Ribosomal genes of Neurospora crassa: Constancy of gene number in the conidial and mycelial phases, and homogeneity in length and restriction enzyme cleavage sites within strains

1983 ◽  
Vol 192 (1-2) ◽  
pp. 285-287 ◽  
Author(s):  
Karin D. Rodland ◽  
Peter J. Russell
Keyword(s):  
Neurospora Crassa ◽  
Restriction Enzyme ◽  
Ribosomal Genes ◽  
Cleavage Sites ◽  
Gene Number ◽  
Enzyme Cleavage

Mapping of additional restriction enzyme cleavage sites on bacteriophage MB78 genome

1998 ◽  
Vol 23 (2) ◽  
pp. 151-154 ◽  
Author(s):  
S. S. Murty ◽  
B. Pandey ◽  
Maharani Chakravorty
Keyword(s):  
Restriction Enzyme ◽  
Additional Restriction ◽  
Cleavage Sites ◽  
Bacteriophage Mb78 ◽  
Enzyme Cleavage

scholarly journals Novel Approach to Mapping of Resistance Mutations in Whole Genomes by Using Restriction Enzyme Modulation of Transformation Efficiency

2005 ◽  
Vol 49 (7) ◽  
pp. 2767-2777 ◽  
Author(s):  
Claude G. Lerner ◽  
Stephan J. Kakavas ◽  
Christian Wagner ◽  
Richard T. Chang ◽  
Philip J. Merta ◽  
...  
Keyword(s):  
Restriction Enzyme ◽  
Genomic Dna ◽  
Efflux Pump ◽  
Resistance Mutation ◽  
Resistance Locus ◽  
Cleavage Sites ◽  
Resistance Mutations ◽  
Mapping Procedure ◽  
Enzyme Modulation ◽  
Enzyme Cleavage

ABSTRACT Restriction enzyme modulation of transformation efficiencies (REMOTE) is a method that makes use of genome restriction maps and experimentally observed differences in transformation efficiencies of genomic DNA restriction digests to discover the location of mutations in genomes. The frequency with which digested genomic DNA from a resistant strain transforms a susceptible strain to resistance is primarily determined by the size of the fragment containing the resistance mutation and the distance of the mutation to the end of the fragment. The positions of restriction enzyme cleavage sites immediately flanking the resistance mutation define these parameters. The mapping procedure involves a process of elimination in which digests that transform with high frequency indicate that the restriction enzyme cleavage sites are relatively far away from the mutation, while digests that transform with low frequency indicate that the sites are close to the mutation. The transformation data are compared computationally to the genome restriction map to identify the regions that best fit the data. Transformations with PCR amplicons encompassing candidate regions identify the resistance locus and enable identification of the mutation. REMOTE was developed using Haemophilus influenzae strains with mutations in gyrA, gyrB, and rpsE that confer resistance to ciprofloxacin, novobiocin, and spectinomycin, respectively. We applied REMOTE to identify mutations that confer resistance to two novel antibacterial compounds. The resistance mutations were found in genes that can decrease the intracellular concentration of compounds: acrB, which encodes a subunit of the AcrAB-TolC efflux pump; and fadL, which encodes a long-chain fatty acid transporter.

scholarly journals A restriction enzyme cleavage map of the histidine utilization (hut) genes of Klebsiella aerogenes and deletions lacking regions of hut DNA

1984 ◽  
Vol 193 (1) ◽  
pp. 92-98 ◽  
Author(s):  
S. A. Boylan ◽  
Linda J. Eades ◽  
Kaaren A. Janssen ◽  
Margaret I. Lomax ◽  
R. A. Bender
Keyword(s):  
Restriction Enzyme ◽  
Klebsiella Aerogenes ◽  
Enzyme Cleavage

Optimising restriction enzyme cleavage of DNA derived from archival histopathological samples: an improved HUMARA assay

Pathology ◽  
2003 ◽  
Vol 35 (1) ◽  
pp. 70-74
Author(s):  
Lidija Jovanovic ◽  
Brett Delahunt ◽  
Bryan McIver ◽  
Norman L. Eberhardt ◽  
Stefan K. G. Grebe
Keyword(s):  
Restriction Enzyme ◽  
Enzyme Cleavage ◽  
Humara Assay

Optimising restriction enzyme cleavage of DNA derived from archival histopathological samples: an improved HUMARA assay

Pathology ◽  
2003 ◽  
Vol 35 (1) ◽  
pp. 70-74 ◽  
Author(s):  
Lidija Jovanovic ◽  
Brett Delahunt ◽  
Bryan McIver ◽  
Norman L. Eberhardt ◽  
Stefan K.G. Grebe
Keyword(s):  
Restriction Enzyme ◽  
Enzyme Cleavage ◽  
Humara Assay
Sign in / Sign up

Export Citation Format

Share Document