Interspecific protoplast fusion to rescue a cytoplasmic lincomycin resistance mutation into fertile Nicotiana plumbaginifolia plants

1984 ◽  
Vol 198 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Ágnes Cséplő ◽  
Ferenc Nagy ◽  
Pál Maliga
Keyword(s):  
Protoplast Fusion ◽  
Resistance Mutation ◽  
Nicotiana Plumbaginifolia ◽  
Lincomycin Resistance

scholarly journals Identification of a Novel Lincomycin Resistance Mutation Associated with Activation of Antibiotic Production in Streptomyces coelicolor A3(2)

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Guojun Wang ◽  
Masumi Izawa ◽  
Xiaoge Yang ◽  
Dongbo Xu ◽  
Yukinori Tanaka ◽  
...  
Keyword(s):  
Novel Mutation ◽  
Antibiotic Production ◽  
Streptomyces Coelicolor ◽  
Resistance Mutation ◽  
Gene Replacement ◽  
Sequencing Analysis ◽  
Hybrid Gene ◽  
Content Type ◽  
High Level ◽  
Lincomycin Resistance

ABSTRACT Comparative genome sequencing analysis of a lincomycin-resistant strain of Streptomyces coelicolor A3(2) and the wild-type strain identified a novel mutation conferring a high level of lincomycin resistance. Surprisingly, the new mutation was an in-frame DNA deletion in the genes SCO4597 and SCO4598, resulting in formation of the hybrid gene linR. SCO4597 and SCO4598 encode two histidine kinases, which together with SCO4596, encoding a response regulator, constitute a unique two-component system. Sequence analysis indicated that these three genes and their arrangement patterns are ubiquitous among all Streptomyces genomes sequenced to date, suggesting these genes play important regulatory roles. Gene replacement showed that this mutation was responsible for the high level of lincomycin resistance, the overproduction of the antibiotic actinorhodin, and the enhanced morphological differentiation of this strain. Moreover, heterologous expression of the hybrid gene linR in Escherichia coli conferred resistance to lincomycin in this organism. Introduction of the hybrid gene linR in various Streptomyces strains by gene engineering technology may widely activate and/or enhance antibiotic production.

scholarly journals EFFECT OF RADIATION DOSAGE ON EFFICIENCY OF CHLOROPLAST TRANSFER BY PROTOPLAST FUSION IN NICOTIANA

Genetics ◽  
1982 ◽  
Vol 100 (3) ◽  
pp. 487-495
Author(s):  
László Menczel ◽  
Gábor Galiba ◽  
Ferenc Nagy ◽  
Pál Maliga
Keyword(s):  
Protoplast Fusion ◽  
Radiation Dosage ◽  
Nicotiana Plumbaginifolia ◽  
Selective Medium ◽  
60Co Source ◽  
Fragmentation Patterns ◽  
Fusion Products ◽  
Lethal Doses ◽  
Nicotiana Tabacum Sr1 ◽  
Ecori Digestion

ABSTRACT Chloroplasts of Nicotiana tabacum SR1 were transferred into Nicotiana plumbaginifolia by protoplast fusion. The protoplasts of the organelle donor were irradiated with different lethal doses using a 60Co source, to facilitate the elimination of their nuclei from the fusion products. After fusion induction, clones derived from fusion products and containing streptomycin-resistant N. tabacum SR1 chloroplasts were selected by their ability to green on a selective medium. When N. tabacum protoplasts were inactivated by iodoacetate instead of irradiation, the proportion of N. plumbaginifolia nuclear segregant clones was low (1-2%). Irradiation markedly increased this value: Using 50, 120, 210 and 300 J kg-1 doses, the frequency of segregant clones was 44, 57, 84 and 70 percent, respectively. Regeneration of resistant N. plumbaginifolia plants with SR1 chloroplasts indicated that plastids can be rescued from the irradiated cells by fusion with untreated protoplasts. Resistant N. plumbaginifolia plants that were regenerated (43 clones studied) had diploid (2n = 2X = 20) or tetraploid chromosome numbers and were identical morphologically to parental plants. The absence of aneuploids suggests that in these clones irradiation resulted in complete elimination of the irradiated N. tabacum nuclei. Resistance is inherited maternally (five clones tested). The demonstration of chloroplast transfer and the presence of N. tabacum plastids in the N. plumbaginifolia plants was confirmed by chloroplast DNA fragmentation patterns after EcoRI digestion.

Artificial Hybridization of Ganoderma lucidum and G. tsugae Murrill by Protoplast Fusion for Sustainability

2005 ◽  
Vol 7 (1-2) ◽  
pp. 263-280 ◽  
Author(s):  
Siu Wai Chiu ◽  
Vivien Wing Yan Luk ◽  
Stephen Yu ◽  
Peggy Lee ◽  
Natalie Wai ◽  
...  
Keyword(s):  
Protoplast Fusion ◽  
Ganoderma Lucidum ◽  
Artificial Hybridization

Extracellular polysaccharides from suspension cultures of Nicotiana plumbaginifolia

2020 ◽  
Author(s):  
Ian Sims ◽  
A Bacic
Keyword(s):  
Uronic Acid ◽  
Cell Suspension Cultures ◽  
Anion Exchange Chromatography ◽  
Nicotiana Plumbaginifolia ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Selective Precipitation ◽  
The Individual

The soluble polymers secreted by cell-suspension cultures of Nicotiana plumbaginifolia contained 78% carbohydrate, 6% protein and 4% inorganic material. The extracellular polysaccharides were separated into three fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7 and the individual polysaccharides in each fraction were then isolated by selective precipitation and enzymic treatment. Monosaccharide and linkage compositions were determined for each polysaccharide after reduction of uronic acid residues and the degree of esterification of the various uronic acid residues in each polysaccharide was determined concurrently with the linkage types. Six components were identified: an arabinoxyloglucan (comprising 34% of the total polysaccharide) and a galactoglucomannan (15%) in the unbound neutral fraction, a type II arabinogalactan (an arabinogalactan-protein, 11%) and an acidic xylan (3%) in the first bound fraction, and an arabinoglucuronomannan (11%) and a galacturonan (26%) in the second bound fraction. © 1995.

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