An interchromosomal gene conversion of the Drosophila dunce locus identified with restriction site polymorphisms: A potential involvement of transposable elements in gene conversion

1987 ◽  
Vol 208 (1-2) ◽  
pp. 315-324 ◽  
Author(s):  
Steven J. Pittler ◽  
Helen K. Salz ◽  
Ronald L. Davis
Keyword(s):  
Transposable Elements ◽  
Gene Conversion ◽  
Restriction Site ◽  
Restriction Site Polymorphisms

Formation of heteroduplex DNA during mammalian intrachromosomal gene conversion

1992 ◽  
Vol 12 (4) ◽  
pp. 1546-1552
Author(s):  
R J Bollag ◽  
D R Elwood ◽  
E D Tobin ◽  
A R Godwin ◽  
R M Liskay
Keyword(s):  
Gene Conversion ◽  
Mammalian Cells ◽  
Restriction Site ◽  
Genetic Evidence ◽  
Heteroduplex Dna ◽  
Daughter Cells ◽  
Single Colony ◽  
Selection For ◽  
Restriction Site Polymorphisms ◽  
Selection Of

We have studied intrachromosomal gene conversion in mouse Ltk- cells with a substrate designed to provide genetic evidence for heteroduplex DNA. Our recombination substrate consists of two defective chicken thymidine kinase genes arranged so as to favor the selection of gene conversion products. The gene intended to serve as the recipient in gene conversion differs from the donor sequence by virtue of a palindromic insertion that creates silent restriction site polymorphisms between the two genes. While selection for gene conversion at a XhoI linker insertion within the recipient gene results in coconversion of the nearby palindromic site in more than half of the convertants, 4% of convertant colonies show both parental and nonparental genotypes at the polymorphic site. We consider these mixed colonies to be the result of genotypic sectoring and interpret this sectoring to be a consequence of unrepaired heteroduplex DNA at the polymorphic palindromic site. DNA replication through the heteroduplex recombination intermediate generates genetically distinct daughter cells that comprise a single colony. We believe that the data provide the first compelling genetic evidence for the presence of heteroduplex DNA during chromosomal gene conversion in mammalian cells.

scholarly journals Decreasing gradients of gene conversion on both sides of the initiation site for meiotic recombination at the ARG4 locus in yeast.

Genetics ◽  
1990 ◽  
Vol 126 (4) ◽  
pp. 813-822 ◽  
Author(s):  
N P Schultes ◽  
J W Szostak
Keyword(s):  
Gene Conversion ◽  
Meiotic Recombination ◽  
Restriction Site ◽  
Initiation Site ◽  
Double Strand Breaks ◽  
Tetrad Analysis ◽  
Strand Breaks ◽  
Second Gradient ◽  
Low Levels ◽  
Restriction Site Polymorphisms

Abstract We have constructed eight restriction site polymorphisms in the DED81-ARG4 region and examined their behavior during meiotic recombination. Tetrad analysis reveals decreasing gradients of gene conversion on both sides of the initiation site for meiotic recombination at the ARG4 locus, extending on one side into the ARG4 gene, and on the other side into the adjacent DED81 gene. Gene conversion events can extend in both directions from the initiation site as the result of a single meiotic event. There is a second gradient of gene conversion in DED81, with high levels near the 5' end of the gene and low levels near the middle of the gene. The peaks of gene conversion activity for the DED81 and ARG4 gradients map to regions where double-strand breaks are found during meiosis. The implications of these results for models of meiotic gene conversion are discussed.

scholarly journals Formation of heteroduplex DNA during mammalian intrachromosomal gene conversion.

1992 ◽  
Vol 12 (4) ◽  
pp. 1546-1552 ◽  
Author(s):  
R J Bollag ◽  
D R Elwood ◽  
E D Tobin ◽  
A R Godwin ◽  
R M Liskay
Keyword(s):  
Gene Conversion ◽  
Mammalian Cells ◽  
Restriction Site ◽  
Genetic Evidence ◽  
Heteroduplex Dna ◽  
Daughter Cells ◽  
Single Colony ◽  
Selection For ◽  
Restriction Site Polymorphisms ◽  
Selection Of

We have studied intrachromosomal gene conversion in mouse Ltk- cells with a substrate designed to provide genetic evidence for heteroduplex DNA. Our recombination substrate consists of two defective chicken thymidine kinase genes arranged so as to favor the selection of gene conversion products. The gene intended to serve as the recipient in gene conversion differs from the donor sequence by virtue of a palindromic insertion that creates silent restriction site polymorphisms between the two genes. While selection for gene conversion at a XhoI linker insertion within the recipient gene results in coconversion of the nearby palindromic site in more than half of the convertants, 4% of convertant colonies show both parental and nonparental genotypes at the polymorphic site. We consider these mixed colonies to be the result of genotypic sectoring and interpret this sectoring to be a consequence of unrepaired heteroduplex DNA at the polymorphic palindromic site. DNA replication through the heteroduplex recombination intermediate generates genetically distinct daughter cells that comprise a single colony. We believe that the data provide the first compelling genetic evidence for the presence of heteroduplex DNA during chromosomal gene conversion in mammalian cells.

scholarly journals Species ofBorreliadistinguished by restriction site polymorphisms in 16S rRNA genes

