Variation in nuclear DNA content of isonicotinic acid hydrazide-resistant cell lines and mutant plants of Nicotiana tabacum

1982 ◽  
Vol 63 (1) ◽  
pp. 57-63 ◽  
Author(s):  
M. B. Berlyn
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Lines ◽  
Dna Content ◽  
Nuclear Dna ◽  
Isonicotinic Acid ◽  
Nuclear Dna Content ◽  
Acid Hydrazide ◽  
Resistant Cell ◽  
Isonicotinic Acid Hydrazide

scholarly journals Megakaryocytic cell line-specific hyperploidy by cytotoxic necrotizing factor bacterial toxins

Blood ◽  
1996 ◽  
Vol 88 (9) ◽  
pp. 3465-3473 ◽  
Author(s):  
KM Hudson ◽  
NC Denko ◽  
E Schwab ◽  
E Oswald ◽  
A Weiss ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Phorbol Ester ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Adherent Cell ◽  
Target Cells ◽  
Suspension Cells ◽  
Cytotoxic Necrotizing Factor

Cytotoxic necrotizing factor (CNF) toxins, isolated from certain Escherichia coli strains known to cause intestinal and extra intestinal infections, induce reorganization of the actin cytoskeleton and generate hyperploidy in adherent cell lines. We have examined the effect of CNF toxin on one of the few cell types that naturally increase nuclear DNA content, megakaryocytes. Our studies show that only hematopoietic cells capable of differentiating along the megakaryocyte lineage responded to the CNF2 toxin by becoming polyploid and by reorganizing actin. The K562, HEL, and CHRF-288–11 cell lines can be induced with phorbol ester to differentiate along the megakaryocyte lineage, and these cells also respond to the toxin with increased DNA content and actin cytoskeletal rearrangements. Interestingly, treatment of the K562 and HEL cell lines with CNF2 does not result in an increase in production of the megakaryocytic marker glycoprotein IIIa, unlike phorbol ester treatment. Conversely, two T-cell leukemic cell lines, CEM and Molt4, and the promyelocytic HL-60 cell line, which do not differentiate along the megakaryocyte lineage in response to phorbol myristate acetate, do not respond to CNF2, by increased expression of gpIIIa, increased nuclear DNA content, or actin reorganization. A potential target of these toxins, RhoA, is expressed by both megakaryocytic and nonmegakaryocytic cell lines, as shown by reverse transcription-polymerase chain reaction and Western blot. Although it is clear that the CNF toxins can affect a wide variety of adherent nonhematopoietic cell lines, we propose that the response to CNF, in terms of reorganizing actin structure and increase in DNA content in hematologic suspension cells, correlates with the capability of these target cells to differentiate along the megakaryocytic lineage.

scholarly journals Nuclear DNA Content and Chromatin Pattern of Rat Rhabdomyosarcoma Cell Sublines with Different Metastatic Potentials

2000 ◽  
Vol 20 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Jean Dufer ◽  
Marie-France Poupon ◽  
Sonia Yatouji
Keyword(s):  
Tumor Cells ◽  
Cell Lines ◽  
Dna Content ◽  
Nuclear Dna ◽  
Chromatin Condensation ◽  
Nuclear Dna Content ◽  
Image Cytometry ◽  
Chromatin Pattern ◽  
Rhabdomyosarcoma Cell

There is a constant need of features able to characterize potentially metastatic cells among the heterogeneous cell subpopulations which constitute a tumor. Image cytometry of metastatic tumor cells give rise to variable results, partly because of a heterogeneous origin of cells, or potential drug effects. The aim of this work was to characterize nuclear changes observed in metastatic cell clones issuedin vitrofrom the same parental cell population The nuclear phenotypes of 6 cell sublines isolated from a rat rhabdomyosarcoma cell line and differing in their metastatic ability were evaluated by image cytometry on Feulgen‐stained preparations. Densitometric [5], geometric [3] and textural [9] features were computed from each nuclear image. For each cell subline, a metastatic score, ranging from 0 to 10, was calculated on the basis ofin vitroinvasivity data, by measuring the number of pulmonary metastases observed after s.c. graft of tumor cells in rats. Data obtained were compared to karyotype, growth characteristics, and oncogene expressions of cell lines. The nuclear DNA content, the chromosome numbers, the cell sublines doubling times, and the distribution of cells within the cell cycle appear unrelated with this score. On the contrary, increase in metastatic ability is accompanied by changes in chromatin pattern as assessed by textural features. Progressive increase in chromatin condensation can be observed in cell sublines with increasing metastatic score. These results were confirmed by an unsupervised multivariate partitioning of rhabdomyosarcoma cells which identified two separate subsets whose distributions within the analyzed cell lines correlate with their metastatic ability. These data suggest that, in rat rhabdomyosarcoma cell sublines, metastatic ability could be associated with nuclear morphological changes at the level of chromatin texture.

Nuclear DNA content and chromatin texture in multidrug-resistant human leukemic cell lines

1995 ◽  
Vol 60 (1) ◽  
pp. 108-114 ◽  
Author(s):  
Jean Dufer ◽  
Christine Millot-Broguo ◽  
Zohra Oum Hamed ◽  
Francloise Lialtaud-Roger ◽  
Philippe Joly ◽  
...  
Keyword(s):  
Cell Lines ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Leukemic Cell ◽  
Multidrug Resistant ◽  
Human Leukemic Cell ◽  
Leukemic Cell Lines ◽  
Chromatin Texture

Evaluation of nuclear DNA content and heterochromatin changes in anther-derived dihaploids of tobacco (Nicotiana tabacum) cv. Coker 139

1983 ◽  
Vol 25 (2) ◽  
pp. 169-173 ◽  
Author(s):  
Sukhraj S. Dhillon ◽  
Earl A. Wernsman ◽  
Jerome P. Miksche
Keyword(s):  
Nicotiana Tabacum ◽  
Anther Culture ◽  
Dna Sequences ◽  
Dna Content ◽  
Nuclear Dna ◽  
Chromosome Doubling ◽  
Nuclear Dna Content ◽  
Contributing Factors ◽  
Root Cells ◽  
Parental Cultivar

Nuclear DNA contents of a conventionally inbred flue-cured tobacco (Nicotiana tabacum L.) cultivar. 'Coker 139,' and two selected, doubled haploid (dihaploid) lines developed from it by anther culture and colchicine chromosome doubling were compared. The dihaploid lines had previously been shown to be lower yielding and agronomically inferior to the parental cultivar. Nuclei of leaf and root cells of the dihaploids possessed significantly higher amounts of DNA than the parental cultivar, although chromosome numbers (2n = 48) had not changed. Mean nuclear DNA content of the dihaploid lines was 10.62 pg, while nuclei of the parental cultivar contained 9.32 pg. The anther-culture process and (or) the nature of vegetative nuclei of pollen grains from which haploid plants originate have been suggested as possible contributing factors to higher DNA values. An average 12% increase in heterochromatin (condensed chromatin) in dihaploids, and significant differences in thermal denaturation of parental and dihaploid DNAs, suggest an amplification of DNA sequences during dihaploid formation.

Prognostic value of nuclear DNA content in papillary and follicular thyroid cancer

1988 ◽  
Vol 12 (4) ◽  
pp. 503-507 ◽  
Author(s):  
Jaap F. Hamming ◽  
Lodewijk J. D. M. Schelfhout ◽  
Cees J. Cornelisse ◽  
Cornelis J. H. van de Velde ◽  
Bernard M. Goslings ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Dna Content ◽  
Prognostic Value ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Follicular Thyroid Cancer
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