?Phase variation?-type regulation of gene expression and gene replacement mediated by FLP recombinase in the yeast Saccharomyces cerevisiae

1993 ◽  
Vol 24 (1-2) ◽  
pp. 26-31 ◽  
Author(s):  
Sergei V. Saveliev ◽  
Michael Yu. Fessing ◽  
Alexei M. Kopylov ◽  
Gleb I. Kirjanov
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Regulation Of Gene Expression ◽  
Phase Variation ◽  
Gene Replacement ◽  
Yeast Saccharomyces Cerevisiae ◽  
Flp Recombinase

Plant-Derived Transcription Factors for Orthologous Regulation of Gene Expression in the Yeast Saccharomyces cerevisiae

2017 ◽  
Vol 6 (9) ◽  
pp. 1742-1756 ◽  
Author(s):  
Gita Naseri ◽  
Salma Balazadeh ◽  
Fabian Machens ◽  
Iman Kamranfar ◽  
Katrin Messerschmidt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Transcription Factors ◽  
Regulation Of Gene Expression ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals Post-Transcriptional Control of Mating-Type Gene Expression during Gametogenesis in Saccharomyces cerevisiae

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1223
Author(s):  
Randi Yeager ◽  
G. Guy Bushkin ◽  
Emily Singer ◽  
Rui Fu ◽  
Benjamin Cooperman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Transcriptional Control ◽  
Regulation Of Gene Expression ◽  
Mating Types ◽  
Yeast Saccharomyces Cerevisiae ◽  
Type Gene ◽  
Diploid Cells ◽  
Retained Introns

Gametogenesis in diploid cells of the budding yeast Saccharomyces cerevisiae produces four haploid meiotic products called spores. Spores are dormant until nutrients trigger germination, when they bud asexually or mate to return to the diploid state. Each sporulating diploid produces a mix of spores of two haploid mating types, a and α. In asexually dividing haploids, the mating types result from distinct, mutually exclusive gene expression programs responsible for production of mating pheromones and the receptors to sense them, all of which are silent in diploids. It was assumed that spores only transcribe haploid- and mating-type-specific genes upon germination. We find that dormant spores of each mating type harbor transcripts representing all these genes, with the exception of Mata1, which we found to be enriched in a spores. Mata1 transcripts, from a rare yeast gene with two introns, were mostly unspliced. If the retained introns reflect tethering to the MATa locus, this could provide a mechanism for biased inheritance. Translation of pheromones and receptors were repressed at least until germination. We find antisense transcripts to many mating genes that may be responsible. These findings add to the growing number of examples of post-transcriptional regulation of gene expression during gametogenesis.

scholarly journals Noncoding RNA participation in gene expression regulation in yeast Saccharomyces cerevisiae

2011 ◽  
Vol 9 (1) ◽  
pp. 3-14
Author(s):  
Olga V Kochenova
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Gene Regulation ◽  
Antisense Rna ◽  
Noncoding Rna ◽  
Gene Expression Regulation ◽  
Regulation Of Gene Expression ◽  
Yeast Genome ◽  
Yeast Saccharomyces Cerevisiae ◽  
Main Components

Saccharomyces cerevisiae lacks the main components of RNAi-dependent gene silencing. Nevertheless, regulation of gene expression in S. cerevisiae could be accomplished via some other types of noncoding RNA, particularly via antisense RNA. Although, there is a high percent of untranslated RNA in yeast genome only few evidences of noncoding RNA gene regulation exist in yeast S. cerevisiae, some of them are reviewed in the present paper. 

Positive control of sporulation-specific genes by the IME1 and IME2 products in Saccharomyces cerevisiae

1990 ◽  
Vol 10 (5) ◽  
pp. 2104-2110
Author(s):  
A P Mitchell ◽  
S E Driscoll ◽  
H E Smith
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Positive Control ◽  
Spore Formation ◽  
Specific Gene ◽  
Heterologous Promoter ◽  
Yeast Saccharomyces Cerevisiae ◽  
Specific Gene Expression ◽  
Rapid Induction

In the yeast Saccharomyces cerevisiae, meiosis and spore formation require the induction of sporulation-specific genes. Two genes are thought to activate the sporulation program: IME1 and IME2 (inducer of meiosis). Both genes are induced upon entry into meiosis, and IME1 is required for IME2 expression. We report here that IME1 is essential for expression of four sporulation-specific genes. In contrast, IME2 is not absolutely essential for expression of the sporulation-specific genes, but contributes to their rapid induction. Expression of IME2 from a heterologous promoter permits the expression of these sporulation-specific genes, meiotic recombination, and spore formation in the absence of IME1. We propose that the IME1 and IME2 products can each activate sporulation-specific genes independently. In addition, the IME1 product stimulates sporulation-specific gene expression indirectly through activation of IME2 expression.

