Restriction enzyme analysis of a highly diverged satellite DNA from Drosophila nasutoides

Chromosoma ◽  
1981 ◽  
Vol 83 (3) ◽  
pp. 367-379 ◽  
Author(s):  
C. S. Lee
Keyword(s):  
Restriction Enzyme ◽  
Satellite Dna ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis

Restriction Enzyme Analysis of Satellite DNA Components from the Bovine Genome

1978 ◽  
Vol 84 (1) ◽  
pp. 189-195 ◽  
Author(s):  
Helena KOPECKA ◽  
Gabriel MACAYA ◽  
Jordi CORTADAS ◽  
Jean-Paul THIERY ◽  
Giorgio BERNARDI
Keyword(s):  
Restriction Enzyme ◽  
Satellite Dna ◽  
Bovine Genome ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Dna Components

Long interspersed repeated DNA (LINE) causes polymorphism at the rat insulin 1 locus

1985 ◽  
Vol 5 (9) ◽  
pp. 2197-2203
Author(s):  
M S Lakshmikumaran ◽  
E D'Ambrosio ◽  
L A Laimins ◽  
D T Lin ◽  
A V Furano
Keyword(s):  
Dna Sequence ◽  
Restriction Enzyme ◽  
Allelic Variation ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Repeated Dna ◽  
Coding Region ◽  
Sequence Determination ◽  
Polymorphic Region ◽  
Or Gene

The insulin 1, but not the insulin 2, locus is polymorphic (i.e., exhibits allelic variation) in rats. Restriction enzyme analysis and hybridization studies showed that the polymorphic region is 2.2 kilobases upstream of the insulin 1 coding region and is due to the presence or absence of an approximately 2.7-kilobase repeated DNA element. DNA sequence determination showed that this DNA element is a member of a long interspersed repeated DNA family (LINE) that is highly repeated (greater than 50,000 copies) and highly transcribed in the rat. Although the presence or absence of LINE sequences at the insulin 1 locus occurs in both the homozygous and heterozygous states, LINE-containing insulin 1 alleles are more prevalent in the rat population than are alleles without LINEs. Restriction enzyme analysis of the LINE-containing alleles indicated that at least two versions of the LINE sequence may be present at the insulin 1 locus in different rats. Either repeated transposition of LINE sequences or gene conversion between the resident insulin 1 LINE and other sequences in the genome are possible explanations for this.

Restriction Enzyme Analysis (REA) of Group A Streptococcal (GAS) M-Serotypes 1, 3, and 28

1997 ◽  
pp. 221-223 ◽  
Author(s):  
Dwight R. Johnson ◽  
Cheryl L. Romana ◽  
Carey D. Rehder ◽  
Joanne Dehnbostel ◽  
Edward L. Kaplan
Keyword(s):  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Group A

scholarly journals Identificação de micobactérias não tuberculosas isoladas de sítios estéreis em pacientes em um hospital universitário na cidade do Rio de Janeiro

2011 ◽  
Vol 37 (4) ◽  
pp. 521-526 ◽  
Author(s):  
Simone Gonçalves Senna ◽  
Ana Grazia Marsico ◽  
Gisele Betzler de Oliveira Vieira ◽  
Luciana Fonseca Sobral ◽  
Philip Noel Suffys ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Restriction Enzyme ◽  
Rio De Janeiro ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis

OBJETIVO: Identificar micobactérias não tuberculosas (MNT) isoladas de sítios estéreis em pacientes internados no Hospital Universitário Clementino Fraga Filho, Rio de Janeiro (RJ) entre 2001 e 2006. MÉTODOS: Durante o período do estudo, 34 isolados de MNT de sítios estéreis de 14 pacientes, a maioria HIV positivos, foram submetidos a identificação fenotípica e hsp65 PCR-restriction enzyme analysis (PRA, análise por enzimas de restrição por PCR do gene hsp65). RESULTADOS: A maioria dos isolados foi identificada como Mycobacterium avium, seguida por M. monacense, M. kansasii e M. abscessus em menores proporções. CONCLUSÕES: A combinação de PRA, um método relativamente simples e de baixo custo, com algumas características fenotípicas pode fornecer a identificação correta de MNT na rotina de laboratórios clínicos.

RESTRICTION ENZYME ANALYSIS OF HERPESVIRUS-2 DNA

The Lancet ◽  
1981 ◽  
Vol 318 (8260-8261) ◽  
pp. 1424 ◽  
Author(s):  
IsabelW Smith ◽  
N.J Maitland ◽  
J.F Peutherer ◽  
D.H.H Robertson
Keyword(s):  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis
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