A novel method for in situ screening of yeast colonies with the ?-glucuronidase reporter gene

1991 ◽  
Vol 20 (5) ◽  
pp. 437-439 ◽  
Author(s):  
Heribert Hirt
Keyword(s):  
Reporter Gene ◽  
Glucuronidase Reporter ◽  
Novel Method

scholarly journals Development of a novel method for the in-situ dechlorination of immovable iron elements: optimization of Cl− extraction yield through experimental design

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marco Veneranda ◽  
Nagore Prieto-Taboada ◽  
Jose Antonio Carrero ◽  
Ilaria Costantini ◽  
Aitor Larrañaga ◽  
...  
Keyword(s):  
Experimental Design ◽  
Optimization Design ◽  
Degradation Process ◽  
Extraction Yield ◽  
Alkaline Solutions ◽  
Iron Core ◽  
Total Consumption ◽  
Iron Leaching ◽  
Novel Method

AbstractThe conservation of iron objects exposed to marine aerosol is threatened by the formation of akaganeite, a highly unstable Cl-bearing corrosion phase. As akaganeite formation is responsible of the exfoliation of the rust layer, chlorides trigger a cyclic alteration phenomenon that often ends with the total consumption of the iron core. To prevent this degradation process, movable iron elements (e.g. archaeometallurgical artefacts) are generally immersed in alkaline dechlorination baths. Aiming to transfer this successful method to the treatment of immovable iron objects, we propose the in-situ application of alkaline solutions through the use of highly absorbent wraps. As first step of this novel research line, the present work defines the best desalination solution to be used and optimizes its extraction yield. After literature review, a screening experimental design was performed to understand the single and synergic effects of common additives used for NaOH baths. Once the most effective variables were selected, an optimization design was carried out to determine the optimal conditions to be set during treatment. According to the experimental work here presented, the use of 0.7 M NaOH solutions applied at high temperatures (above 50 °C) is recommended. Indeed, these conditions enhance chloride extraction and iron leaching inhibition, while promoting corrosion stabilization.

scholarly journals Using Sentinel-2 Images to Estimate Topography, Tidal-Stage Lags and Exposure Periods over Large Intertidal Areas

2021 ◽  
Vol 13 (2) ◽  
pp. 320
Author(s):  
José P. Granadeiro ◽  
João Belo ◽  
Mohamed Henriques ◽  
João Catalão ◽  
Teresa Catry
Keyword(s):  
Large Scale ◽  
Exposure Period ◽  
Intertidal Area ◽  
Digital Elevation ◽  
Geographical Bias ◽  
Novel Method ◽  
Tidal Stage ◽  
Sentinel 2

Intertidal areas provide key ecosystem services but are declining worldwide. Digital elevation models (DEMs) are important tools to monitor the evolution of such areas. In this study, we aim at (i) estimating the intertidal topography based on an established pixel-wise algorithm, from Sentinel-2 MultiSpectral Instrument scenes, (ii) implementing a set of procedures to improve the quality of such estimation, and (iii) estimating the exposure period of the intertidal area of the Bijagós Archipelago, Guinea-Bissau. We first propose a four-parameter logistic regression to estimate intertidal topography. Afterwards, we develop a novel method to estimate tide-stage lags in the area covered by a Sentinel-2 scene to correct for geographical bias in topographic estimation resulting from differences in water height within each image. Our method searches for the minimum differences in height estimates obtained from rising and ebbing tides separately, enabling the estimation of cotidal lines. Tidal-stage differences estimated closely matched those published by official authorities. We re-estimated pixel heights from which we produced a model of intertidal exposure period. We obtained a high correlation between predicted and in-situ measurements of exposure period. We highlight the importance of remote sensing to deliver large-scale intertidal DEM and tide-stage data, with relevance for coastal safety, ecology and biodiversity conservation.

