Detection of estrogen receptor (ER) in the rat brain using rat anti-ER monoclonal IgG with the unlabeled antibody method

1991 ◽  
Vol 96 (2) ◽  
pp. 157-162 ◽  
Author(s):  
W. W. Henry ◽  
K. L. Medlock ◽  
D. M. Sheehan ◽  
A. C. Scallet
Keyword(s):  
Estrogen Receptor ◽  
Rat Brain ◽  
Antibody Method ◽  
Unlabeled Antibody

Breast cancers negative for estrogen receptor but positive for progesterone receptor, a true entity?

1987 ◽  
Vol 5 (4) ◽  
pp. 662-666 ◽  
Author(s):  
D T Kiang ◽  
R Kollander
Keyword(s):  
Estrogen Receptor ◽  
Progesterone Receptor ◽  
Staining Method ◽  
Binding Assay ◽  
Assay Method ◽  
Breast Cancers ◽  
Antibody Method ◽  
Steroid Binding ◽  
Rare Group ◽  
Er Status

By the conventional steroid-binding assay method for receptor, 3% of 1,095 primary breast cancers (or 10.6% of 263 premenopausal tumors) were classified as negative for estrogen receptor (ER), but positive for progesterone receptor (PR). The true ER status in this rare group of tumors was further investigated by the enzyme-immunoassay (EIA) or immunocytochemical (ICA) staining method using monoclonal antibodies H222 and D547. Immunoreactive ER was present in nine ER-/PR+ tumors studied, whereas it was not detectable in nine age-matched ER-/PR- tumors. Immunoreactive ER was also present in 24 ER+ breast cancers studied, and was particularly higher in tumors that were PR+. Measurement of immunoreactive ER by monoclonal antibody method provides certain advantages over the conventional dextran-coated charcoal (DCC) method, especially in ER-/PR+ tumors.

scholarly journals Estrogen Receptor-β Immunoreactivity in Luteinizing Hormone-Releasing Hormone Neurons of the Rat Brain

Endocrinology ◽  
2001 ◽  
Vol 142 (7) ◽  
pp. 3261-3264 ◽  
Author(s):  
Erik Hrabovszky ◽  
Annamária Steinhauser ◽  
Klaudia Barabás ◽  
Paul J. Shughrue ◽  
Sandra L. Petersen ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Luteinizing Hormone ◽  
Rat Brain ◽  
Estrogen Receptor Β ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone

Localization of lipophosphonoglycan in membranes of Acanthamoeba by using specific antibodies

1981 ◽  
Vol 1 (4) ◽  
pp. 358-369
Author(s):  
C F Bailey ◽  
B Bowers
Keyword(s):  
Protein A ◽  
Contractile Vacuole ◽  
Membrane Perturbation ◽  
Cytoplasmic Vacuoles ◽  
Cytoplasmic Surface ◽  
Antibody Method ◽  
Membrane Compartment ◽  
Vacuolar Membranes ◽  
Indirect Immunofluorescent ◽  
Unlabeled Antibody

Antibodies against two electrophoretically distinct forms of lipophosphonoglycan (LPG) were produced in rabbits. Antibody specificity was demonstrated by the coupled antibody 125I-protein A assay (Adair et al., J. Cell Biol. 79:281-285, 1978). Indirect immunofluorescent labeling of intact Acanthamoeba showed that antibodies to both LPG components had the same uniform distribution on the cell surface. Both antibodies also bound to the cytoplasmic surface of isolated phagosomes. The location of LPG in other membranes of the amoeba was demonstrated on sections by the unlabeled antibody method. Although LPG was absent from the nuclear membrane, virtually all of the internal vacuole membranes were labeled, including the contractile vacuole. Antibodies directed against LPG were utilized to label lipophosphonoglycan in the plasma membrane of living amoebae. Labeled membrane was internalized and then localized by immunofluorescence in cytoplasmic vacuoles within 10 min of incubation. Although these results are evidence for exchange between plasma and cytoplasmic vacuolar membranes, the contractile vacuole remained unlabeled and can be considered, therefore, a separate membrane compartment. Concanavalin A also was bound and internalized by the amoeba, but electron microscopy showed that this label caused pronounced membrane perturbation, limiting its usefulness as a membrane marker in this system.

Estrogen receptor (ER) subtype agonists alter monoamine levels in the female rat brain

2010 ◽  
Vol 122 (5) ◽  
pp. 310-317 ◽  
Author(s):  
Laura S. Lubbers ◽  
Peter T. Zafian ◽  
Claris Gautreaux ◽  
Marisa Gordon ◽  
Stephen E. Alves ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Rat Brain ◽  
Female Rat

scholarly journals Localization of the loosely bound nuclear proteins of rat brain: an immunocytochemical ultrastructural study.

1980 ◽  
Vol 28 (6) ◽  
pp. 552-556 ◽  
Author(s):  
D Cocchia ◽  
F Michetti
Keyword(s):  
Rat Brain ◽  
Active Regions ◽  
Antigenic Determinants ◽  
Brain Nuclei ◽  
Liver Nuclei ◽  
Pap Method ◽  
The One ◽  
Unlabeled Antibody ◽  
The Brain ◽  
Isolated Liver

Antibodies against the loosely bound subnuclear protein fraction (0.35 M NaCl-extractable subnuclear fraction) of rat brain were raised in rabbits, and the ultrastructural distribution of the antigenic determinants in rat cerebellum was studied using the unlabeled antibody peroxidase-antiperoxidase (PAP) method. A localization restricted to the nucleus was observed. Both neuronal and neuroglial nuclei exhibited antigens, whereas nuclei of pericytes and endothelial cells did not. The immunoreaction product was homogeneously distributed in dispersed chromatin and was absent from condensed chromatin, suggesting that the antigens were confined to the active regions of the genoma. The outer nuclear membrane and the nucleolus appeared to be free of the antigens, while a perinucleolar ring of immunoreaction was detectable. Liver preparations showed a nuclear reaction markedly weaker than the one for brain nuclei. Adsorption of the serum with isolated liver nuclei nullified the reactivity in liver tissue, whereas a sharp reaction was still observed in the cerebellum, indicating the subcellular reaction under examination to contain antigens specifically concentrated in the nervous system or unique to the brain.

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