Transcript analysis of penicillin genes from Penicillium chrysogenum

1992 ◽  
Vol 21 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Didier V. Renno ◽  
Gunter Saunders ◽  
Alan T. Bull ◽  
Geoffrey Holt
Keyword(s):  
Penicillium Chrysogenum ◽  
Transcript Analysis ◽  
Penicillin Genes

Properties and Biotechnological Application of mutant Derivatives of Mini-intein PRP8 from Penicillium chrysogenum with Improved Control of C-terminal Processing

2019 ◽  
Vol 35 (6) ◽  
pp. 91-101
Author(s):  
F.A. Klebanov ◽  
S.E. Cheperegin ◽  
D.G. Kozlov
Keyword(s):  
Penicillium Chrysogenum ◽  
Protein Denaturation ◽  
Biologically Active ◽  
Cultivation Temperature ◽  
Target Proteins ◽  
E Coli ◽  
Complete Inactivation ◽  
Target Molecules ◽  
Proteins And Peptides

Mutant variants of mini-intein PRP8 from Penicillium chrysogenum (Int4b) with improved control of C-terminal processing were characterized. The presented variants can serve as a basis for self-removed polypeptide tags capable of carrying an affine label and allowing to optimize the process of obtaining target proteins and peptides in E. coli cells. They allow to synthesize target molecules in the composition of soluble and insoluble hybrid proteins (fusions), provide their afnne purification, autocatalytic processing and obtaining mature target products. The presented variants have a number of features in comparison with the known prototypes. In particular the mutant mini-intein Int4bPRO, containing the L93P mutation, has temperature-dependent properties. At cultivation temperature below 30 °C it allows the production of target molecules as part of soluble fusions, but after increasing of cultivation temperature to 37 °C it directs the most of synthesized fusions into insoluble intracellular aggregates. The transition of Int4bPRO into insoluble form is accompanied by complete inactivation of C-terminal processing. Further application of standard protein denaturation-renaturation procedures enable efficiently reactivate Int4bPRO and to carry out processing of its fusions in vitro. Two other variants, Int4b56 and Int4b36, containing a point mutation T62N or combination of mutations D144N and L146T respectively, have a reduced rate of C-terminal processing. Their use in E. coli cells allows to optimize the biosynthesis of biologically active target proteins and peptides in the composition of soluble fusions, suitable for afnne purification and subsequent intein-dependent processing without the use of protein denaturation-renaturation procedures. intein, fusion, processing, processing rate, gelonin The work was supported within the framework of the State Assignment no. 595-00003-19 PR.

Mitochondrial RNA Transcript Analysis Assay of Arabidopsis Leaf Tissues

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (20) ◽  
Author(s):  
Etienne Delannoy ◽  
And�ol Falcon de Longevialle ◽  
Catherine Francs-Small
Keyword(s):  
Mitochondrial Rna ◽  
Transcript Analysis ◽  
Leaf Tissues ◽  
Rna Transcript

scholarly journals Isolation and characterization of the acetyl-CoA synthetase from Penicillium chrysogenum. Involvement of this enzyme in the biosynthesis of penicillins.

1992 ◽  
Vol 267 (8) ◽  
pp. 5474-5481
Author(s):  
H Martínez-Blanco ◽  
A Reglero ◽  
M Fernández-Valverde ◽  
M.A. Ferrero ◽  
M.A. Moreno ◽  
...  
Keyword(s):  
Penicillium Chrysogenum ◽  
Acetyl Coa ◽  
Isolation And Characterization

scholarly journals Characterization of the SOS response of Pseudomonas fluorescens strain DC206 using whole-genome transcript analysis

2007 ◽  
Vol 269 (2) ◽  
pp. 256-264 ◽  
Author(s):  
Hongfan Jin ◽  
Diane M. Retallack ◽  
Steven J. Stelman ◽  
C. Douglas Hershberger ◽  
Tom Ramseier
Keyword(s):  
Pseudomonas Fluorescens ◽  
Sos Response ◽  
Whole Genome ◽  
Transcript Analysis

Separation of Chitosan from Penicillium Chrysogenum Mycelium and its Applications

2002 ◽  
Vol 17 (3) ◽  
pp. 173-182 ◽  
Author(s):  
Tan Tianwei ◽  
Wang Binwu ◽  
Shi Xinyuan
Keyword(s):  
Penicillium Chrysogenum

scholarly journals Sulphur-containing amino acids and peptides in the mycelium of Penicillium chrysogenum

1960 ◽  
Vol 76 (2) ◽  
pp. 353-357 ◽  
Author(s):  
H. R. V. Arnstein ◽  
M. Artman ◽  
D. Morris ◽  
E. J. Toms
Keyword(s):  
Amino Acids ◽  
Penicillium Chrysogenum
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