Excision of copia element in a revertant of the white-apricot mutation of Drosophila melanogaster leaves behind one long-terminal repeat

1985 ◽  
Vol 199 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Bruno Dalle Carbonare ◽  
Walter J. Gehring
Keyword(s):  
Drosophila Melanogaster ◽  
Long Terminal Repeat ◽  
Terminal Repeat ◽  
Copia Element

scholarly journals The 5′ Untranslated Region and Gag product of Idefix, a Long Terminal Repeat-Retrotransposon from Drosophila melanogaster, Act Together To Initiate a Switch between Translated and Untranslated States of the Genomic mRNA

2003 ◽  
Vol 23 (22) ◽  
pp. 8246-8254 ◽  
Author(s):  
Carine Meignin ◽  
Jean-Luc Bailly ◽  
Frédérick Arnaud ◽  
Bernard Dastugue ◽  
Chantal Vaury
Keyword(s):  
Drosophila Melanogaster ◽  
Long Terminal Repeat ◽  
Untranslated Region ◽  
Terminal Repeat ◽  
Entry Site ◽  
Independent Manner ◽  
Gag Protein ◽  
Genomic Arrangement

ABSTRACT Idefix is a long terminal repeat (LTR)-retrotransposon present in Drosophila melanogaster which shares similarities with vertebrates retroviruses both in its genomic arrangement and in the mechanism of transposition. Like in retroviruses, its two LTRs flank a long 5′ untranslated region (5′UTR) and three open reading frames referred to as the gag, pol, and env genes. Here we report that its 5′UTR, located upstream of the gag gene, can fold into highly structured domains that are known to be incompatible with efficient translation by ribosome scanning. Using dicistronic plasmids analyzed by both (i) in vitro transcription and translation in rabbit reticulocyte or wheat germ lysates and (ii) in vivo expression in transgenic flies, we show that the 5′UTR of Idefix exhibits an internal ribosome entry site (IRES) activity that is able to promote translation of a downstream cistron in a cap-independent manner. The functional state of this novel IRES depends on eukaryotic factors that are independent of their host origin. However, in vivo, its function can be down-regulated by trans-acting factors specific to tissues or developmental stages of its host. We identify one of these trans-acting factors as the Gag protein encoded by Idefix itself. Our data support a model in which nascent Gag is able to block translation initiated from the viral mRNA and thus its own translation. These data highlight the fact that LTR-retrotransposons may autoregulate their replication cycle through their Gag production.

scholarly journals Behavior of a Drosophila melanogaster transposable element in Saccharomyces cerevisiae

1985 ◽  
Vol 5 (11) ◽  
pp. 3325-3329
Author(s):  
D K Hoshizaki ◽  
D J Finnegan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drosophila Melanogaster ◽  
Homologous Recombination ◽  
Long Terminal Repeat ◽  
Transposable Element ◽  
Gene Product ◽  
Terminal Repeat ◽  
Long Terminal Repeats ◽  
Terminal Repeats ◽  
412 Element

The Drosophila melanogaster transposable element 412 is transiently unstable in Saccharomyces cerevisiae when present on a freely replicating plasmid. The 412 element undergoes recombination to form two circular molecules, a 412 deletion plasmid and, presumably, a 412 circle. The 412 deletion plasmid contains a single long terminal repeat which most likely is the result of homologous recombination within the long terminal repeats. This recombination occurs at or shortly after transformation and is independent of both the RAD52 gene product and the Flp gene of 2 micron DNA.

scholarly journals Behavior of a Drosophila melanogaster transposable element in Saccharomyces cerevisiae.

1985 ◽  
Vol 5 (11) ◽  
pp. 3325-3329 ◽  
Author(s):  
D K Hoshizaki ◽  
D J Finnegan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drosophila Melanogaster ◽  
Homologous Recombination ◽  
Long Terminal Repeat ◽  
Transposable Element ◽  
Gene Product ◽  
Terminal Repeat ◽  
Long Terminal Repeats ◽  
Terminal Repeats ◽  
412 Element

The Drosophila melanogaster transposable element 412 is transiently unstable in Saccharomyces cerevisiae when present on a freely replicating plasmid. The 412 element undergoes recombination to form two circular molecules, a 412 deletion plasmid and, presumably, a 412 circle. The 412 deletion plasmid contains a single long terminal repeat which most likely is the result of homologous recombination within the long terminal repeats. This recombination occurs at or shortly after transformation and is independent of both the RAD52 gene product and the Flp gene of 2 micron DNA.

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