Studies on reaction and binding of monoamines after fixation and processing for electron microscopy with special reference to fixation with potassium permanganate

Histochemie ◽  
1968 ◽  
Vol 16 (1) ◽  
pp. 45-67 ◽  
Author(s):  
T. Hökfelt ◽  
G. Jonsson
Keyword(s):  
Electron Microscopy ◽  
Special Reference ◽  
Potassium Permanganate

A Study of Potassium Permanganate ‘Fixation’ for Electron Microscopy

1960 ◽  
Vol s3-101 (55) ◽  
pp. 241-250
Author(s):  
S. BRADBURY ◽  
G. A. MEEK
Keyword(s):  
Electron Microscopy ◽  
Granular Material ◽  
Potassium Permanganate ◽  
Electron Micrographs ◽  
Protein Complexes

The action of buffered potassium permanganate as a fixative for electron microscopy has been investigated. Electron contrast has been shown to be produced by the deposition of granular material in the tissue. The particles are about 50 Å in diameter. Actual fixation of the tissue is performed by the dehydrating alcohol. Histochemical studies have shown that RNA and histones are removed, whereas phospholipid-protein complexes are ‘unmasked’. The reaction of the permanganate with unmasked protein gives rise to high membrane contrast in electron micrographs.

Applications of scanning electron microscopy in taxonomy with special reference to family Euphorbiaceae

2020 ◽  
Vol 83 (9) ◽  
pp. 1066-1078 ◽  
Author(s):  
Tahira Alyas ◽  
Shabnum Shaheen ◽  
Uswah Amber ◽  
Nidaa Harun ◽  
Sana Khalid ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Special Reference ◽  
Scanning Electron

Variation in pollen wall ultrastructure in New World Senecioneae (Asteraceae), with special reference to Packera

1997 ◽  
Vol 75 (5) ◽  
pp. 730-735 ◽  
Author(s):  
J. F. Bain ◽  
B. S. Tyson ◽  
D. F. Bray
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Chromosome Number ◽  
Special Reference ◽  
New World ◽  
Pollen Wall ◽  
Wall Structure ◽  
Transmission Electron ◽  
Wall Ultrastructure ◽  
Base Chromosome Number

The structure of the pollen wall as revealed by transmission electron microscopy is presented for 34 species representing two subtribes and 12 genera of New World Senecioneae. The genus Packera (=aureoid Senecio), with the exception of Packera zimapanica, is characterized by the helianthoid wall structure. In light of these results, the disposition of the latter species requires review. The genera Robinsonecio and Telanthophora of the subtribe Tussilagininae also possess helianthoid pollen. All other taxa surveyed have senecioid pollen. So far as known no taxa exist within the tribe Senecioneae with a base chromosome number of n = 20 and helianthoid pollen. This suggests that the evolution of Packera may have involved hybridization between members of the two subtribes Senecionineae and Tussilagininae. Key words: Asteraceae, Packera, Senecioneae, pollen, TEM, systematics.

Elastomer Blends

2002 ◽  
Vol 75 (3) ◽  
pp. 365-427 ◽  
Author(s):  
Duryodhan Mangaraj
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Electrical Conductivity ◽  
Special Reference ◽  
Transport Properties ◽  
Commercial Application ◽  
Solution Properties ◽  
Polymer Miscibility ◽  
Mechanical Methods

Abstract Blending of elastomers is often used to enhance he performance characteristics of rubber products. This article reviews the thermodynamic principles underlying polymer-polymer miscibility, with special reference to elastomer blends. It discusses the distribution of fillers, additives and curatives in different phases of the blend and its effect on the properties of the vulcanizate. It describes the different methods used in preparing elastomer blends, with special emphasis on compatibilization. Characterization of blends using optical and electron microscopy, solution properties, thermal and dynamic mechanical methods have been described along with results. The effect of blending on mechanical properties, hysteresis, adhesion and tack, transport properties and electrical conductivity has been discussed. Past attempts for property enhancement by blending elastomers have been reviewed, followed by a discussion of commercial application of elastomer blends.

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