The identification of 18 nuclear genes required for the expression of the yeast mitochondrial gene encoding cytochrome c oxidase subunit 1

1992 ◽  
Vol 21 (2) ◽  
pp. 139-146 ◽  
Author(s):  
H. J. Pel ◽  
A. Tzagoloff ◽  
L. A. Grivell
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Mitochondrial Gene ◽  
Nuclear Genes ◽  
Gene Encoding ◽  
Oxidase Subunit

scholarly journals Deletion of the Leader Peptide of the Mitochondrially Encoded Precursor of Saccharomyces cerevisiae Cytochrome c Oxidase Subunit II

Genetics ◽  
1997 ◽  
Vol 145 (4) ◽  
pp. 903-910 ◽  
Author(s):  
A Thomas Torello ◽  
Michael H Overholtzer ◽  
Vicki L Cameron ◽  
Nathalie Bonnefoy ◽  
Thomas D Fox
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Cytochrome B ◽  
Mitochondrial Gene ◽  
Leader Peptide ◽  
Intermembrane Space ◽  
Gene Encoding ◽  
Amino Terminal ◽  
Oxidase Subunit

Cytochrome c oxidase subunit II (Cox2p) of Saccharomyces cerevisiae is synthesized within mitochondria as a precursor, pre-Cox2p. The 15-amino acid leader peptide is processed after export to the intermembrane space. Leader peptides are relatively unusual in mitochondrially coded proteins: indeed mammalian Cox2p lacks a leader peptide. We generated two deletions in the S. cerevisiae COX2 gene, removing either the leader peptide (cox2-20) or the leader peptide and processing site (cox2-21) without altering either the promoter or the mRNA-specific translational activation site. When inserted into mtDNA, both deletions substantially reduced the steady-state levels of Cox2p and caused a tight nonrespiratory phenotype. A respiring pseudorevertant of the cox2-20 mutant was heteroplasmic for the original mutant mtDNA and a ρ–-mtDNA whose deletion fused the first 251 codons of the mitochondrial gene encoding cytochrome b to the cox2-20 sequence. The resulting fusion protein was processed to yield functional Cox2p. Thus, the presence of amino-terminal cytochrome b sequence bypassed the need for the pre-Cox2p leader peptide. We propose that the pre-Cox2p leader peptide contains a targeting signal necessary for membrane insertion, without which it remains in the matrix and is rapidly degraded.

Isostenosmylus ammirabilis sp. nov., a remarkable new species of lance lacewing (Neuroptera: Osmylidae) from the Colombian Andes

Zootaxa ◽  
2020 ◽  
Vol 4803 (3) ◽  
pp. 561-575
Author(s):  
ADRIAN ARDILA-CAMACHO ◽  
CALEB CALIFRE MARTINS ◽  
JORGE ARI NORIEGA
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Mitochondrial Gene ◽  
Dna Barcode ◽  
Neotropical Region ◽  
Andean Region ◽  
Oxidase Subunit ◽  
Colombian Andes ◽  
South Of Brazil

Isostenosmylus Krüger, 1913 is the richest genus of Osmylidae of the Neotropical region, with 17 described species so far, which are distributed mainly in the Andean region and in the South of Brazil and Paraguay. A new remarkable Colombian species of Isostenosmylus—I. ammirabilis sp. nov.—is herein described and illustrated. DNA barcode of mitochondrial gene cytochrome c oxidase subunit I (COI) for this species is also provided. Taxonomic keys for the genus are updated. 

scholarly journals Molecular characterization of human isolates of Strongyloides stercoralis and Rhabditis spp. based on mitochondrial cytochrome c oxidase subunit 1 (cox1)

2019 ◽  
Author(s):  
Mandana Fadaei Tehrani ◽  
Meysam Sharifdini ◽  
Farzaneh Zahabiun ◽  
Robabeh Latifi ◽  
Eshrat bigom Kia
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Molecular Characterization ◽  
Mitochondrial Gene ◽  
Pcr Amplification ◽  
Strongyloides Stercoralis ◽  
Cox1 Gene ◽  
Oxidase Subunit ◽  
Stool Samples ◽  
Fresh Stool

Abstract Background: In microscopical examinations of stool samples, S. stercoralis may be miss-diagnosed with other nematodes infecting human digestive system, especially Rhabditis species that release larvae in stool samples. The aim of this study was molecular characterization and differentiation of human derived isolates of S. stercoralis and Rhabditis species based on the mitochondrial gene of cytochrome c oxidase subunit 1 (cox1) amplification. Methods: Using parasitological methods, ten isolates of S. stercoralis and three isolates of Rhabditis spp. were obtained from fresh stool samples of patients and the genomic DNA of the samples were extracted. PCR amplification of cox1 gene was carried out for all the isolates and the products were sequenced. Results: The phylogenetic analysis illustrated that S. stercoralis and Rhabditis spp. isolates were placed in two distinguishable separate clades. Inter-species genetic variation between isolates of S. stercoralis and Rhabditis spp. were ranged from 13.5 to 14.5%. Conclusions: Cox1 gene was a suitable marker for discrimination of S. stercoralis from Rhabditis spp. retrieved from human in the current study.

