Detection of particulate polycyclic aromatic hydrocarbons by laser-induced time-resolved fluorescence

1991 ◽  
Vol 341 (3-4) ◽  
pp. 207-213 ◽  
Author(s):  
Reinhard Niessner ◽  
Wilfried Robers ◽  
Andreas Krupp
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Polycyclic Aromatic

Application of multi-way models to the time-resolved fluorescence of polycyclic aromatic hydrocarbons mixtures in water

2004 ◽  
Vol 38 (9) ◽  
pp. 2269-2276 ◽  
Author(s):  
Elena Selli ◽  
Cristina Zaccaria ◽  
Fabrizio Sena ◽  
Giorgio Tomasi ◽  
Giovanni Bidoglio
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Polycyclic Aromatic

Fluorometry of Carcinogenic Polycyclic Aromatic Hydrocarbons in Biological Systems

1993 ◽  
Vol 47 (10) ◽  
pp. 1604-1608 ◽  
Author(s):  
Ruixia Song ◽  
Kuang-Pang Li
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Oxygen Saturation ◽  
Aromatic Hydrocarbons ◽  
Biological Systems ◽  
Heme Protein ◽  
Single Band ◽  
Protein Ratio ◽  
Time Resolved ◽  
Polycyclic Aromatic ◽  
Cytochrome P 450

Benzo(a)pyrene (BP) in liposomes and microsomes exhibits overlapping excimer bands peaked at 495 and 520 nm, respectively, in contrast to the single band of pyrene at 480 nm. Since BP is asymmetrical and pyrene is symmetrical, the dual maxima imply that the BP molecules in the excimer must be oriented either parallel or anti-parallel to each other. Time-resolved studies of BP oxidation by cytochrome P-450 show that the 520-nm component dissipates much faster than the 495-nm component during the first 30 min of metabolism, indicating that the initial P-450-BP interaction may be BP orientation sensitive. The binding of BP to the porphyrin group of a heme protein (e.g., hemoglobin or myoglobin) appears to be stronger under oxygen saturation than under CO2 saturation conditions. The transfer of BP between the membrane and the porphyrin group, as measured by means of excimer/monomer ratios, appears to be significantly different from that of pyrene. As the liposome-to-protein ratio increases, the excimer/monomer of BP steadily decreases, whereas that of pyrene increases and reaches a plateau. The significance of this phenomenon in their mechanisms of metabolism will have to be investigated further.

Sign in / Sign up

Export Citation Format

Share Document