Blood vessels in the tongue of the kitten: Scanning electron microscopy of microcorrosion casts

1979 ◽  
Vol 155 (3) ◽  
pp. 347-353 ◽  
Author(s):  
A. Jasiński ◽  
A. Miodoński
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Vessels ◽  
Microcorrosion Casts ◽  
Scanning Electron

scholarly journals Three-dimensional relationships between tumor cells and microcirculation with double cyanine immunolabeling, laser scanning confocal microscopy, and computer-assisted reconstruction: an alternative to cast corrosion preparations.

1994 ◽  
Vol 42 (5) ◽  
pp. 681-686 ◽  
Author(s):  
V Rummelt ◽  
L M Gardner ◽  
R Folberg ◽  
S Beck ◽  
B Knosp ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Vessels ◽  
Tumor Cells ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Melanoma Cells ◽  
Tumor Blood Vessels ◽  
The Relationship ◽  
Scanning Electron

The morphology of the microcirculation of uveal melanomas is a reliable market of tumor progression. Scanning electron microscopy of cast corrosion preparations can generate three-dimensional views of these vascular patterns, but this technique sacrifices the tumor parenchyma. Formalin-fixed wet tissue sections 100-150 microns thick from uveal melanomas were stained with the lectin Ulex europaeus agglutinin I (UEAI) and proliferating cell nuclear antigen (PCNA) to demonstrate simultaneously the tumor blood vessels and proliferating tumor cells. Indocarbocyanine (Cy3) was used as a fluorophore for UEAI and indodicarbocyanine (Cy5) was used for PCNA. Double labeled sections were examined with a laser scanning confocal microscope. Images of both stains were digitized at the same 5-microns intervals and each of the two images per interval was combined digitally to form one image. These combined images were visualized through voxel processing to study the relationship between melanoma cells expressing PCNA and various microcirculatory patterns. This technique produces images comparable to scanning electron microscopy of cast corrosion preparations while permitting simultaneous localization of melanoma cells expressing PCNA. The microcirculatory tree can be viewed from any perspective and the relationship between tumor cells and the tumor blood vessels can be studied concurrently in three dimensions. This technique is an alternative to cast corrosion preparations.

Light and Scanning Electron Microscopy of Human Spinal Ligaments

1985 ◽  
Vol 55 ◽  
Author(s):  
L.-H. Yahia ◽  
G. Drouin ◽  
C.-H. Rivard
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Connective Tissue ◽  
Idiopathic Scoliosis ◽  
Blood Vessels ◽  
Structural Changes ◽  
Loose Connective Tissue ◽  
Morphological Studies ◽  
Spinal Ligaments ◽  
Scanning Electron

ABSTRACTSpinal ligaments were obtained from normal and scoliotic individuals. Detailed morphological studies were carried out on the yellow, interspinous and supraspinous ligaments by light and scanning electron microscopy. Normal yellow ligaments are mostly constituted of dense elastin fibers with only a few collagen fibers and sparse blood vessels, while in normal interspinous and supraspinous ligaments, the presence of collagen is highly dominant. In the latter structures, the collagen fascicles are characterized by a regular waviness morphology. The fibrils constituting the fascicles appear either parallel or helical with respect to the fascicle axis. Structural changes are observed in the spinal ligaments of patients with congenital as well as idiopathic scoliosis. For yellow ligaments, only slight differences are found between normal and scoliotic specimens. However, alterations in collagen waviness and architecture are observed mainly in the supraspinous ligaments and to a lesser extent in the interspinous ligaments. In addition, increases in the cellularity, loose connective tissue and vessels are found in both forgoing ligaments. These results indicate that the more pronounced scoliosis-related changes occur in ligaments having the farthest distance from the axis of flexion-rotation.

Lower extremity peripheral vascular disease. Part 1: Scanning electron microscopy

1989 ◽  
Vol 79 (11) ◽  
pp. 527-535
Author(s):  
DJ McCarthy ◽  
N Abell ◽  
T Reed
Keyword(s):  
Electron Microscopy ◽  
At Risk ◽  
Scanning Electron Microscopy ◽  
Lower Extremity ◽  
Vascular Disease ◽  
Blood Vessels ◽  
Peripheral Vascular Disease ◽  
Peripheral Vascular ◽  
Tissue Alterations ◽  
Scanning Electron

The surgical loss of the foot or leg is vigorously resisted by podiatrists who are committed to the conservation of limbs at risk because of peripheral vascular disease. Pathologic changes in blood vessels may, however, progress to a point where no other option is available to the patient. This study assesses amputation of the extremities and investigates tissue alterations that can be identified in diseased blood vessels implicated in these circumstances. Gross pathology and scanning electron microscopy are examined in this, the first of a two-part study.

Morphological Aspects of the Traumatic Chronic Subdural Hematoma Capsule: SEM Studies

2007 ◽  
Vol 13 (3) ◽  
pp. 211-219 ◽  
Author(s):  
Marek Moskała ◽  
Igor Gościński ◽  
Józef Kałuża ◽  
Jarosław Polak ◽  
Mariusz Krupa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Vessels ◽  
Morphological Changes ◽  
Chronic Subdural Hematoma ◽  
Dynamic Changes ◽  
Inflammatory Reactions ◽  
Morphological Aspects ◽  
Thin Walled ◽  
Scanning Electron

The morphology of the outer and inner membranes of traumatic chronic subdural hematomas (CSDHs) surgically removed from eight patients was investigated by scanning electron microscopy (SEM). Hematomas were divided into three groups based on time that had passed from the initiation of trauma to surgery. Structure of the CSDHs showed gradual morphological changes of the developing hematoma capsule. They initially included angiogenic and aseptic inflammatory reactions followed by progressive involvement of fibroblasts—proliferating and producing collagen fibrils. Numerous capillaries suggesting formation of new blood vessels were observed mainly in young hematomas removed between 15 and 21 days after trauma. In “older” hematomas (40 days after trauma), more numerous capillaries and thin-walled sinusoids were accompanied by patent, larger diameter blood vessels. Within the fibrotic outer membrane of the “oldest” hematoma capsules (60 or more days after trauma), especially in the area over the hematoma cavity, blood vessels were frequently occluded by clots. The results suggest dynamic changes in cellular and vascular organization of traumatic CSDH capsules paralleling the progression in hematoma age.

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