A novel method for in situ stabilization of calcium arsenic residues via yukonite formation

2022 ◽  
pp. 153090
Author(s):  
Yumeng Wang ◽  
Shaofeng Wang ◽  
Yu Song ◽  
Peiwen Zhang ◽  
Xu Ma ◽  
...  
Keyword(s):  
In Situ Stabilization ◽  
Novel Method

The initiation of basal disc formation in Dictyostelium discoideum is an early event in culmination

Development ◽  
1996 ◽  
Vol 122 (3) ◽  
pp. 753-760 ◽  
Author(s):  
K. Jermyn ◽  
D. Traynor ◽  
J. Williams
Keyword(s):  
Early Event ◽  
Double Staining ◽  
Forward Movement ◽  
Basal Disc ◽  
Stage Of Development ◽  
Glucuronidase Reporter ◽  
Tip Formation ◽  
Beta Galactosidase ◽  
Disc Formation

We have analysed expression of the ecmA and ecmB genes of Dictyostelium by enzymatic double staining using beta-galactosidase and beta-glucuronidase reporter gene constructs. Cells expressing the ecmA gene first appear as scattered cells at the mound stage of development and we show that this is also true for cells expressing the ecmB gene. During tip formation the ecmA-expressing cells move to the apex of the mound, while the ecmB-expressing cells accumulate in the base. The ecmB-expressing cells constitute part of the basal disc if the culminant is formed in situ but are discarded if a migratory slug is formed. During slug migration they are replaced by a band of ecmB-expressing cells, situated in the front half of the prespore zone and tightly apposed to the substratum. When culmination is triggered these cells rapidly move to the back half of the prestalk zone, possibly acting as a point of attachment to the substratum. Ultimately, they are joined by cells at the back of the slug, the rearguard cells, to form the basal disc. Thus, contrary to previous belief, basal disc formation is initiated very early during culmination and occurs by the forward movement of cells located in the anterior of the prespore zone.

High Shear in Situ Exfoliation of 2D Gallium Oxide Sheets from Centrifugally Derived Thin Films of Liquid Gallium

2021 ◽  
Author(s):  
Kasturi Vimalanathan ◽  
Timotheos Palmer ◽  
Zoe Gardner ◽  
Irene Ling ◽  
Soraya Rahpeima ◽  
...  
Keyword(s):  
Gallium Oxide ◽  
High Mass ◽  
Liquid Gallium ◽  
The Novel ◽  
Continuous Flow Condition ◽  
Novel Method ◽  
Hydrogen Evolution Reactions ◽  
Insulating Properties ◽  
Microfluidics Platform

Herein, we have explored the use of a microfluidics platform for the exfoliation and oxidation of liquid gallium into ultrathin sheets of gallium oxide under continuous flow condition. The novel method developed here takes advantage of the high mass transfer in liquids and has the potential for creating high yielding thin sheets of oxidised gallium with insulating properties as well as acts as an active catalyst in hydrogen evolution reactions. This highlights the potential utility of the sheets as an alternative to the expensive and scarce noble metal based electrocatalysts

Nutrient and hormone levels in Douglas-fir corrosion cavities, megagametophytes, and embryos during embryony

2005 ◽  
Vol 35 (10) ◽  
pp. 2447-2456 ◽  
Author(s):  
John G Carman ◽  
Gordon Reese ◽  
Rodney J Fuller ◽  
Timnit Ghermay ◽  
Roger Timmis
Keyword(s):  
Pseudotsuga Menziesii ◽  
Dry Mass ◽  
Douglas Fir ◽  
Chemical Analyses ◽  
Dynamic Changes ◽  
Hormone Levels ◽  
Corrosion Cavity ◽  
Novel Method ◽  
Indole 3 Acetic Acid

Gymnospermous embryos are nourished by fluids secreted from the megagametophyte. During early embryony, these fluids occupy the newly formed corrosion cavity. We describe a novel method for extracting corrosion cavity fluid and provide chemical analyses based on extractions from approximately 120 000 Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) megagametophytes. Levels of potassium, phosphorus, calcium, zinc, and iron were higher in corrosion cavity fluid than in whole tissue, but levels of sulphur and manganese were lower. Levels of cyclitols, sucrose equivalents, erythrose, and arabinose were many-fold higher in corrosion cavity fluid than in whole tissues. Ala, Ser, Arg, Glx, and NH3 exceeded 80 mmol/kg dry mass in corrosion cavity fluid. These levels were about 100-fold higher than those found in whole tissues. During early embryony, hormone levels in corrosion cavity fluid were higher than levels observed in whole megagametophytes by 120-fold for indole-3-acetic acid, 53-fold for abscisic acid, and 8- to 10-fold for cytokinins. Nutrient and hormone levels tended to be much higher in the corrosion cavity fluid than would have been predicted based on whole-tissue analyses. Dynamic changes in nutrient and hormone levels occurred over time in the corrosion cavity, and these changes may normalize embryony in situ.

