Platelet 3H-imipramine binding and steroid hormones serum concentrations during the menstrual cycle

1986 ◽  
Vol 88 (1) ◽  
pp. 86-89 ◽  
Author(s):  
Marie-France Poirier ◽  
Chawki Benkelfat ◽  
Anne-Marie Galzin ◽  
Salomon Z. Langer
Keyword(s):  
Menstrual Cycle ◽  
Steroid Hormones ◽  
Serum Concentrations ◽  
Imipramine Binding

CHANGES IN THE CONCENTRATION OF PITUITARY AND STEROID HORMONES IN THE FOLLICULAR FLUID OF HUMAN GRAAFIAN FOLLICLES THROUGHOUT THE MENSTRUAL CYCLE

1975 ◽  
Vol 64 (3) ◽  
pp. 555-571 ◽  
Author(s):  
K. P. McNATTY ◽  
W. M. HUNTER ◽  
A. S. McNEILLY ◽  
R. S. SAWERS
Keyword(s):  
Menstrual Cycle ◽  
Steroid Hormones ◽  
Follicular Fluid ◽  
Follicle Stimulating Hormone ◽  
Secretory Activity ◽  
Follicular Phase ◽  
Hormonal Changes ◽  
Peripheral Plasma ◽  
Before And After ◽  
Late Follicular Phase

SUMMARY The concentrations of FSH, LH, prolactin, oestradiol and progesterone were measured in peripheral plasma and follicular fluid of women throughout the menstrual cycle. With the exception of prolactin, concentrations of pituitary and steroid hormones in follicular fluid correlated with those in peripheral plasma. Follicle-stimulating hormone was present in a greater number of small follicles ( < 8 mm) during or just after the peaks of FSH in peripheral plasma. During the mid-follicular phase the concentration of both FSH and oestradiol in fluid from large follicles ( ≥ 8 mm) was high. During the late follicular phase the large follicles ( ≥ 8 mm) contained high amounts of progesterone in addition to oestradiol, low physiological levels of prolactin, and concentrations of LH and FSH about 30 and 60% respectively of those found in plasma. By contrast no large 'active' follicles ( ≥ 8 mm) were found during the luteal phase although many contained both LH and FSH. Luteinizing hormone was present in a proportion of small follicles ( < 8 mm) during the late follicular and early luteal but not at other stages of the menstrual cycle. It is suggested that a precise sequence of hormonal changes occur within the microenvironment of the developing Graafian follicle; the order in which they occur may be of considerable importance for the growth of that follicle and secretory activity of the granulosa cells both before and after ovulation.

Simultaneous determination of five sex hormones in human serum by radioimmunoassay after chromatography on Lipidex-5000.

1976 ◽  
Vol 22 (1) ◽  
pp. 32-38 ◽  
Author(s):  
D Apter ◽  
O Jánne ◽  
P Karvonen ◽  
R Vihko
Keyword(s):  
Menstrual Cycle ◽  
Human Serum ◽  
Simultaneous Determination ◽  
Sex Hormones ◽  
Oral Contraceptives ◽  
Light Petroleum ◽  
Serum Concentrations ◽  
Coefficients Of Variation ◽  
Normal Men

Abstract We describe a method for determination of pregnenolone, progesterone, 17alpha-hydroxyprogesterone, testosterone, and 5alpha-dihydrotestosterone in 1-2 ml of serum from male or female. Using microcolumns of Lipidex-5000 (hydroxyalkoxypropyl Sephadex, 0.5 g) and light petroleum/chloroform (97/3) as the solvent during chromatography, we resolved these five steroids into four fractions, with pregnenolone and 5alpha-dihydrotestosterone eluting together. By use of selected antibodies, the latter two steroids were also determined specifically. Use of microcolumns allowed minimization of solvent volumes and sample transfers. Consequently, blank values for all the five steroids were negligible. Lowest measureable concentrations (in ng/liter) were: pregnenolone 100, progesterone 25, 17alpha-hydroxyprogesterone 50, testosterone 25, and 5alpha-dihydrotestosterone 25. Intra-assay and inter-assay coefficients of variation ranged from 5 to 9% and 10 to 15%, respectively, for the five steroids. Serum concentrations of these steroids are given for women in the follicular and luteal phases of the menstrual cycle and for women on oral contraceptives of the combination type, as well as for normal men.

