Combination of micellar extraction of polycyclic aromatic hydrocarbons from aqueous media with detection by synchronous fluorescence

1993 ◽  
Vol 346 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Achim B�ckelen ◽  
Reinhard Niessner
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Aqueous Media ◽  
Synchronous Fluorescence ◽  
Micellar Extraction ◽  
Polycyclic Aromatic

Degradation of polycyclic aromatic hydrocarbons (PAHs) by a mixed culture and its component pure cultures, obtained from PAH-contaminated soil

1995 ◽  
Vol 41 (6) ◽  
pp. 470-476 ◽  
Author(s):  
D. Trzesicka-Mlynarz ◽  
O. P. Ward
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Mixed Culture ◽  
Aromatic Hydrocarbons ◽  
Aqueous Media ◽  
Water Soluble ◽  
Nitrogen Levels ◽  
Glucose Levels ◽  
Polycyclic Aromatic ◽  
Pure Cultures ◽  
The Media

A mixed culture, isolated from soil contaminated with polycyclic aromatic hydrocarbons (PAHs), grew on and degraded fluoranthene in aqueous media supplemented with glucose, yeast extract, and peptone. Increased complex nitrogen levels in the medium promoted bacterial growth and a greater extent of fluoranthene degradation. Amendment of the media with high glucose levels also diminished specific fluoranthene degradation. The mixed culture was capable of degrading a range of other PAHs, including benzo[a]pyrene, anthracene, phenanthrene, acenaphthene, and fluorene. The mixed culture contained four predominant isolates, all of which were Gram-negative rods, three of which were identified as Pseudomonas putida, Flavobacterium sp., and Pseudomonas aeruginosa. Better degradation of a defined PAH mixture was observed with the mixed culture than with individual isolates. A reconstituted culture, prepared by combining the four individual isolates, manifested a similar PAH biodegradation performance to the original mixed culture. When compared with the mixed culture, individual isolates exhibited a relatively good capacity to remove more water-soluble PAHs (acenaphthene, fluorene, phenanthrene, fluoranthene). In contrast, removal of less water-soluble PAHs (anthracene and pyrene) was low or negligible with isolated cultures compared with the mixed culture.Key words: polycyclic aromatic hydrocarbons, mixed culture, fluoranthene, Pseudomonas, Flavobacterium.

Ultrasonic micellar extraction of polycyclic aromatic hydrocarbons from marine sediments

Talanta ◽  
2001 ◽  
Vol 54 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Verónica Pino ◽  
Juan H. Ayala ◽  
Ana M. Afonso ◽  
Venerando González
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Marine Sediments ◽  
Aromatic Hydrocarbons ◽  
Micellar Extraction ◽  
Polycyclic Aromatic
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