Primary structure of the trpC gene from Aspergillus nidulans

1985 ◽  
Vol 199 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Edward J. Mullaney ◽  
John E. Hamer ◽  
Kellee A. Roberti ◽  
M. Melanie Yelton ◽  
William E. Timberlake
Keyword(s):  
Aspergillus Nidulans ◽  
Primary Structure ◽  
Trpc Gene

scholarly journals Developmental regulation of the Aspergillus nidulans trpC gene.

1983 ◽  
Vol 80 (24) ◽  
pp. 7576-7580 ◽  
Author(s):  
M. M. Yelton ◽  
J. E. Hamer ◽  
E. R. de Souza ◽  
E. J. Mullaney ◽  
W. E. Timberlake
Keyword(s):  
Aspergillus Nidulans ◽  
Developmental Regulation ◽  
Trpc Gene

Functional organization of the Aspergillus nidulans trpC promoter

1987 ◽  
Vol 7 (7) ◽  
pp. 2352-2359
Author(s):  
J E Hamer ◽  
W E Timberlake
Keyword(s):  
Gene Expression ◽  
Aspergillus Nidulans ◽  
Functional Organization ◽  
Fusion Gene ◽  
Lacz Gene ◽  
Galactosidase Activity ◽  
S1 Nuclease ◽  
Promoter Mutations ◽  
Beta Galactosidase ◽  
Trpc Gene

We investigated the functional organization of the Aspergillus nidulans trpC promoter by the sequential removal of sequences upstream of the major trpC mRNA cap site (+1). DNA fragments containing promoter mutations were fused to the Escherichia coli lacZ gene, and a novel method was used to select for integration of the fusion gene at the Aspergillus argB locus. beta-Galactosidase assays and S1 nuclease protection experiments demonstrated that the promoter mutations affected gene expression in three ways: (i) 5' deletions up to -82 resulted in variable increases in beta-galactosidase activity, depending on the growth conditions; (ii) a deletion from -67 to -11 did not alter the level of beta-galactosidase activity, but did give rise to mRNAs with aberrant 5' ends; and (iii) a 5' deletion with an endpoint at -11 and an internal deletion from -142 to -11 abolished gene expression. These results indicate that sequences upstream of -82 reduce transcription of the trpC gene and that distinct DNA sequence elements are required for expression versus correct initiation of transcription of the trpC gene. The sequences essential for trpC expression do not include the common eucaryotic promoter elements CCAAT and TATAAA. To our knowledge, this is the first functional analysis of a promoter from a fungus other than Saccharomyces cerevisiae.

scholarly journals Purification, properties and primary structure of thioredoxin from Aspergillus nidulans

1992 ◽  
Vol 210 (2) ◽  
pp. 421-429 ◽  
Author(s):  
Pierre MARECHAL ◽  
Boi Minh Chau HOANG ◽  
Jean-Marie SCHMITTER ◽  
Alain DORSSELAER ◽  
Paulette DECOTTIGNIES
Keyword(s):  
Aspergillus Nidulans ◽  
Primary Structure

scholarly journals Functional organization of the Aspergillus nidulans trpC promoter.

1987 ◽  
Vol 7 (7) ◽  
pp. 2352-2359 ◽  
Author(s):  
J E Hamer ◽  
W E Timberlake
Keyword(s):  
Gene Expression ◽  
Aspergillus Nidulans ◽  
Functional Organization ◽  
Fusion Gene ◽  
Lacz Gene ◽  
Galactosidase Activity ◽  
S1 Nuclease ◽  
Promoter Mutations ◽  
Beta Galactosidase ◽  
Trpc Gene

We investigated the functional organization of the Aspergillus nidulans trpC promoter by the sequential removal of sequences upstream of the major trpC mRNA cap site (+1). DNA fragments containing promoter mutations were fused to the Escherichia coli lacZ gene, and a novel method was used to select for integration of the fusion gene at the Aspergillus argB locus. beta-Galactosidase assays and S1 nuclease protection experiments demonstrated that the promoter mutations affected gene expression in three ways: (i) 5' deletions up to -82 resulted in variable increases in beta-galactosidase activity, depending on the growth conditions; (ii) a deletion from -67 to -11 did not alter the level of beta-galactosidase activity, but did give rise to mRNAs with aberrant 5' ends; and (iii) a 5' deletion with an endpoint at -11 and an internal deletion from -142 to -11 abolished gene expression. These results indicate that sequences upstream of -82 reduce transcription of the trpC gene and that distinct DNA sequence elements are required for expression versus correct initiation of transcription of the trpC gene. The sequences essential for trpC expression do not include the common eucaryotic promoter elements CCAAT and TATAAA. To our knowledge, this is the first functional analysis of a promoter from a fungus other than Saccharomyces cerevisiae.

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