Gene cluster for streptomycin biosynthesis in Streptomyces griseus: Analysis of a central region including the major resistance gene

1987 ◽  
Vol 208 (1-2) ◽  
pp. 204-210 ◽  
Author(s):  
Jürgen Distler ◽  
Carola Braun ◽  
Andrea Ebert ◽  
Wolfgang Piepersberg
Keyword(s):  
Gene Cluster ◽  
Resistance Gene ◽  
Central Region ◽  
Streptomyces Griseus ◽  
Streptomycin Biosynthesis ◽  
Major Resistance Gene

scholarly journals The Major Resistance Gene Cluster in Lettuce Is Highly Duplicated and Spans Several Megabases

1998 ◽  
Vol 10 (11) ◽  
pp. 1817 ◽  
Author(s):  
Blake C. Meyers ◽  
Doris B. Chin ◽  
Katherine A. Shen ◽  
Subramoniam Sivaramakrishnan ◽  
Dean O. Lavelle ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Resistance Gene ◽  
Resistance Gene Cluster ◽  
Major Resistance Gene

scholarly journals The Major Resistance Gene Cluster in Lettuce Is Highly Duplicated and Spans Several Megabases

1998 ◽  
Vol 10 (11) ◽  
pp. 1817-1832 ◽  
Author(s):  
Blake C. Meyers ◽  
Doris B. Chin ◽  
Katherine A. Shen ◽  
Subramoniam Sivaramakrishnan ◽  
Dean O. Lavelle ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Resistance Gene ◽  
Resistance Gene Cluster ◽  
Major Resistance Gene

scholarly journals Transcriptional Control by A-Factor of strR, the Pathway-Specific Transcriptional Activator for Streptomycin Biosynthesis in Streptomyces griseus

2005 ◽  
Vol 187 (16) ◽  
pp. 5595-5604 ◽  
Author(s):  
Ayami Tomono ◽  
Yisan Tsai ◽  
Haruka Yamazaki ◽  
Yasuo Ohnishi ◽  
Sueharu Horinouchi
Keyword(s):  
Gene Cluster ◽  
Transcriptional Activation ◽  
Transcriptional Control ◽  
Streptomyces Griseus ◽  
Transcriptional Activator ◽  
Transcriptional Analysis ◽  
Mobility Shift ◽  
Biosynthesis Gene Cluster ◽  
Regulatory Cascade ◽  
Streptomycin Biosynthesis

ABSTRACT A-factor (2-isocapryloyl-3R-hydroxymethyl-γ-butyrolactone) triggers streptomycin production by inducing the transcription of strR, encoding the pathway-specific transcriptional activator, through signal transduction in the A-factor regulatory cascade in Streptomyces griseus. AdpA, one of the key transcriptional activators in the cascade, bound two upstream activation sites, approximately at nucleotide positions −270 and −50 with respect to the transcriptional start point of strR, as determined by gel mobility shift assays and DNase I footprinting. Transcriptional analysis of the strR promoter with mutated AdpA-binding sites showed that both sites were required for full transcriptional activation of strR by AdpA. Potassium permanganate footprinting showed that AdpA assisted RNA polymerase in forming an open complex at an appropriate position for transcriptional initiation of strR. Nine transcriptional units within the streptomycin biosynthesis gene cluster, including the strR-aphD operon, depended on StrR, indicating that StrR is the pathway-specific transcriptional activator for the whole gene cluster. Consistent with this, expression of strR under the control of a constitutively expressed promoter in an adpA null mutant caused the host to produce streptomycin.

scholarly journals Organization and molecular evolution of a disease-resistance gene cluster in coffee trees

BMC Genomics ◽  
2011 ◽  
Vol 12 (1) ◽  
Author(s):  
Alessandra F Ribas ◽  
Alberto Cenci ◽  
Marie-Christine Combes ◽  
Hervé Etienne ◽  
Philippe Lashermes
Keyword(s):  
Disease Resistance ◽  
Molecular Evolution ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Disease Resistance Gene ◽  
Resistance Gene Cluster

scholarly journals Evolution of a Complex Disease Resistance Gene Cluster in Diploid Phaseolus and Tetraploid Glycine

2012 ◽  
Vol 159 (1) ◽  
pp. 336-354 ◽  
Author(s):  
Tom Ashfield ◽  
Ashley N. Egan ◽  
Bernard E. Pfeil ◽  
Nicolas W.G. Chen ◽  
Ram Podicheti ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Complex Disease ◽  
Disease Resistance Gene ◽  
Resistance Gene Cluster

scholarly journals Mapping of a Major Resistance Gene to the Brown Planthopper in the Rice Cultivar Rathu Heenati

2005 ◽  
Vol 55 (4) ◽  
pp. 391-396 ◽  
Author(s):  
Lihong Sun ◽  
Changchao Su ◽  
Chunming Wang ◽  
Huqu Zhai ◽  
Jianmin Wan
Keyword(s):  
Resistance Gene ◽  
Brown Planthopper ◽  
Rice Cultivar ◽  
Major Resistance Gene ◽  
Rathu Heenati

scholarly journals Novel Plasmid-Borne Multidrug Resistance Gene Cluster Includinglsa(E) from a Linezolid-Resistant Enterococcus faecium Isolate of Swine Origin

2015 ◽  
Vol 59 (11) ◽  
pp. 7113-7116 ◽  
Author(s):  
Hongbin Si ◽  
Wan-Jiang Zhang ◽  
Shengbo Chu ◽  
Xiu-Mei Wang ◽  
Lei Dai ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Enterococcus Faecium ◽  
Insertion Element ◽  
Multidrug Resistance Gene ◽  
Content Type ◽  
Recombination Processes ◽  
Faecium Isolate

ABSTRACTA novel nonconjugative plasmid of 28,489 bp from a porcine linezolid-resistantEnterococcus faeciumisolate was completely sequenced. This plasmid harbored a novel type of multiresistance gene cluster that comprised the resistance geneslnu(B),lsa(E),spw,aadE,aphA3, and two copies oferm(B), which account for resistance to macrolides, lincosamides, streptogramins, pleuromutilins, streptomycin, spectinomycin, and kanamycin/neomycin. Structural comparisons suggested that this plasmid might have developed from other enterococcal plasmids by insertion element (IS)-mediated interplasmid recombination processes.

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