vol 203 (3) ◽  
pp. 699-705 ◽  
Author(s):  
M D Scawen ◽  
J Darbyshire ◽  
M J Harvey ◽  
T Atkinson

3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two sequential affinity-chromatography steps on two triazine dye-Sepharose matrices. Recoveries of both enzymes were in excess of 60%. Malate dehydrogenase could also be purified by a combination of triazine dye affinity chromatography and gel filtration on Ultrogel AcA-44, but this offered no significant advantage over the purely affinity procedure.


1981 ◽  
Vol 9 (4) ◽  
pp. 290-293 ◽  
Author(s):  
TONY ATKINSON ◽  
PETER M. HAMMOND ◽  
ROY D. HARTWELL ◽  
PETER HUGHES ◽  
MICHAEL D. SCAWEN ◽  
...  

1995 ◽  
Vol 710 (2) ◽  
pp. 259-266 ◽  
Author(s):  
Igor Yu. Galaev ◽  
Pär Arvidsson ◽  
Bo Mattiasson

1996 ◽  
Vol 10 (3) ◽  
pp. 211-214 ◽  
Author(s):  
Silvia A. Camperi ◽  
Mariano Grasselli ◽  
Osvaldo Cascone

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