Differentiation of mouse leydig cell in anterior chamber of the rat eye

1974 ◽  
Vol 146 (2) ◽  
pp. 219-224
Author(s):  
Jos� Russo ◽  
Juan Carlos Rosas
Keyword(s):  
Anterior Chamber ◽  
Leydig Cell ◽  
Mouse Leydig Cell

Role of protein kinase C in the regulation of steroidogenesis in the mouse Leydig cell

1986 ◽  
Vol 113 (1_Suppl) ◽  
pp. S63-S64
Author(s):  
A. K. MUKHOPADHYAY ◽  
H. G. BOHNET
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Leydig Cell ◽  
Kinase C ◽  
Mouse Leydig Cell

AN IMPROVED IN VITRO BIOASSAY METHOD FOR MEASURING LUTEINIZING HORMONE (LH) ACTIVITY USING MOUSE LEYDIG CELL PREPARATIONS

1974 ◽  
Vol 77 (4) ◽  
pp. 655-671 ◽  
Author(s):  
M.-P. Van Damme ◽  
D. M. Robertson ◽  
E. Diczfalusy
Keyword(s):  
Luteinizing Hormone ◽  
Leydig Cell ◽  
Marked Reduction ◽  
Improved Method ◽  
In Vitro Bioassay ◽  
Bioassay Method ◽  
Adult Mice ◽  
Assay Design ◽  
Mouse Leydig Cell

ABSTRACT An improved in vitro bioassay method for the measurement of LH activity is presented. The method is based on the assay of testosterone produced by "Leydig cell" preparations from mouse testes in the presence of added gonadotrophin. The method is significantly improved in terms of sensitivity, precision and practicability when compared to the previously described bioassay method employing decapsulated testes from adult mice. The sensitivity of the improved method is 15 μIU for HCG and 50 μIU for HMG. The useful range of the method is 15–260 μIU for HCG and 50–900 μIU for HMG. Using a 3 + 3 point assay design with each dose in quadruplicate, a mean index of precision (λ̅) of 0.044 was obtained in 19 assays. Human FSH, TSH, ACTH, LTH, STH, oxytocin, vasopressin and LHRH preparations did not influence the bioassay method at levels likely to be found in biological samples. A good correlation was found between estimates obtained by the "Leydig cell" method and by the method using decapsulated testes when various HCG and HMG preparations were used. With the proposed method at least 30 samples can be assayed each week by 2 persons, with a marked reduction in cost.

Estrogen induces secretary proteins in transformed mouse Leydig cell in vivo, but not in vitro

1984 ◽  
Vol 124 (1) ◽  
pp. 277-282 ◽  
Author(s):  
Bunzo Sato ◽  
Yasuko Nishizawa ◽  
Makoto Nakao ◽  
Keizo Noma ◽  
Susumu Kishimoto ◽  
...  
Keyword(s):  
Leydig Cell ◽  
Mouse Leydig Cell

Estrogen Binding Component of Mouse Leydig Cell Tumor: Anin VitroConversion from Nonreceptor to Receptor-Like Molecule*

Endocrinology ◽  
1981 ◽  
Vol 108 (2) ◽  
pp. 612-619 ◽  
Author(s):  
BUNZO SATO ◽  
YOSHIAKI MAEDA ◽  
KEIZO NOMA ◽  
KEISHI MATSUMOTO ◽  
YUICHI YAMAMURA
Keyword(s):  
Leydig Cell ◽  
Leydig Cell Tumor ◽  
Cell Tumor ◽  
Estrogen Binding ◽  
Mouse Leydig Cell ◽  
Binding Component

Identification of unoccupied but transformed nuclear estrogen receptor in cultured mouse leydig cell

1990 ◽  
Vol 35 (5) ◽  
pp. 561-567 ◽  
Author(s):  
Y. Miyashita ◽  
T. Hirose ◽  
H. Kouhara ◽  
S. Kishimoto ◽  
K. Matsumoto ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Leydig Cell ◽  
Nuclear Estrogen Receptor ◽  
Mouse Leydig Cell

Interleukin-18 levels and mouse Leydig cell apoptosis during lipopolysaccharide-induced acute inflammatory conditions

2020 ◽  
Vol 141 ◽  
pp. 103167
Author(s):  
Taketo Inoue ◽  
Michiko Aoyama-Ishikawa ◽  
Mikiko Uemura ◽  
Hayato Yamashita ◽  
Yuka Koga ◽  
...  
Keyword(s):  
Leydig Cell ◽  
Cell Apoptosis ◽  
Interleukin 18 ◽  
Inflammatory Conditions ◽  
Mouse Leydig Cell

scholarly journals Corticotropin-Releasing Factor (CRF) Agonists Stimulate Testosterone Production in Mouse Leydig Cells through CRF Receptor-1*

Endocrinology ◽  
1998 ◽  
Vol 139 (2) ◽  
pp. 651-658 ◽  
Author(s):  
Nadja Heinrich ◽  
Mike R. Meyer ◽  
Jens Furkert ◽  
Annette Sasse ◽  
Michael Beyermann ◽  
...  
Keyword(s):  
Leydig Cell ◽  
Leydig Cells ◽  
Pituitary Cells ◽  
Camp Production ◽  
Cos Cells ◽  
Testosterone Production ◽  
Primary Mouse ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Mouse Leydig Cell

Abstract The influence of CRF on testosterone production in primary mouse Leydig cell cultures was studied, and the type of CRF receptor (CRF-R) involved in this activity was determined. CRF directly stimulated testosterone production in mouse Leydig cells, but did not influence the maximum human (h)CG-induced testosterone production. The effect was time- and dose-dependent, saturable with an EC50 of 2.84 nm for hCRF, antagonized by the CRF antagonist α-helical CRF9–41, and accompanied by intracellular cAMP elevation. The rank order of potency of the natural CRF agonists, hCRF, ovine CRF, sauvagine, and urotensin, corresponded to that of their activities on CRF-R1 in rat pituitary cells and also to that reported for this receptor, but not for CRF-R2, when transfected into various cell lines. Furthermore, the difference in response of mouse Leydig cells to[ 11-d-Thr,12-d-Phe]- and[ 13-d-His,14-d-Leu]-ovine CRF corresponded to that measured when COS cells expressing CRF-R1 were activated, but was considerably smaller than that observed for activation of COS cells expressing CRF-R2α or -R2β. The messenger RNA encoding the mouse CRF-R1 was detected by RT-PCR in mouse Leydig cell preparations. In contrast to mouse Leydig cells, CRF agonists had no influence on the basal testosterone and cAMP production by rat Leydig cells, nor did the agonists or antagonist change the hCG-stimulated testosterone and cAMP production by these cells. It is concluded that mouse Leydig cells express CRF-R1, mediating elevation of testosterone production by CRF agonists through cAMP. Because potencies of CRF agonists in activating mouse Leydig cells were more than 10-fold lower compared with their potencies in stimulating rat pituitary cells, it is suggested that the coupling of the CRF-R1 to intracellular signaling in Leydig cells is different from that in corticotropic pituitary cells, at least in quantitative terms.

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