scholarly journals Concentrations of steroid hormones, and of prolactin, in washings of the human uterus during the menstrual cycle

Reproduction ◽  
1986 ◽  
Vol 78 (1) ◽  
pp. 21-25 ◽  
Author(s):  
B. A. Stone ◽  
O. M. Petrucco ◽  
R. F. Seamark ◽  
B. M. Godfrey
Keyword(s):  
Menstrual Cycle ◽  
Steroid Hormones ◽  
Human Uterus

Lamotrigine Serum Concentrations Throughout the Menstrual Cycle-A Study of 2 Cases

2006 ◽  
Vol 29 (3) ◽  
pp. 160-162 ◽  
Author(s):  
Arne Reimers ◽  
Eylert Brodtkorb ◽  
Grethe Helde ◽  
Olav Spigset
Keyword(s):  
Menstrual Cycle ◽  
Serum Concentrations

scholarly journals Steroid Diagnostics of 21st Century in the Light of Their New Roles and Analytical Tools

2020 ◽  
pp. S193-S203 ◽  
Author(s):  
M. DUŠKOVÁ ◽  
L. KOLÁTOROVÁ ◽  
M. ŠIMKOVÁ ◽  
M. ŠRÁMKOVÁ ◽  
M. MALÍKOVÁ ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Steroid Hormones ◽  
21St Century ◽  
Reference Values ◽  
Current Technology ◽  
Amount Of Information ◽  
Analytical Phase ◽  
Analytical Tools ◽  
Fertile Women

The determination of steroid hormones and subsequent interpretation of results is accompanied by a range of difficulties. The amount of information that current technology can provide on the circulating concentrations of more than a hundred various steroid compounds can lead to problems with interpretation. The aim of this study is to help provide orientation in this maze of data on steroid hormones. First we focus on specific aspects arising from the pre-analytical phase of steroid determination that need to be considered when planning sampling, whether for diagnostics or research. Then, we provide a brief summary of the characteristics and diagnostic relevance of several steroid hormones and/or their metabolites: pregnenolone, 17α-hydroxy-pregnenolone, dehydroepiandrosterone, hydroxyderivatives of dehydroepiandrosterone, androstenedione, testosterone, estrone, estradiol, estriol, cortisol, cortisone, which in our institute are determined with validated LC-MS/MS methods. For these steroids, we also provide newly calculated reference values in fertile women according to the phase of their menstrual cycle.

scholarly journals Serum inhibin B in polycystic ovary syndrome as a potential marker of ovarian dysfunction

2008 ◽  
Vol 136 (7-8) ◽  
pp. 379-383
Author(s):  
Aleksandar Cetkovic
Keyword(s):  
Menstrual Cycle ◽  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Inhibin B ◽  
Control Group ◽  
Serum Concentrations ◽  
Ovarian Dysfunction ◽  
Ovary Syndrome ◽  
The Third ◽  
Healthy Women

INTRODUCTION Polycystic ovary syndrome (PCOS) is one of the most common causes of anovulation, infertility and hyperandrogenism, and the prevalence of this condition in women of reproductive is 5-10%. The growth of early ovarian antral follicles is arrested and dominant follicle selection is disturbed in this syndrome. OBJECTIVE The aim of this study is to investigate whether inhibin B serum concentrations represent the extent of ovarian abnormalities in patients with PCOS. METHOD Inhibin B serum concentrations on the third day of spontaneous menstrual cycle and other endocrine characteristics were compared between 20 patients with PCOS and 19 healthy women in the control group. RESULTS Inhibin B concentrations were not significantly different between women with PCOS and women in the control group. In patients with PCOS there was statistically significant correlation between serum inhibin B and LH (r=0.514; p=0.021). There were no positive correlations between inhibin B and others endocrine parameters in patients with PCOS (FSH, E2, T, androstenedione). CONCLUSION Inhibin B serum concentrations on the third day of spontaneous menstrual cycle in women with PCOS are not different from the concentrations in healthy women. Serum Inhibin B levels in patients with PCOS are only slightly correlated with the endocrine markers of the disease so it could not represent the magnitude of ovarian dysfunction in this syndrome.

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