Molecular genetic analysis of Saccharomyces cerevisiae C1-tetrahydrofolate synthase mutants reveals a noncatalytic function of the ADE3 gene product and an additional folate-dependent enzyme

1990 ◽  
Vol 10 (11) ◽  
pp. 5679-5687
Author(s):  
C K Barlowe ◽  
D R Appling
Keyword(s):  
Saccharomyces Cerevisiae ◽  
De Novo ◽  
Point Mutations ◽  
Gene Replacement ◽  
Molecular Genetic Analysis ◽  
Yeast Cells ◽  
Yeast Saccharomyces Cerevisiae ◽  
Purine Synthesis

In eucaryotes, 10-formyltetrahydrofolate (formyl-THF) synthetase, 5,10-methenyl-THF cyclohydrolase, and NADP(+)-dependent 5,10-methylene-THF dehydrogenase activities are present on a single polypeptide termed C1-THF synthase. This trifunctional enzyme, encoded by the ADE3 gene in the yeast Saccharomyces cerevisiae, is thought to be responsible for the synthesis of the one-carbon donor 10-formyl-THF for de novo purine synthesis. Deletion of the ADE3 gene causes adenine auxotrophy, presumably as a result of the lack of cytoplasmic 10-formyl-THF. In this report, defined point mutations that affected one or more of the catalytic activities of yeast C1-THF synthase were generated in vitro and transferred to the chromosomal ADE3 locus by gene replacement. In contrast to ADE3 deletions, point mutations that inactivated all three activities of C1-THF synthase did not result in an adenine requirement. Heterologous expression of the Clostridium acidiurici gene encoding a monofunctional 10-formyl-THF synthetase in an ade3 deletion strain did not restore growth in the absence of adenine, even though the monofunctional synthetase was catalytically competent in vivo. These results indicate that adequate cytoplasmic 10-formyl-THF can be produced by an enzyme(s) other than C1-THF synthase, but efficient utilization of that 10-formyl-THF for purine synthesis requires a nonenzymatic function of C1-THF synthase. A monofunctional 5,10-methylene-THF dehydrogenase, dependent on NAD+ for catalysis, has been identified and purified from yeast cells (C. K. Barlowe and D. R. Appling, Biochemistry 29:7089-7094, 1990). We propose that the characteristics of strains expressing full-length but catalytically inactive C1-THF synthase could result from the formation of a purine-synthesizing multienzyme complex involving the structurally unchanged C1-THF synthase and that production of the necessary one-carbon units in these strains is accomplished by an NAD+ -dependent 5,10-methylene-THF dehydrogenase.

scholarly journals Role of IME1 expression in regulation of meiosis in Saccharomyces cerevisiae.

1990 ◽  
Vol 10 (12) ◽  
pp. 6103-6113 ◽  
Author(s):  
H E Smith ◽  
S S Su ◽  
L Neigeborn ◽  
S E Driscoll ◽  
A P Mitchell
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Specific Gene ◽  
Alpha Cells ◽  
Cell Type ◽  
Yeast Saccharomyces Cerevisiae ◽  
Specific Gene Expression ◽  
Gal1 Promoter ◽  
Acid Protein

Two signals are required for meiosis and spore formation in the yeast Saccharomyces cerevisiae: starvation and the MAT products a1 and alpha 2, which determine the a/alpha cell type. These signals lead to increased expression of the IME1 (inducer of meiosis) gene, which is required for sporulation and sporulation-specific gene expression. We report here the sequence of the IME1 gene and the consequences of IME1 expression from the GAL1 promoter. The deduced IME1 product is a 360-amino-acid protein with a tyrosine-rich C-terminal region. Expression of PGAL1-IME1 in vegetative a/alpha cells led to moderate accumulation of four early sporulation-specific transcripts (IME2, SPO11, SPO13, and HOP1); the transcripts accumulated 3- to 10-fold more after starvation. Two sporulation-specific transcripts normally expressed later (SPS1 and SPS2) did not accumulate until PGAL1-IME1 strains were starved, and the intact IME1 gene was not activated by PGAL1-IME1 expression. In a or alpha cells, which lack alpha 2 or a1, expression of PGAL1-IME1 led to the same pattern of IME2 and SPO13 expression as in a/alpha cells, as measured with ime2::lacZ and spo13::lacZ fusions. Thus, in wild-type strains, the increased expression of IME1 in starved a/alpha cells can account entirely for cell type control, but only partially for nutritional control, of early sporulation-specific gene expression. PGAL1-IME1 expression did not cause growing cells to sporulate but permitted efficient sporulation of amino acid-limited cells, which otherwise sporulated poorly. We suggest that IME1 acts primarily as a positive regulator of early sporulation-specific genes and that growth arrest is an independent prerequisite for execution of the sporulation program.

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