HSP12, a new small heat shock gene of Saccharomyces cerevisiae: Analysis of structure, regulation and function

1990 ◽  
Vol 223 (1) ◽  
pp. 97-106 ◽  
Author(s):  
Uta M. Praekelt ◽  
Peter A. Meacock
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Heat Shock Gene ◽  
Shock Gene ◽  
Structure Regulation ◽  
And Function

Transcriptional regulation of an hsp70 heat shock gene in the yeast Saccharomyces cerevisiae

1987 ◽  
Vol 7 (5) ◽  
pp. 1906-1916
Author(s):  
M R Slater ◽  
E A Craig
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Promoter Region ◽  
Inducible Expression ◽  
Proximal Promoter ◽  
Heat Shock Gene ◽  
Heat Shock Element ◽  
Yeast Saccharomyces Cerevisiae ◽  
Hybrid Promoter ◽  
Shock Gene

The yeast Saccharomyces cerevisiae contains three heat-inducible hsp70 genes. We have characterized the promoter region of the hsp70 heat shock gene YG100, that also displays a basal level of expression. Deletion of the distal region of the promoter resulted in an 80% drop in the basal level of expression without affecting expression after heat shock. Progressive-deletion analysis suggested that sequences necessary for heat-inducible expression are more proximal, within 233 base pairs of the initiation region. The promoter region of YG100 contains multiple elements related to the Drosophila melanogaster heat shock element (HSE; CnnGAAnnT TCnnG). Deletion of a proximal promoter region containing one element, HSE2, eliminated most of the heat-inducible expression of YG100. The upstream activation site (UAS) of the yeast cytochrome c gene (CYC1) can be substituted by a single copy of HSE2 plus its adjoining nucleotides (UASHS). This hybrid promoter displayed a substantial level of expression before heat shock, and the level of expression was elevated eightfold by heat shock. YG100 sequences that flank UASHS inhibited basal expression of UASHS in the hybrid promoter but not its heat-inducible expression. This inhibition of basal UASHS activity suggests that negative regulation is involved in modulating expression of this yeast heat shock gene.

scholarly journals Transcriptional regulation of an hsp70 heat shock gene in the yeast Saccharomyces cerevisiae.

1987 ◽  
Vol 7 (5) ◽  
pp. 1906-1916 ◽  
Author(s):  
M R Slater ◽  
E A Craig
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Promoter Region ◽  
Inducible Expression ◽  
Proximal Promoter ◽  
Heat Shock Gene ◽  
Heat Shock Element ◽  
Yeast Saccharomyces Cerevisiae ◽  
Hybrid Promoter ◽  
Shock Gene

The yeast Saccharomyces cerevisiae contains three heat-inducible hsp70 genes. We have characterized the promoter region of the hsp70 heat shock gene YG100, that also displays a basal level of expression. Deletion of the distal region of the promoter resulted in an 80% drop in the basal level of expression without affecting expression after heat shock. Progressive-deletion analysis suggested that sequences necessary for heat-inducible expression are more proximal, within 233 base pairs of the initiation region. The promoter region of YG100 contains multiple elements related to the Drosophila melanogaster heat shock element (HSE; CnnGAAnnT TCnnG). Deletion of a proximal promoter region containing one element, HSE2, eliminated most of the heat-inducible expression of YG100. The upstream activation site (UAS) of the yeast cytochrome c gene (CYC1) can be substituted by a single copy of HSE2 plus its adjoining nucleotides (UASHS). This hybrid promoter displayed a substantial level of expression before heat shock, and the level of expression was elevated eightfold by heat shock. YG100 sequences that flank UASHS inhibited basal expression of UASHS in the hybrid promoter but not its heat-inducible expression. This inhibition of basal UASHS activity suggests that negative regulation is involved in modulating expression of this yeast heat shock gene.

Differential regulation of the 70K heat shock gene and related genes in Saccharomyces cerevisiae

1984 ◽  
Vol 4 (8) ◽  
pp. 1454-1459
Author(s):  
M S Ellwood ◽  
E A Craig
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Heat Shock Gene ◽  
Control Analysis ◽  
Coding Region ◽  
Protein Coding ◽  
Shock Gene ◽  
A Minor ◽  
Flanking Regions ◽  
Beta Galactosidase

Saccharomyces cerevisiae contains a family of genes related to Hsp70, the major heat shock gene of Drosophila melanogaster. The transcription of three of these genes, which show no conservation of sequences 5' to the protein-coding region, was analyzed. The 5' flanking regions from the three genes were fused to the Escherichia coli beta-galactosidase structural gene and introduced into yeasts on multicopy plasmids, putting the beta-galactosidase production under yeast promoter control. Analysis of beta-galactosidase mRNA and protein production in these transformed strains revealed that transcription from the three promoters is differentially regulated. The number of transcripts from one promoter is vastly increased for a brief period after heat shock, whereas mRNA from another declines. Transcripts from a third gene are slightly enhanced upon heat shock; however, multiple 5' ends of the mRNA are found, and a minor species increases in amount after heat shock. Transcription of these promoters in their native state on the chromosome appears to be modulated in the same manner.

scholarly journals Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae

FEBS Journal ◽  
2008 ◽  
Vol 275 (11) ◽  
pp. 2956-2964 ◽  
Author(s):  
Mette M. Jensen ◽  
Marianne S. Christensen ◽  
Bjarne Bonven ◽  
Torben H. Jensen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Heat Shock Gene ◽  
Shock Gene

scholarly journals A heat shock gene from Saccharomyces cerevisiae encoding a secretory glycoprotein.

1992 ◽  
Vol 89 (9) ◽  
pp. 3671-3675 ◽  
Author(s):  
P. Russo ◽  
N. Kalkkinen ◽  
H. Sareneva ◽  
J. Paakkola ◽  
M. Makarow
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Heat Shock Gene ◽  
Shock Gene

scholarly journals Differential regulation of the 70K heat shock gene and related genes in Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (8) ◽  
pp. 1454-1459 ◽  
Author(s):  
M S Ellwood ◽  
E A Craig
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Heat Shock Gene ◽  
Control Analysis ◽  
Coding Region ◽  
Protein Coding ◽  
Shock Gene ◽  
A Minor ◽  
Flanking Regions ◽  
Beta Galactosidase

Saccharomyces cerevisiae contains a family of genes related to Hsp70, the major heat shock gene of Drosophila melanogaster. The transcription of three of these genes, which show no conservation of sequences 5' to the protein-coding region, was analyzed. The 5' flanking regions from the three genes were fused to the Escherichia coli beta-galactosidase structural gene and introduced into yeasts on multicopy plasmids, putting the beta-galactosidase production under yeast promoter control. Analysis of beta-galactosidase mRNA and protein production in these transformed strains revealed that transcription from the three promoters is differentially regulated. The number of transcripts from one promoter is vastly increased for a brief period after heat shock, whereas mRNA from another declines. Transcripts from a third gene are slightly enhanced upon heat shock; however, multiple 5' ends of the mRNA are found, and a minor species increases in amount after heat shock. Transcription of these promoters in their native state on the chromosome appears to be modulated in the same manner.

scholarly journals A heat shock gene from Saccharomyces cerevisiae encoding a secretory glycoprotein.

1992 ◽  
Vol 89 (18) ◽  
pp. 8857-8857 ◽  
Author(s):  
P. Russo ◽  
N. Kalkkinen ◽  
H. Sareneva ◽  
J. Paakkola ◽  
M. Makarow
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Heat Shock Gene ◽  
Shock Gene
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