A new insertion element, ISH26, from Halobacterium halobium

1987 ◽  
Vol 206 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Klaus Ebert ◽  
Christian Hanke ◽  
Hajo Delius ◽  
Werner Goebel ◽  
Felicitas Pfeifer
1989 ◽  
Vol 35 (1) ◽  
pp. 96-100 ◽  
Author(s):  
Felicitas Pfeifer ◽  
Ulrike Blaseio ◽  
Mary Horne

Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10−2, while the chromosomal gene expressed in the stationary phase of growth is mutated with a frequency of 10−5. The difference in the mutation susceptibility is due to the dynamics of plasmid pHH1. p-vac gene mutations are caused (i) by the integration of an insertion element or (ii) by a deletion event encompassing the p-vac gene region. In contrast, c-vac mutants analyzed to date incurred neither insertion elements nor deletions. Deletion events within pHH1 occur at high frequencies during the development of a H. halobium culture. The investigation of the fusion regions resulting from deletion events indicates that insertion elements are involved. The analysis of pHH1 deletion variants led to a 4 kilobase pair DNA region containing the origin of replication of the pHH1 plasmid.Key words: gas vacuole protein gene, plasmid dynamics, deletions, insertion elements.


2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity ◽  
Dorothea Taylor

1984 ◽  
Vol 5 (1) ◽  
pp. 1-8 ◽  
Author(s):  
AKIRA TONOSAKI ◽  
FUMIO TOKUNAGA ◽  
HIROSHI WASHIOKA ◽  
KATAOKA MIKIO ◽  
OSAMU HISATOMI

1983 ◽  
Vol 258 (13) ◽  
pp. 7964-7967
Author(s):  
J Krupinski ◽  
J L Spudich ◽  
G G Hammes

FEBS Letters ◽  
1978 ◽  
Vol 91 (1) ◽  
pp. 131-134 ◽  
Author(s):  
David Cahen ◽  
Haim Garty ◽  
S.Roy Caplan

1986 ◽  
Vol 50 (5) ◽  
pp. 895-900 ◽  
Author(s):  
S.A. Sundberg ◽  
M. Alam ◽  
J.L. Spudich

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