Nucleotide sequence of the COX1 gene in Kluyveromyces lactis mitochondrial DNA: evidence for recent horizontal transfer of a group II intron

1991 ◽  
Vol 20 (1-2) ◽  
pp. 99-114 ◽  
Author(s):  
C. M. Hardy ◽  
G. D. Clark-Walker
Keyword(s):  
Mitochondrial Dna ◽  
Nucleotide Sequence ◽  
Horizontal Transfer ◽  
Kluyveromyces Lactis ◽  
Group Ii Intron ◽  
Cox1 Gene ◽  
Dna Evidence ◽  
Group Ii

scholarly journals Group II intron domain 5 facilitates a trans-splicing reaction.

1988 ◽  
Vol 8 (6) ◽  
pp. 2361-2366 ◽  
Author(s):  
K A Jarrell ◽  
R C Dietrich ◽  
P S Perlman
Keyword(s):  
Mitochondrial Dna ◽  
Group Ii Intron ◽  
Intron Sequence ◽  
Trans Splicing ◽  
Group Ii ◽  
Self Splicing ◽  
Yeast Mitochondrial Dna ◽  
Domain 4

A self-splicing group II intron of yeast mitochondrial DNA (aI5g) was divided within intron domain 4 to yield two RNAs that trans-spliced in vitro with associated trans-branching of excised intron fragments. Reformation of the domain 4 secondary structure was not necessary for the trans reaction, since domain 4 sequences were shown to be dispensable. Instead, the trans reaction depended on a previously unpredicted interaction between intron domain 5, the most highly conserved region of group II introns, and another region of the RNA. Domain 5 was shown to be essential for cleavage at the 5' splice site. It stimulated that cleavage when supplied as a trans-acting RNA containing only 42 nucleotides of intron sequence. The relevance of our findings to in vivo trans-splicing mechanisms is discussed.

scholarly journals A group II intron in theNeurosporamitochondrialcolgene: nucleotide sequence and implications for splicing and molecular evolution

1989 ◽  
Vol 17 (22) ◽  
pp. 9087-9099 ◽  
Author(s):  
Deborah J. Field ◽  
Adele Sommerfield ◽  
Barry J. Saville ◽  
Richard A. Collins
Keyword(s):  
Molecular Evolution ◽  
Nucleotide Sequence ◽  
Group Ii Intron ◽  
Group Ii

Group II intron domain 5 facilitates a trans-splicing reaction

1988 ◽  
Vol 8 (6) ◽  
pp. 2361-2366
Author(s):  
K A Jarrell ◽  
R C Dietrich ◽  
P S Perlman
Keyword(s):  
Mitochondrial Dna ◽  
Group Ii Intron ◽  
Intron Sequence ◽  
Trans Splicing ◽  
Group Ii ◽  
Self Splicing ◽  
Yeast Mitochondrial Dna ◽  
Domain 4

A self-splicing group II intron of yeast mitochondrial DNA (aI5g) was divided within intron domain 4 to yield two RNAs that trans-spliced in vitro with associated trans-branching of excised intron fragments. Reformation of the domain 4 secondary structure was not necessary for the trans reaction, since domain 4 sequences were shown to be dispensable. Instead, the trans reaction depended on a previously unpredicted interaction between intron domain 5, the most highly conserved region of group II introns, and another region of the RNA. Domain 5 was shown to be essential for cleavage at the 5' splice site. It stimulated that cleavage when supplied as a trans-acting RNA containing only 42 nucleotides of intron sequence. The relevance of our findings to in vivo trans-splicing mechanisms is discussed.

scholarly journals Mutation of the conserved first nucleotide of a group II intron from yeast mitochondrial DNA reduces the rate but allows accurate splicing

1993 ◽  
Vol 268 (16) ◽  
pp. 11929-11938
Author(s):  
C.L. Peebles ◽  
S.M. Belcher ◽  
M. Zhang ◽  
R.C. Dietrich ◽  
P.S. Perlman
Keyword(s):  
Mitochondrial Dna ◽  
Group Ii Intron ◽  
Group Ii ◽  
Yeast Mitochondrial Dna
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