Analysis of the heterogeneity within bovine pineal gland by immunohistochemistry and in situ hybridization

1994 ◽  
Vol 277 (2) ◽  
pp. 201-209 ◽  
Author(s):  
Tetsuji Sato ◽  
Michinari Kaneko ◽  
Hiroki Fujieda ◽  
Takeo Deguchi ◽  
Kenjiro Wake
Keyword(s):  
In Situ Hybridization ◽  
Pineal Gland ◽  
Bovine Pineal Gland

Analysis of the heterogeneity within bovine pineal gland by immunohistochemistry and in situ hybridization

1994 ◽  
Vol 277 (2) ◽  
pp. 201-209 ◽  
Author(s):  
Tetsuji Sato ◽  
Michinari Kaneko ◽  
Hiroki Fujieda ◽  
Takeo Deguchi ◽  
Kenjiro Wake
Keyword(s):  
In Situ Hybridization ◽  
Pineal Gland ◽  
Bovine Pineal Gland

Presence of oxytocinergic neuronal-like cells in the bovine pineal gland: an immunocytochemical and in situ hybridization study

2001 ◽  
Vol 31 (3) ◽  
pp. 273-280 ◽  
Author(s):  
Corin Badiu ◽  
Liliana Badiu ◽  
Mihail Coculescu ◽  
Hans Vilhardt ◽  
Morten Moller
Keyword(s):  
In Situ Hybridization ◽  
Pineal Gland ◽  
Hybridization Study ◽  
Bovine Pineal Gland

Cloning and widespread distribution of the rat rod-type cyclic nucleotide-gated cation channel

1997 ◽  
Vol 272 (4) ◽  
pp. C1335-C1344 ◽  
Author(s):  
C. Ding ◽  
E. D. Potter ◽  
W. Qiu ◽  
S. L. Coon ◽  
M. A. Levine ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Pineal Gland ◽  
Northern Blot ◽  
Blot Analysis ◽  
Cyclic Nucleotide ◽  
Northern Blot Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

We used Northern blot analysis, ribonuclease protection assay (RPA), reverse transcriptase-polymerase chain reaction, and in situ hybridization to investigate the hypothesis that the CNG1 isoform of the cyclic nucleotide-gated nonselective cation channel may be widely distributed in tissues of the rat. A cDNA encoding the CNG1 isoform was isolated from rat eye and human retina, and partial sequences were isolated from rat pineal gland and human kidney. Northern blot analysis revealed a 3.1-kilobase (kb) CNG1 transcript in rat eye, pineal gland, pituitary, adrenal gland, and spleen, and a larger transcript of 3.5 kb was found in testis. RPA confirmed the identity of CNG1 mRNA in rat eye, lung, spleen, and brain. Polymerase chain reaction-based detection of the mRNA for CNG1 indicates that the channel is expressed in lower abundance in many other tissues, including thymus, skeletal muscle, heart, and parathyroid gland. The cellular distribution of CNG1 was further studied by in situ hybridization, which demonstrated expression of mRNA in lung, thymus, pineal gland, hippocampus, cerebellum, and cerebral cortex but not in heart or kidney.

Developmental expression of tryptophan hydroxylase mRNAs in the rat pineal gland: an in situ hybridization study

1995 ◽  
Vol 29 (2) ◽  
pp. 253-262 ◽  
Author(s):  
Roger Besançon ◽  
Latifa Chouaf ◽  
Anne Jouvet ◽  
Sigismond Sliwinski ◽  
Marie-Françoise Belin ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Pineal Gland ◽  
Tryptophan Hydroxylase ◽  
Developmental Expression ◽  
Hybridization Study ◽  
Rat Pineal Gland

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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