A heat stable serum inhibitor of an antigen antibody reaction of subacute sclerosing panencephalitis

1977 ◽  
Vol 216 (1) ◽  
pp. 51-56 ◽  
Author(s):  
D. Karcher ◽  
M. Noppe ◽  
A. Lowenthal
Keyword(s):  
Subacute Sclerosing Panencephalitis ◽  
Heat Stable ◽  
Serum Inhibitor ◽  
Antibody Reaction ◽  
Antigen Antibody

scholarly journals BIOCHEMICAL STUDY OF CELLULAR ANTIGEN-ANTIBODY REACTION IN TISSUE CULTURE

1960 ◽  
Vol 112 (2) ◽  
pp. 249-255 ◽  
Author(s):  
Akira Tokuda ◽  
Hideo Hayashi ◽  
Kinishiro Matsuba
Keyword(s):  
Tissue Culture ◽  
Protease Activity ◽  
Biochemical Study ◽  
Culture Fluid ◽  
Partial Purification ◽  
Trichloracetic Acid ◽  
Heat Stable ◽  
Antibody Reaction ◽  
Antigen Antibody ◽  
Cellular Antigen

Decrease in the protease activity of the culture fluid observed at later stages of the antigen-antibody reaction is believed to be due to the release of an inhibitor by the cells. The inhibitor was submitted to partial purification: it is heat-stable, non-precipitated by trichloracetic acid and non-dialyzable. It inhibits certain cellular and tissue proteases and papain but is inactive against trypsin. It is suggested that the balance between protease and anti-protease released may determine the intensity, extent, and duration of certain sensitization phenomena.

scholarly journals Evaluation of Shear Horizontal Surface Acoustic Wave Biosensors Using “Layer Parameter” Obtained from Sensor Responses during Immunoreaction

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4924
Author(s):  
Koji Kano ◽  
Hiromi Yatsuda ◽  
Jun Kondoh
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Horizontal Surface ◽  
Propagation Characteristics ◽  
Viscosity Change ◽  
Layer Parameter ◽  
Antibody Reaction ◽  
Antigen Antibody ◽  
Shear Horizontal ◽  
Molecular Dimensions

Shear horizontal surface acoustic wave (SH-SAW) biosensors measure the reaction of capture antibodies immobilized on the sensing surface to capture test molecules (antigens) by using the change in SH-SAW propagation characteristics. SH-SAW displacement exists not only on the SH-SAW propagating surface, but also partially penetrates the specimen liquid to a certain depth, which is determined by the liquid properties of the specimen and the operating frequency of the SH-SAW. This phenomenon is called viscosity penetration. In previous studies, the effect of viscosity penetration was not considered in the measurement of SH-SAW biosensors, and the mass or viscosity change caused by the specific binding of capture antibodies to the target antigen was mainly used for the measurement. However, by considering the effect of viscosity penetration, it was found that the antigen–antibody reaction could be measured and the detection characteristics of the biosensor could be improved. Therefore, this study aims to evaluate the detection properties of SH-SAW biosensors in the surface height direction by investigating the relationship between molecular dimensions and SH-SAW propagation characteristics, which are pseudo-changed by varying the diameter of gold nanoparticles. For the evaluation, we introduced a layer parameter defined by the ratio of the SH-SAW amplitude change to the SH-SAW velocity change caused by the antigen–antibody reaction. We found a correlation between the layer parameter and pseudo-varied molecular dimensions. The results suggest that SH-SAW does not only measure the mass and viscosity but can also measure the size of the molecule to be detected. This shows that SH-SAW biosensors can be used for advanced functionality.

scholarly journals Investigation of the Mechanism for Rapid Antigen-Antibody Reaction Using Electric Field Mixing (EFM)

2019 ◽  
Vol 85 (2) ◽  
pp. 208-212
Author(s):  
Ryuta NAKAMURA ◽  
Takayuki KUSUMI ◽  
Yoshinobu OKUBO ◽  
Hiroshi NANJO ◽  
Yoshihiro MINAMIYA ◽  
...  
Keyword(s):  
Electric Field ◽  
Antibody Reaction ◽  
Antigen Antibody

The study of the antigen-antibody reaction by fluorescence method in LB films for immunosensor

1997 ◽  
Vol 307 (1-2) ◽  
pp. 294-297 ◽  
Author(s):  
G.K. Chudinova ◽  
A.V. Chudinov ◽  
V.V. Savransky ◽  
A.M. Prokhorov
Keyword(s):  
Fluorescence Method ◽  
Lb Films ◽  
Antibody Reaction ◽  
Antigen Antibody
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