scholarly journals Poly(catecholamine) Coating and Biofunctionalization of CsPbBr3 Microcrystals

2021 ◽  
Author(s):  
Sangyeon Cho ◽  
Seok-Hyun Yun
Keyword(s):  
Lipid Bilayers ◽  
Environmental Stability ◽  
Live Cells ◽  
Functional Coatings ◽  
Common Strategy ◽  
Novel Method ◽  
Halide Perovskites ◽  
The Stability ◽  
New Applications

<p>Lead halide perovskites (LHP) microcrystals are promising materials for various optoelectronic applications. Surface coating on particles is a common strategy to improve their functionality and environmental stability, but LHP is not amenable to most coating chemistries because of its intrinsic weakness against polar solvents. Here, we describe a novel method of synthesizing LHP microcrystals in a super-saturated polar solvent using sonochemistry and applying various functional coatings on individual microcrystals <i>in situ</i>. We synthesize cesium lead bromine perovskite (CsPbBr<sub>3</sub>) microparticles capped with organic poly-norepinephrine (pNE) layers. The catechol group of pNE coordinates to bromine-deficient lead atoms, forming a defect-passivating and diffusion-blocking shell. The pNE layer enhances the stability of CsPbBr<sub>3</sub> in water by 2,000-folds, enabling bright luminescence and lasing from single microcrystals in water. Furthermore, the pNE shell permits biofunctionalization with proteins, small molecules, and lipid bilayers. Luminescence from CsPbBr<sub>3</sub> microcrystals is sustained in water over 1 hour and observed in live cells. The functionalization method may enable new applications of LHP particles in water-rich environments.<b></b></p>

A Novel Method for the In situ Generation of Alkoxydialkylboranes and Their Use in the Selective Preparation of 1,3-synDiols

1987 ◽  
Vol 16 (10) ◽  
pp. 1923-1926 ◽  
Author(s):  
Kau-Ming Chen ◽  
Karl G. Gunderson ◽  
Goetz E. Hardtmann ◽  
Kapa Prasad ◽  
Oljan Repic ◽  
...  
Keyword(s):  
Novel Method ◽  
In Situ Generation

Comparison between the conventional method of extraction of essential oil of Laurus nobilis L. and a novel method which uses microwaves applied in situ, without resorting to an oven

2007 ◽  
Vol 1143 (1-2) ◽  
pp. 36-40 ◽  
Author(s):  
Guido Flamini ◽  
Marianna Tebano ◽  
Pier Luigi Cioni ◽  
Lucia Ceccarini ◽  
Andrea Simone Ricci ◽  
...  
Keyword(s):  
Essential Oil ◽  
Conventional Method ◽  
Laurus Nobilis ◽  
Novel Method ◽  
Method Of Extraction

scholarly journals Quantitative analysis of glyphosate, glufosinate and AMPA in irrigation water by in situ derivatization–dispersive liquid–liquid microextraction combined with UPLC-MS/MS

2018 ◽  
Vol 10 (5) ◽  
pp. 554-561 ◽  
Author(s):  
Edgar Pinto ◽  
António Gomes Soares ◽  
Isabel M. P. L. V. O. Ferreira
Keyword(s):  
Irrigation Water ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Aminomethylphosphonic Acid ◽  
Liquid Chromatography Tandem Mass ◽  
Novel Method ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

A novel method was developed for the sensitive, cheap and fast quantitation of glyphosate, glufosinate and aminomethylphosphonic acid (AMPA) in irrigation water by in situ derivatization and dispersive liquid–liquid microextraction (DLLME) combined with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS).

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