A gene for cytochrome c oxidase subunit III (COXIII) in broad bean mitochondrial DNA: structural features and sequence evolution

1990 ◽  
Vol 17 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Jane L. Macfarlane ◽  
Jill A. Wahleithner ◽  
David R. Wolstenholme
Zootaxa ◽  
2022 ◽  
Vol 5091 (4) ◽  
pp. 546-558
Author(s):  
ZHAOYANG CHEN ◽  
FENGXIANG LIU ◽  
DAIQIN LI ◽  
XIN XU

This paper reports four new species of the primitively segmented spider genus Songthela from Chongqing Municipality, China, based on morphological characters of both males and females: S. jinyun sp. nov., S. longbao sp. nov., S. serriformis sp. nov. and S. wangerbao sp. nov. We also provide the GenBank accession codes of mitochondrial DNA barcode gene, cytochrome c oxidase subunit I (COI), for the holotype of four new species for future identification.  


2006 ◽  
Vol 52 (6) ◽  
pp. 551-557 ◽  
Author(s):  
Marjorie A. Liénard ◽  
Jean-Marc X.S. Lassance ◽  
Ivan Paulmier ◽  
Jean-François Picimbon ◽  
Christer Löfstedt

2017 ◽  
pp. 155-176
Author(s):  
Miguel Lozano-Terol ◽  
María Juliana Rodríguez-García ◽  
José Galián

En este estudio se analizan dos fragmentos del gen de la citocromo c oxidasa subunidad I (COX1) del ADN mitocondrial de 61 individuos del género Rhynchophorus colectados en la Región de Murcia a fin de determinar su procedencia. El análisis filogenético del fragmento 1 de las muestras de la Región de Murcia conjuntamente con las secuencias disponibles en GenBank indica que los individuos corresponden a la especie Rhynchophorus ferrugineus.Las secuencias de Murcia se colapsan en un único haplotipo (H8 mediterráneo) que aparece dentro del clado de R. ferrugineus. De los análisis filogeográficos se infiere que el origen de los individuos de Murcia es Egipto. Adicionalmente, se examinó una región contigua del COX1 (fragmento 2) en la que las secuencias se colapsaron en dos haplotipos. In this research two fragments of the cytochrome c oxidase subunit I (COX1) gene of the mitochondrial DNA were analyzed in 61 individuals of the genus Rhynchophorus collected in the Region of Murcia with the aim of determining their origin. Phylogenetic analysis of fragment 1 of the samples collected in the Region of Murcia together with the available sequences in GenBank, indicated that these individuals correspond to the species R. ferrugineus. Sequences from Murcia collapsed into the H8 Mediterranean haplotype, which cluster into the R. ferrugineus clade. Phylogeographic analysis shows that the origin of the individuals collected in the Region of Murcia is Egypt. Additionally, a contiguous fragment of COX1 (fragment 2) was analyzed and the sequences collapsed into two haplotypes.


Biochemistry ◽  
1995 ◽  
Vol 34 (50) ◽  
pp. 16298-16305 ◽  
Author(s):  
Shaolong Wu ◽  
Rafael Moreno-Sanchez ◽  
Hagai Rottenberg

2006 ◽  
Vol 80 (1) ◽  
pp. 7-13 ◽  
Author(s):  
K. Ando ◽  
M. Tsunemori ◽  
H. Akahane ◽  
S. Tesana ◽  
H. Hasegawa ◽  
...  

AbstractThe nucleotide sequences of partial 18S, complete internal transcribed spacer region 1 (ITS1), complete 5.8S, complete ITS2 and partial 28S of ribosomal DNA (rDNA) and cytochrome c oxidase subunit 1 of mitochondrial DNA (MCOI) from five species of gnathostomes (G. spinigerum, G. doloresi, G. nipponicum, G. hispidum and G. binucleatum with the former four species being distributed in Japan and Asia) that cause human gnathostomiasis were compared by direct polymerase chain reaction cycle-sequencing. The nucleotide sequences of each region of the18S (613 bp), 5.8S (158 bp) and 28S (598 bp) rDNA from the five species were almost identical. The ITS1 region was different in length for the five species. The nucleotide sequences of each region of ITS2 and partial MCO1 regions were different among the five species. Therefore, these two regions can be used as genetic markers for identification of worms.


1996 ◽  
Vol 271 (2) ◽  
pp. L320-L325 ◽  
Author(s):  
Y. Akiyama ◽  
R. E. Garcia ◽  
A. R. Bazzy

We have previously shown that respiratory training with inspiratory flow-resistive (IFR) loads improves diaphragm performance and is associated with an increase in cytochrome-c oxidase (COX) activity (1). The present study was conducted to define the level at which the increase in COX activity is controlled. Six sheep were trained with IFR loads for 3 h/day for 3 wk. The diaphragm was sampled from the six trained sheep and from six control sheep. Quantitative DNA and RNA slot-blot analyses with mitochondrially coded COX subunit III and nuclearly coded subunit IV probes and immunoblotting with anti-COX holoenzyme antibodies were performed. We found that in the diaphragm the amount of COX subunit proteins coded in either genetic system was greater in the trained than in the control sheep. Neither the amount of mitochondrial DNA nor mRNA for COX subunits was different between the two groups. We conclude that the increase in COX activity in the diaphragm after chronic respiratory training is determined by the amount of subunit proteins, possibly involving translation/degradation of these proteins.


2019 ◽  
Vol 967 ◽  
pp. 59-64
Author(s):  
Yash Munnalal Gupta ◽  
Kittisak Buddhachat ◽  
Surin Peyachoknagul ◽  
Somjit Homchan

The potential of mitochondrial DNA (mtDNA) genes are well-known for species identification and to establish a phylogenetic relationship. The De-novo transcriptome assembly of Acheta domesticus commonly known as house cricket, is provides important segments of DNA fragments from mitochondrial DNA due to higher abundance of its mRNA. When the reference sequence with gene annotation is absent for assembling and aligning desire gene sequences, like in the present case, the most similar sequence is obtained from online insect mitochondrial genome database to find mitochondrial DNA conserved domains of interested gene from high throughput RNA sequencing (RNA-seq) data. The RNA-seq data of Acheta domesticus transcriptome is used to retrieve single nucleotide fragment out of 50,046 assembled contigs to discover three important genes from mtDNA of the house cricket. Present study provides effective workflow to identify genes like cytochrome c oxidase subunit II (COX2), NADH dehydrogenase subunit 2 (ND2), cytochrome c oxidase subunit I (COX1) from mtDNA in large sequence archive of RNA-seq data. These three novel barcode sequences will be useful for genetic identification and evolution investigation of Acheta domesticus. The partial mtDNA sequence with these genes will be important for mitochondrial genome construction.


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