Electron cytochemistry of calcium uptake in the fragmented sarcoplasmic reticulum

Histochemie ◽  
1971 ◽  
Vol 28 (1) ◽  
pp. 55-67 ◽  
Author(s):  
B. Agostini ◽  
W. Hasselbach
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Uptake ◽  
Electron Cytochemistry

Kinetics of calcium uptake by isolated sarcoplasmic reticulum vesicles using flash photolysis of caged ATP

Biochemistry ◽  
1983 ◽  
Vol 22 (23) ◽  
pp. 5254-5261 ◽  
Author(s):  
Dorothy H. Pierce ◽  
Antonio Scarpa ◽  
Michael R. Topp ◽  
J. Kent Blasie
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Uptake ◽  
Flash Photolysis ◽  
Caged Atp ◽  
Kinetics Of

scholarly journals Calcium Uptake Activity of Canine Myocardial Sarcoplasmic Reticulum in the Presence of Anesthetic Agents

1968 ◽  
Vol 23 (5) ◽  
pp. 597-604 ◽  
Author(s):  
RICHARD F. LAIN ◽  
MICHAEL L. HESS ◽  
EDWARD W. GERTZ ◽  
F. NORMAN BRIGGS
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Uptake ◽  
Anesthetic Agents ◽  
Uptake Activity

Functions of selected biochemical systems from the exercised-trained dog heart

1977 ◽  
Vol 42 (3) ◽  
pp. 426-431 ◽  
Author(s):  
L. A. Sordahl ◽  
G. K. Asimakis ◽  
R. T. Dowell ◽  
H. L. Stone
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Transport ◽  
Calcium Uptake ◽  
Atp Synthesis ◽  
Myocardial Cell ◽  
Ruthenium Red ◽  
Mitochondrial Membranes ◽  
Sedentary Control ◽  
Isolated Mitochondria ◽  
Biochemical Systems

Mitochondria and sarcoplasmic reticulum (SR) fractions were isolated from exercised-trained (E-T) and sedentary control dog hearts. Measurements of mitochondrial respiratory functions indicated no changes in energy-producing (ATP synthesis) capacity in mitochondria from E-T compared to control dog hearts. However, the ability of isolated mitochondria from E-T hearts to retain accumulated calcium was markedly decreased compared to controls. Inhibition of mitochondrial rates of calcium uptake with the inhibitor, ruthenium red, revealed fewer binding and/or transport sites in mitochondrial membranes from exercised-trained heart preparations. ATP-dependent binding (- oxalate) and uptake (+ oxalate) of calcium by SR preparations from E-T hearts were unchanged compared to controls. In contrast, significant differences in the rates of release of bound calcium were found in SR isolated from E-T hearts. Total myocardial protein, nucleic acids, and connective tissue levels were unchanged in E-T hearts compared to controls. The results suggest subtle changes are occurring in the energy-utilizing mechanism(s) involving calcium transport of the myocardial cell during exercise training. These changes may be related to alterations in the performance of the exercised-trained heart.

The Effect of Calcium and Phosphate on the Biphasic Calcium Uptake by the Sarcoplasmic Reticulum

1975 ◽  
Vol 30 (11-12) ◽  
pp. 777-780 ◽  
Author(s):  
Pierre Ermier ◽  
Wilhelm Hasselbach
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Inorganic Phosphate ◽  
Calcium Uptake ◽  
Initial Rate ◽  
Maximum Amplitude ◽  
Phosphate Concentration ◽  
Free Calcium ◽  
Physiological Range ◽  
External Calcium

Abstract The amplitude of the fast uptake and the initial rate of the slow uptake increase with in­ creasing free calcium concentrations, up to 30 μᴍ. In that range, both processes are correlated to each other. At higher concentrations, the slow uptake is more inhibited than the fast uptake. The fast uptake shows a maximum amplitude which remains unchanged in the presence of phosphate. The slow uptake leads to a nearly complete depletion of the external calcium, and its rate is proportional to the phosphate concentration, even at physiological range. The sarcoplasmic ATPase liberates inorganic phosphate and the slow uptake

Sign in / Sign up

Export Citation Format

Share Document