Ruthenium red staining of rat glomerulus

1970 ◽  
Vol 22 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Bruce A. Fowler
Keyword(s):  
Ruthenium Red ◽  
Ruthenium Red Staining

Ruthenium Red Staining of Human Hard Keratin Fibers

1982 ◽  
Vol 40 ◽  
pp. 278-279
Author(s):  
M. C. Buhrer ◽  
R. A. Mathews
Keyword(s):  
Human Hair ◽  
Ruthenium Red ◽  
Acid Mucopolysaccharides ◽  
Biochemical Studies ◽  
Keratin Fibers ◽  
Ruthenium Red Staining ◽  
Extracellular Materials

Ruthenium red has been used as a stain to demonstrate a variety of extracellular materials, especially acid mucopolysaccharides. It also reacts with certain intracellular and extracellular lipids. Since biochemical studies in our laboratory demonstrated the presence of a variety of monosaccharides in human hair ruthenium red staining procedures were adopted in order to evaluate the presence and morphological location of acid oligosaccharides in the keratinized aspect of hair.

Structure, properties and enhanced expression of galactose-binding C-type lectins in mucous cells of gills from freshwater Japanese eels (Anguilla japonica)

2001 ◽  
Vol 360 (1) ◽  
pp. 107-115 ◽  
Author(s):  
Abinash Chandra MISTRY ◽  
Shinji HONDA ◽  
Shigehisa HIROSE
Keyword(s):  
Ruthenium Red ◽  
Anguilla Japonica ◽  
Amino Acid Residues ◽  
Mucous Cells ◽  
Cdna Subtraction ◽  
Competitive Binding Assay ◽  
Enhanced Expression ◽  
Ruthenium Red Staining ◽  
High Level ◽  
Cdna Subtraction Library

Using a Japanese-eel (Anguilla japonica) gill cDNA subtraction library, two novel β-d-galactose-binding lectins were identified that belong to group VII of the animal C-type lectin family. The eel C-type lectins, termed eCL-1 and eCL-2, are simple lectins composed of 163 amino acid residues, including a 22-residue signal peptide for secretion and a single carbohydrate-recognition domain (CRD) of ∼ 130 residues typical of C-type lectins. The galactose specificity of the CRD was suggested by the presence of a QPD motif and confirmed by a competitive binding assay. Using Ruthenium Red staining, the lectins were shown to bind Ca2+ ions. SDS/PAGE showed that native eCL-1 and eCL-2have an SDS-resistant octameric structure (a tetramer of disulphide-linked dimers). Northern and Western blot analyses demonstrated high-level expression of eCL-1 and eCL-2 mRNAs and their protein products in gills from freshwater eels, which decreased markedly when the eels were transferred from freshwater to seawater. Immunohistochemistry showed that the eel lectins are localized in the exocrine mucous cells of the gill.

Morphology and ultrastructure of oral strains of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus

1980 ◽  
Vol 30 (2) ◽  
pp. 588-600
Author(s):  
S C Holt ◽  
A C Tanner ◽  
S S Socransky
Keyword(s):  
Electron Microscopy ◽  
Ruthenium Red ◽  
Actinobacillus Actinomycetemcomitans ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Large Numbers ◽  
Haemophilus Aphrophilus ◽  
Ruthenium Red Staining ◽  
Amorphous Surface ◽  
Scanning Electron

Selected human oral and nonoral strains of the genera Actinobacillus and Haemophilus were examined by transmission and scanning electron microscopy. The strains examined were morphologically identical to recognized Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, and Haemophilus paraphrophilus. By transmission electron microscopy, the cells were typically gram negative in morphology, with several strains possessing some extracellular ruthenium red-staining polymeric material. Numerous vesicular structures, morphologically identical to lipopolysaccharide vesicles, were seen to originate from and be continuous with the surface of the outer membrane. Large numbers of these vesicles were also found in the external environment. Scanning electron microscopic observations revealed that both actinobacilli and haemophili possessed surface projections and an amorphous surface material which connected and covered adjacent cells.