A review of the genus Saccharicoccus Ferris, 1950 (Hemiptera: Coccomorpha: Pseudococcidae) in China, with description of a new species

Zootaxa ◽  
2018 ◽  
Vol 4375 (1) ◽  
pp. 127
Author(s):  
JIANG-TAO ZHANG ◽  
BO-WEN WU ◽  
SAN-AN WU
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Host Plants ◽  
Additional Data ◽  
Mitochondrial Gene ◽  
Molecular Analyses ◽  
Oxidase Subunit ◽  
Adult Females ◽  
A New Species

The mealybug genus Saccharicoccus Ferris includes three species: S. isfarensis (Borchsenius, 1949), S. sacchari (Cockerell, 1895) and a new species, S. chinensis Zhang, Wu & Wu sp. n. The new species, collected on Miscanthus sp. (Poaceae) and other poaceous weeds in China, is described and illustrated, and molecular analyses based on the mitochondrial gene cytochrome c oxidase subunit I (COI) are provided. Additional data on the distribution and host-plants of each species are given, and a key to the adult females of Saccharicoccus species is provided. 

scholarly journals Molecular characterization of human isolates of Strongyloides stercoralis and Rhabditis spp. based on mitochondrial cytochrome c oxidase subunit 1 (cox1)

2019 ◽  
Author(s):  
Mandana Fadaei Tehrani ◽  
Meysam Sharifdini ◽  
Farzaneh Zahabiun ◽  
Robabeh Latifi ◽  
Eshrat bigom Kia
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Molecular Characterization ◽  
Mitochondrial Gene ◽  
Strongyloides Stercoralis ◽  
Sequence Information ◽  
Cox1 Gene ◽  
Microscopical Examination ◽  
Oxidase Subunit ◽  
Stool Samples

Abstract Abstract Background: Due to the similarity of Strongyloides stercoralis with free-living nematodes of Rhabditis species they might be miss-diagnosed with each other in microscopical examination of stool samples. The aim of this study was molecular characterization and differentiation of human derived isolates of S. stercoralis and Rhabditis species based on the mitochondrial gene of cytochrome c oxidase subunit 1 ( cox1 ) amplification. Methods: Using parasitological methods, ten isolates of S. stercoralis and three isolates of Rhabditis spp. were obtained from fresh stool samples of patients and the genomic DNA of the samples were extracted. PCR amplification of cox1 gene was carried out for all the isolates and the products were sequenced. Results: The phylogenetic analysis illustrated that S. stercoralis and Rhabditis spp. isolates were placed in two distinguishable separate clades. Inter-species genetic variation between isolates of S. stercoralis and Rhabditis spp . were ranged from 13.5 to 14.5%. Conclusions: Cox1 gene was a suitable marker for discrimination of S. stercoralis from Rhabditis spp. retrieved from human in the current study. The availability of gene sequence information will be helpful in the future development and validation of discriminatory PCR-based assays of these nematodes.

scholarly journals Structure of the promoter region of the gene encoding cytochrome c oxidase subunit V in Dictyostelium

1993 ◽  
Vol 211 (3) ◽  
pp. 411-414 ◽  
Author(s):  
Rosario RIZZUTO ◽  
Dorianna SANDONA ◽  
Marisa BRINI ◽  
Rolf MARSCHALEK ◽  
Theodor DINGERMANN ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Promoter Region ◽  
Gene Encoding ◽  
Oxidase Subunit

scholarly journals The Relationships within the Poecilimon ornatus Group (Orthoptera: Phaneropterinae) Based on the Cytochrome C Oxidase I Gene

2020 ◽  
Vol 68 (1) ◽  
pp. 7-13
Author(s):  
Maciej Kociński
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Mitochondrial Gene ◽  
Morphological Characteristics ◽  
Cytochrome C Oxidase I ◽  
Oxidase Subunit ◽  
Distribution And Ecology ◽  
Bush Crickets ◽  
I Gene

The genus Poecilimon includes 142 species divided into 18 groups. It is distributed throughout the Palaearctic area. One of the groups is the Poecilimon ornatus group, in which many closely related taxa have been identified (13 species). Although several searches have been carried out, the phylogeny and systematics of P. ornatus are only partly resolved. The most dispersed taxon within the group is Poecilimon affinis, having numerous subspecies. Species from the P. ornatus group have been described mainly based on morphological characteristics, as well as type of song. The aim of this study is to clarify the relationships between species from the P. ornatus group by comparing partial sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene. The analyses were carried out on 84 specimens from 23 taxa. Bush-crickets from the P. ornatus group are monophyletic, in contrast to taxa within the P. affinis complex. Not all of the previously described divisions of the group based on morphology, bioacoustics, distribution, and ecology were confirmed.

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