Combination of kanamycin resistance and nitrate reductase deficiency as selectable markers in one nuclear genome provides a universal somatic hybridizer in plants

1987 ◽  
Vol 208 (3) ◽  
pp. 469-473 ◽  
Author(s):  
Christian Brunold ◽  
Susanne Krüger-Lebus ◽  
Michael W. Saul ◽  
Samuel Wegmüller ◽  
Igo Potrykus
Keyword(s):  
Nitrate Reductase ◽  
Nuclear Genome ◽  
Kanamycin Resistance ◽  
Selectable Markers ◽  
Reductase Deficiency ◽  
Nitrate Reductase Deficiency

Effect of nitrate reductase deficiency on the accumulation of phenylpropanoids in Arabidopsis thaliana leaves

2009 ◽  
Vol 153 (2) ◽  
pp. S216-S217
Author(s):  
Halley C. Oliveira ◽  
Plinio R. Santos Filho ◽  
Pablo G. Ferreira ◽  
Renan C. Chisté ◽  
Adriana Z. Mercadante ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Nitrate Reductase ◽  
Reductase Deficiency ◽  
Nitrate Reductase Deficiency

M2 seed screening for nitrate reductase deficiency in Nicotiana plumbaginifolia

Plant Science ◽  
1991 ◽  
Vol 76 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Frédérique Pelsy ◽  
Jocelyne Kronenberger ◽  
Jean-Marie Pollien ◽  
Michel Caboche
Keyword(s):  
Nitrate Reductase ◽  
Nicotiana Plumbaginifolia ◽  
Reductase Deficiency ◽  
Nitrate Reductase Deficiency

Nitrate Reductase Genes as Selectable Markers for Plant Cell Transformation

1983 ◽  
pp. 215-231 ◽  
Author(s):  
A. Kleinhofs ◽  
J. Taylor ◽  
T. M. Kuo ◽  
D. A. Somers ◽  
R. L. Warner
Keyword(s):  
Nitrate Reductase ◽  
Plant Cell ◽  
Cell Transformation ◽  
Selectable Markers ◽  
Plant Cell Transformation

scholarly journals Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase.

1989 ◽  
Vol 109 (6) ◽  
pp. 2589-2601 ◽  
Author(s):  
K L Kindle ◽  
R A Schnell ◽  
E Fernández ◽  
P A Lefebvre
Keyword(s):  
Enzyme Activity ◽  
Nitrate Reductase ◽  
Plasmid Dna ◽  
Reductase Activity ◽  
Nuclear Genome ◽  
Nuclear Transformation ◽  
Wild Type ◽  
Gene Encoding ◽  
Photosynthetic Organism ◽  
Low Efficiency

We have developed a nuclear transformation system for Chlamydomonas reinhardtii, using micro-projectile bombardment to introduce the gene encoding nitrate reductase into a nit1 mutant strain which lacks nitrate reductase activity. By using either supercoiled or linear plasmid DNA, transformants were recovered consistently at a low efficiency, on the order of 15 transformants per microgram of plasmid DNA. In all cases the transforming DNA was integrated into the nuclear genome, usually in multiple copies. Most of the introduced copies were genetically linked to each other, and they were unlinked to the original nit1 locus. The transforming DNA and nit+ phenotype were stable through mitosis and meiosis, even in the absence of selection. nit1 transcripts of various sizes were expressed at levels equal to or greater than those in wild-type nit+ strains. In most transformants, nitrate reductase enzyme activity was expressed at approximately wild-type levels. In all transformants, nit1 mRNA and nitrate reductase enzyme activity were repressed in cells grown on ammonium medium, showing that expression of the integrated nit1 genes was regulated normally. When a second plasmid with a nonselectable gene was bombarded into the cells along with the nit1 gene, transformants carrying DNA from both plasmids were recovered. In some cases, expression of the unselected gene could be detected. With the advent of nuclear transformation in Chlamydomonas, it becomes the first photosynthetic organism in which both the nuclear and chloroplast compartments can be transformed.

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