Satellite DNA of Drosophila nasuta nasuta and D. n. albomicana: Localization in polytene and metaphase chromosomes

Chromosoma ◽  
1982 ◽  
Vol 85 (3) ◽  
pp. 361-368 ◽  
Author(s):  
H. A. Ranganath ◽  
E. R. Schmidt ◽  
K. H�gele
Keyword(s):  
Satellite Dna ◽  
Metaphase Chromosomes ◽  
Drosophila Nasuta ◽  
Drosophila Nasuta Nasuta

scholarly journals Mouse satellite DNA, centromere structure, and sister chromatid pairing.

1986 ◽  
Vol 103 (4) ◽  
pp. 1145-1151 ◽  
Author(s):  
L M Lica ◽  
S Narayanswami ◽  
B A Hamkalo
Keyword(s):  
Electron Microscopy ◽  
Sister Chromatid ◽  
Satellite Dna ◽  
Marker Chromosome ◽  
Restriction Enzymes ◽  
Centromeric Heterochromatin ◽  
Mitotic Chromosomes ◽  
Metaphase Chromosomes ◽  
Sister Chromatids ◽  
Mouse Satellite Dna

The experiments described were directed toward understanding relationships between mouse satellite DNA, sister chromatid pairing, and centromere function. Electron microscopy of a large mouse L929 marker chromosome shows that each of its multiple constrictions is coincident with a site of sister chromatid contact and the presence of mouse satellite DNA. However, only one of these sites, the central one, possesses kinetochores. This observation suggests either that satellite DNA alone is not sufficient for kinetochore formation or that when one kinetochore forms, other potential sites are suppressed. In the second set of experiments, we show that highly extended chromosomes from Hoechst 33258-treated cells (Hilwig, I., and A. Gropp, 1973, Exp. Cell Res., 81:474-477) lack kinetochores. Kinetochores are not seen in Miller spreads of these chromosomes, and at least one kinetochore antigen is not associated with these chromosomes when they were subjected to immunofluorescent analysis using anti-kinetochore scleroderma serum. These data suggest that kinetochore formation at centromeric heterochromatin may require a higher order chromatin structure which is altered by Hoechst binding. Finally, when metaphase chromosomes are subjected to digestion by restriction enzymes that degrade the bulk of mouse satellite DNA, contact between sister chromatids appears to be disrupted. Electron microscopy of digested chromosomes shows that there is a significant loss of heterochromatin between the sister chromatids at paired sites. In addition, fluorescence microscopy using anti-kinetochore serum reveals a greater inter-kinetochore distance than in controls or chromosomes digested with enzymes that spare satellite. We conclude that the presence of mouse satellite DNA in these regions is necessary for maintenance of contact between the sister chromatids of mouse mitotic chromosomes.

Staining of Satellite DNA in Metaphase Chromosomes

Nature ◽  
1971 ◽  
Vol 231 (5304) ◽  
pp. 532-533 ◽  
Author(s):  
JORGE J. YUNIS ◽  
LILIANA ROLDAN ◽  
WALID G. YASMINEH ◽  
J. C. LEE
Keyword(s):  
Satellite Dna ◽  
Metaphase Chromosomes

scholarly journals Metaphase chromosomes of four species of the Drosophila nasuta subgroup.

1988 ◽  
Vol 63 (5) ◽  
pp. 435-444 ◽  
Author(s):  
Machiko HATSUMI ◽  
Yoichi MORISHIGE ◽  
Ken-Ichi WAKAHAMA
Keyword(s):  
Metaphase Chromosomes ◽  
Drosophila Nasuta

scholarly journals In situ hybridization at the electron microscope level: hybrid detection by autoradiography and colloidal gold

1982 ◽  
Vol 95 (2) ◽  
pp. 609-618 ◽  
Author(s):  
NJ Hutchison ◽  
PR Langer-Safer ◽  
DC Ward ◽  
BA Hamkalo
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscope ◽  
Satellite Dna ◽  
Light Microscope ◽  
Test System ◽  
Microscope Level ◽  
Metaphase Chromosomes ◽  
Light Microscope Level ◽  
Mouse Satellite Dna

In situ hybridization has become a standard method for localizing DNA or RNA sequences in cytological preparations. We developed two methods to extend this technique to the transmission electron microscope level using mouse satellite DNA hybridization to whole mount metaphase chromosomes as the test system. The first method devised is a direct extension of standard light microscope level using mouse satellite DNA hybridization to whole mount metaphase chromosomes as the test system. The first method devised is a direct extension of standard light microscope in situ hybridization. Radioactively labeled complementary RNA (cRNA) is hybridized to metaphase chromosomes deposited on electron microscope grids and fixed in 70 percent ethanol vapor; hybridixation site are detected by autoradiography. Specific and intense labeling of chromosomal centromeric regions is observed even after relatively short exposure times. Inerphase nuclei present in some of the metaphase chromosome preparations also show defined paatterms of satellite DNA labeling which suggests that satellite-containing regions are associate with each other during interphase. The sensitivity of this method is estimated to at least as good as that at the light microscope level while the resolution is improved at least threefold. The second method, which circumvents the use of autoradiogrphic detection, uses biotin-labeled polynucleotide probes. After hybridization of these probes, either DNA or RNA, to fixed chromosomes on grids, hybrids are detected via reaction is improved at least threefold. The second method, which circumvents the use of autoradiographic detection, uses biotin-labeled polynucleotide probes. After hybridization of these probes, either DNA or RNA, to fixed chromosomes on grids, hybrids are detected via reaction with an antibody against biotin and secondary antibody adsorbed to the surface of over centromeric heterochromatin and along the associated peripheral fibers. Labeling is on average ten times that of background binding. This method is rapid and possesses the potential to allow precise ultrastructual localization of DNA sequences in chromosomes and chromatin.

scholarly journals Adaptation to larval crowding in Drosophila ananassae and Drosophila nasuta nasuta: increased larval competitive ability without increased larval feeding rate

2016 ◽  
Vol 95 (2) ◽  
pp. 411-425 ◽  
Author(s):  
ARCHANA NAGARAJAN ◽  
SHARMILA BHARATHI NATARAJAN ◽  
MOHAN JAYARAM ◽  
ANANDA THAMMANNA ◽  
SUDARSHAN CHARI ◽  
...  
Keyword(s):  
Competitive Ability ◽  
Feeding Rate ◽  
Drosophila Ananassae ◽  
Larval Feeding ◽  
Larval Crowding ◽  
Drosophila Nasuta ◽  
Drosophila Nasuta Nasuta
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