1993 ◽  
Vol 111 (2-3) ◽  
pp. 239-243 ◽  
Author(s):  
David Ralph ◽  
Daniele Postic ◽  
Guy Baranton ◽  
Charles Pretzman ◽  
Michael McClelland
Keyword(s):  
16S Rrna ◽  
Restriction Site ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Restriction Site Polymorphisms

Expansions and contractions of the genetic map relative to the physical map of yeast chromosome III

1988 ◽  
Vol 8 (2) ◽  
pp. 595-604
Author(s):  
L S Symington ◽  
T D Petes
Keyword(s):  
Gene Conversion ◽  
Restriction Site ◽  
Physical Map ◽  
Minimum Length ◽  
Base Pairs ◽  
Yeast Saccharomyces Cerevisiae ◽  
Yeast Chromosome ◽  
Chromosome Maps ◽  
Chromosome Iii ◽  
The Relationship

To examine the relationship between genetic and physical chromosome maps, we constructed a diploid strain of the yeast Saccharomyces cerevisiae heterozygous for 12 restriction site mutations within a 23-kilobase (5-centimorgan) interval of chromosome III. Crossovers were not uniformly distributed along the chromosome, one interval containing significantly more and one interval significantly fewer crossovers than expected. One-third of these crossovers occurred within 6 kilobases of the centromere. Approximately half of the exchanges were associated with gene conversion events. The minimum length of gene conversion tracts varied from 4 base pairs to more than 12 kilobases, and these tracts were nonuniformly distributed along the chromosome. We conclude that the chromosomal sequence or structure has a dramatic effect on meiotic recombination.

Assignment of two mitochondrially synthesized polypeptides to human mitochondrial DNA and their use in the study of intracellular mitochondrial interaction

1982 ◽  
Vol 2 (1) ◽  
pp. 30-41
Author(s):  
N A Oliver ◽  
D C Wallace
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Site ◽  
Mitochondrial Protein ◽  
Mitochondrial Protein Synthesis ◽  
Human Mitochondrial Dna ◽  
Ht1080 Cells ◽  
Mitochondrial Dnas ◽  
A Cell ◽  
Dna Restriction ◽  
Restriction Site Polymorphisms

Two mitochondrially synthesized marker polypeptides, MV-1 and MV-2, were found in human HeLa and HT1080 cells. These were assigned to the mitochondrial DNA in HeLa-HT1080 cybrids and hybrids by demonstrating their linkage to cytoplasmic genetic markers. These markers include mitochondrial DNA restriction site polymorphisms and resistance to chloramphenicol, an inhibitor of mitochondrial protein synthesis. In the absence of chloramphenicol, the expression of MV-1 and MV-2 in cybrids and hybrids was found to be directly proportional to the ratio of the parental mitochondrial DNAs. In the presence of chloramphenicol, the marker polypeptide linked to the chloramphenicol-sensitive mitochondrial DNA continued to be expressed. This demonstrated that resistant and sensitive mitochondrial DNAs can cooperate within a cell for gene expression and that the CAP-resistant allele was dominant or codominant to sensitive. Such cooperation suggests that mitochondrial DNAs can be exchanged between mitochondria.

Meiotic recombination between repeated transposable elements in Saccharomyces cerevisiae

1988 ◽  
Vol 8 (7) ◽  
pp. 2942-2954
Author(s):  
M Kupiec ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transposable Elements ◽  
Gene Conversion ◽  
Meiotic Recombination ◽  
Small Region ◽  
Ty Elements ◽  
Ty Element ◽  
Reciprocal Exchange ◽  
Gene Conversions ◽  
Conversion Tract

We have measured the frequency of meiotic recombination between marked Ty elements in the Saccharomyces cerevisiae genome. These recombination events were usually nonreciprocal (gene conversions) and sometimes involved nonhomologous chromosomes. The frequency of ectopic gene conversion among Ty elements appeared lower than expected on the basis of previous studies of recombination between artificially constructed repeats. The conversion events involved either a subset of the total Ty elements in the genome or the conversion tract was restricted to a small region of the Ty element. In addition, the observed conversion events were very infrequently associated with reciprocal exchange.

scholarly journals Chromosomal mapping of murine c-fes and c-src genes.

1984 ◽  
Vol 4 (5) ◽  
pp. 978-981 ◽  
Author(s):  
C Blatt ◽  
M E Harper ◽  
G Franchini ◽  
M N Nesbitt ◽  
M I Simon
Keyword(s):  
Kinase Activity ◽  
Restriction Site ◽  
Mouse Genome ◽  
Inbred Strains ◽  
Chromosomal Mapping ◽  
Chromosome 2 ◽  
Distal Half ◽  
Viral Oncogenes ◽  
Tyrosine Specific Kinase ◽  
Restriction Site Polymorphisms

The murine homologs of two viral oncogenes associated with tyrosine-specific kinase activity have been assigned to different loci in the mouse genome. The segregation of restriction site polymorphisms, as detected by probes that are specific for endogenous c-fes and c-src sequences, was followed in the DNA of recombinant inbred strains. The c-fes gene was mapped to the proximal portion of chromosome 7, very close to the Gpi-1 locus, whereas c-src was linked to the Psp locus on the distal half of chromosome 2.

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