Observation of exopolysaccharides (EPS) from Lactobacillus helveticus SBT2171 using the Tokuyasu method

Microscopy ◽  
2020 ◽  
Vol 69 (5) ◽  
pp. 286-290
Author(s):  
Takamichi Kamigaki ◽  
Akihiro Ogawa
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Colloidal Gold ◽  
Ruthenium Red ◽  
Lactobacillus Helveticus ◽  
Ruthenium Red Staining ◽  
Observation Method ◽  
Β Lactoglobulin

Abstract Some species of lactic acid bacteria used for the production of natural cheese produce exopolysaccharides (EPS). Electron microscopy is useful for analyzing the microstructure of EPS produced by lactic acid bacteria. However, pretreatments used to observe the microstructure of EPS by electron microscopy, such as dehydration and resin embedding, can result in EPS flowing out easily from the cell. Therefore, in this study, the Tokuyasu method was conducted on cryosection to reduce EPS outflow. Two types of observation method, namely, using lectin and ruthenium red, were conducted in an attempt to observe EPS produced by Lactobacillus helveticus SBT2171. Observation using the lectin method confirmed that colloidal gold particles conjugated with a lectin recognizing β-galactoside were present in the capsule. Structures that appeared to be β-galactoside-containing slime polysaccharides that were released from the cell wall were also observed. Observation using ruthenium red showed that capsular polysaccharides (CPS) in the capsule were present as a net-like structure. Colloidal gold conjugation with an anti-β-lactoglobulin antibody, in addition to ruthenium red staining, allowed the identification of slime polysaccharides released from the cell wall in the milk protein network derived from the culture medium. Based on these results, the Tokuyasu method was considered to be a useful pretreatment method to clarify and observe the presence of EPS. In particular, both CPS in the capsule and slime exopolysaccharides released from the cell wall were visualized.

scholarly journals An application of modified ruthenium red staining for mouse calvaria.

1984 ◽  
Vol 26 (3) ◽  
pp. 941-946
Author(s):  
Tsuneo Takahashi ◽  
Seigyo So ◽  
Kazuto Takahashi ◽  
Masayoshi Kumegawa
Keyword(s):  
Ruthenium Red ◽  
Mouse Calvaria ◽  
Ruthenium Red Staining

THE BEHAVIOUR OF RABBIT PLASMINOGEN AT THE LUMINAL SURFACE OF RABBIT AORTA IN VIVO BEFORE AND AFTER BALLOON-CATHETER INJURY

1987 ◽  
Author(s):  
Mark W C Hatton ◽  
Susan Moar ◽  
Mary Richardson
Keyword(s):  
White Male ◽  
Balloon Catheter ◽  
Rabbit Aorta ◽  
Ruthenium Red ◽  
Transmission Electron ◽  
Before And After ◽  
Ruthenium Red Staining ◽  
Balloon Catheter Injury

A previous study from this laboratory has identified the susceptibility of the de-endothelialised aorta, particularly the proteoglycan (PG) components of the extracellular matrix (ECM), to proteolytic damage if exposed to plasmin in vitro. To explore the possiblity that this occurs in vivo, a possible association between 125I-plasminogen (PLG) binding to the arterial wall, its activation to plasmin (PLN) and, subsequently, proteolytic damage to the intimal ECM has been studied. Intravenous injection of 125I-PLG in healthy N.Z. white male rabbits showed that PLG associated minimally (<0.01% of circulating PLG/cm2 /ml blood at 1 h) with the thoracic aorta endothelium, measured after Hautchen preparation from 1-cm vessel segments. Trans endothelial passage, measured as 125I-PLG associated with thg subendothelium (intima-media), progressed to 0.015%/cm2 /ml blood at 1 h. In contrast, the process of de-endothelialisation by balloon catheter led to a rapid uptake of bI-PLG by the denuded vessel surface. At saturation (approx. 10 min after injury), 0.7 - 0.8% of circulating PLG/cm2/ml blood was adsorbed by the entire de-endothelialised intima-media: Of the adsorbed PLG, 2-3% was associated with the platelet layer. Uptake was not inhibited by eACA (dose: 200 mg/kg) given i.v. before I-PLG. Adsorbed PLG was not released significantly from segments incubated in MEM containing 4% (w/v) RSA in vitro PLN activity was not detected. Furthermore, assessment of the ECM by transmission electron microscopy, after ruthenium red staining, showed that uptake of PLG by the de-endothelialised vessel in vivo was not associated with obvious damage to the PG components. Supported by the Heart and Stroke Foundation of Ontario.

Sign in / Sign up

Export Citation Format

Share Document