Balbiani ring RNA in the cytoplasm of Chironomus thummi salivary gland cells

Chromosoma ◽  
1978 ◽  
Vol 66 (3) ◽  
pp. 205-223 ◽  
Author(s):  
Edgar Serfling ◽  
Antje Huth
Keyword(s):  
Salivary Gland ◽  
Balbiani Ring ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Chironomus Thummi

Microinjection of anti-topoisomerase I immunoglobulin G into nuclei of Chironomus tentans salivary gland cells leads to blockage of transcription elongation

1987 ◽  
Vol 7 (12) ◽  
pp. 4308-4316
Author(s):  
E Egyházi ◽  
E Durban
Keyword(s):  
Salivary Gland ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Immunoglobulin G ◽  
Topoisomerase I ◽  
Chironomus Tentans ◽  
Balbiani Ring ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Transcriptional Process

Purified anti-topoisomerase I immunoglobulin G (IgG) was microinjected into nuclei of Chironomus tentans salivary gland cells, and the effect on DNA transcription was investigated. Synthesis of nucleolar preribosomal 38S RNA by RNA polymerase I and of chromosomal Balbiani ring RNA by RNA polymerase II was inhibited by about 80%. The inhibitory action of anti-topoisomerase I IgG could be reversed by the addition of exogenous topoisomerase I. Anti-topoisomerase I IgG had less effect on RNA polymerase II-promoted activity of other less efficiently transcribing heterogeneous nuclear RNA genes. The pattern of inhibition of growing nascent Balbiani ring chains indicated that the transcriptional process was interrupted at the level of chain elongation. The highly decondensed state of active Balbiani ring chromatin, however, remained unaffected after injection of topoisomerase I antibodies. These data are consistent with the interpretation that topoisomerase I is an essential component in the transcriptional process but not in the maintenance of the decondensed state of active chromatin.

scholarly journals Microinjection of anti-topoisomerase I immunoglobulin G into nuclei of Chironomus tentans salivary gland cells leads to blockage of transcription elongation.

1987 ◽  
Vol 7 (12) ◽  
pp. 4308-4316 ◽  
Author(s):  
E Egyházi ◽  
E Durban
Keyword(s):  
Salivary Gland ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Immunoglobulin G ◽  
Topoisomerase I ◽  
Chironomus Tentans ◽  
Balbiani Ring ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Transcriptional Process

Purified anti-topoisomerase I immunoglobulin G (IgG) was microinjected into nuclei of Chironomus tentans salivary gland cells, and the effect on DNA transcription was investigated. Synthesis of nucleolar preribosomal 38S RNA by RNA polymerase I and of chromosomal Balbiani ring RNA by RNA polymerase II was inhibited by about 80%. The inhibitory action of anti-topoisomerase I IgG could be reversed by the addition of exogenous topoisomerase I. Anti-topoisomerase I IgG had less effect on RNA polymerase II-promoted activity of other less efficiently transcribing heterogeneous nuclear RNA genes. The pattern of inhibition of growing nascent Balbiani ring chains indicated that the transcriptional process was interrupted at the level of chain elongation. The highly decondensed state of active Balbiani ring chromatin, however, remained unaffected after injection of topoisomerase I antibodies. These data are consistent with the interpretation that topoisomerase I is an essential component in the transcriptional process but not in the maintenance of the decondensed state of active chromatin.

Balbiani ring RNA content and half-life in nucleus and cytoplasm of Chironomus tentans salivary gland cells

Chromosoma ◽  
1978 ◽  
Vol 66 (1) ◽  
pp. 33-44 ◽  
Author(s):  
J. -E. Edstr�m ◽  
S. Lindgren ◽  
U. L�nn ◽  
L. Rydlander
Keyword(s):  
Salivary Gland ◽  
Half Life ◽  
Chironomus Tentans ◽  
Balbiani Ring ◽  
Gland Cells ◽  
Rna Content ◽  
Salivary Gland Cells

Synthesis of RNA in isolated polytene nuclei from salivary gland cells of Chironomus thummi

1979 ◽  
Vol 124 (1) ◽  
pp. 7-14 ◽  
Author(s):  
R. Mähr ◽  
J.C. Perriard ◽  
M. Lezzi ◽  
H.M. Eppenberger
Keyword(s):  
Salivary Gland ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Polytene Nuclei ◽  
Chironomus Thummi

RNA synthesis in a Balbiani ring in Chironomus tentans salivary gland cells

Chromosoma ◽  
1969 ◽  
Vol 28 (4) ◽  
Author(s):  
B. Daneholt ◽  
J.-E. Edstr�m ◽  
E. Egyh�zi ◽  
B. Lambert ◽  
U. Ringborg
Keyword(s):  
Salivary Gland ◽  
Rna Synthesis ◽  
Chironomus Tentans ◽  
Balbiani Ring ◽  
Gland Cells ◽  
Salivary Gland Cells
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