Characterization of the lampbrush chromosomes of the marbled newt Triturus marmoratus (Latreille, 1800)

Chromosoma ◽  
1972 ◽  
Vol 37 (1) ◽  
pp. 1-22 ◽  
Author(s):  
Irma Nardi ◽  
Matilde Ragghianti ◽  
Giorgio Mancino
Keyword(s):  
Lampbrush Chromosomes ◽  
Triturus Marmoratus

In situ enzymatic denaturation and random oligonucleotide priming of DNA selectively stains centromeric heterochromatin in Triturus marmoratus (Salamandridae: Caudata)

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 769-771 ◽  
Author(s):  
P. Herrero ◽  
J. L. Bella ◽  
J. de la Torre ◽  
J. Gosálvez
Keyword(s):  
Restriction Endonuclease ◽  
Centromeric Heterochromatin ◽  
Enzymatic Production ◽  
Exonuclease Iii ◽  
Selective Labelling ◽  
Random Priming ◽  
Klenow Polymerase ◽  
Triturus Marmoratus

Enzymatic production of single-stranded DNA with the HaeIII restriction endonuclease and exonuclease III has been used for chromosome labelling involving random primer extension and incorporation of biotinylated nucleotides with the Klenow polymerase on fixed chromosomes of Triturus marmoratus. Results show repetitive and selective labelling of centromeric heterochromatin, while remaining heterochromatin does not exhibit a positive response. This method of chromosome labelling permits the characterization of specific fractions of the heterochromatin and can be used for the analysis of heterochromatin heterogeneity within a given genome.Key words: Triturus marmoratus, cytogenetics, heterochromatin, restriction endonuclease DNA denaturation, random priming.

scholarly journals Characterization of axolotl lampbrush chromosomes by fluorescence in situ hybridization and immunostaining

2021 ◽  
pp. 112523
Author(s):  
Melissa C. Keinath ◽  
Asya Davidian ◽  
Vladimir Timoshevskiy ◽  
Nataliya Timoshevskaya ◽  
Joseph G. Gall
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Lampbrush Chromosomes

Banding patterns on lampbrush chromosomes of Triturus marmoratus (Amphibia Urodela) by the Giemsa stain

Chromosoma ◽  
1974 ◽  
Vol 49 (2) ◽  
pp. 121-134 ◽  
Author(s):  
Renata Batistoni ◽  
Irma Nardi ◽  
Giuseppina Barsacchi Pilone
Keyword(s):  
Giemsa Stain ◽  
Lampbrush Chromosomes ◽  
Banding Patterns ◽  
Triturus Marmoratus

Heterochromatin characterization of sex chromosomes in Triturus marmoratus (Urodela, Salamandridae)

1992 ◽  
Vol 60 (2) ◽  
pp. 150-153 ◽  
Author(s):  
P. Herrero ◽  
J de la Torre ◽  
J. Gosálvez ◽  
A.T. Sumner
Keyword(s):  
Sex Chromosomes ◽  
Triturus Marmoratus

scholarly journals Adaptation of Ranaviruses from Peneda-Gerês National Park (Portugal) to Cell Cultures and their Characterization

2008 ◽  
Vol 14 (S3) ◽  
pp. 139-140 ◽  
Author(s):  
A.P. Alves de Matos ◽  
M.F. Caeiro ◽  
R.E. Marschang ◽  
T. Papp ◽  
C. Soares ◽  
...  
Keyword(s):  
Cell Cultures ◽  
High Mortality ◽  
National Park ◽  
Mass Mortality ◽  
Nw Portugal ◽  
Ultrastructural Characterization ◽  
Triturus Marmoratus

Ranaviruses are known to produce iseases in fish, amphibians and reptiles, being involved in high mortality episodes in aquaculture farms and in the decline of amphibian populations worldwide. Mass mortality episodes were previously detected in Triturus marmoratus from two lagoons of the Peneda-Gerês National Park (PGNP) (NW Portugal). Ranavirus-like particles were identified in tissues of diseased newts T. marmoratus and T. boscai. Here we report on the molecular and ultrastructural characterization of these viruses.

Characterization of nine new microsatellite loci for the marbled newt, Triturus marmoratus

2015 ◽  
Vol 94 (S2) ◽  
pp. 63-64 ◽  
Author(s):  
JEAN-MARC COSTANZI ◽  
PASCAL MÈGE ◽  
SANDRA GUÉRIN ◽  
QUENTIN LE PETITCORPS ◽  
ARTEMIO CARBONELL ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Triturus Marmoratus

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Characterization of Coherent, Ordered Precipitates in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 144-145
Author(s):  
B. H. Kear ◽  
J. M. Oblak
Keyword(s):  
Stable Phase ◽  
Nickel Base Superalloy ◽  
Alloy 718 ◽  
Commercial Alloy ◽  
Precipitate Morphology ◽  
Continuous Precipitation ◽  
Nickel Base ◽  
Base Superalloy ◽  
Strengthening Precipitate

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.

The Use of Advanced Analytical Techniques for Characterization of the Chemical, Physical, and Crystallographic Nature of a Surface

1973 ◽  
Vol 31 ◽  
pp. 132-133 ◽  
Author(s):  
R. E. Herfert
Keyword(s):  
Surface Characterization ◽  
Analytical Techniques ◽  
X Ray Diffraction ◽  
Depth Of Penetration ◽  
X Ray ◽  
The Past ◽  
Transmission Electron ◽  
Scanning Electron

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.

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