Reproducible compartmentalization of individual chromosome domains in human CNS cells revealed by in situ hybridization and three-dimensional reconstruction

Chromosoma ◽  
1988 ◽  
Vol 96 (6) ◽  
pp. 397-410 ◽  
Author(s):  
Laura Manuelidis ◽  
Jonathan Borden
Keyword(s):  
In Situ Hybridization ◽  
Three Dimensional ◽  
Individual Chromosome ◽  
Three Dimensional Reconstruction ◽  
Chromosome Domains ◽  
Dimensional Reconstruction

scholarly journals Microcomputer-based three-dimensional reconstruction of in situ hybridization autoradiographs

1991 ◽  
Vol 4 (5) ◽  
pp. 373-385 ◽  
Author(s):  
Thomas S. Ford-Holevinski ◽  
Michael R. Castle ◽  
James P. Herman ◽  
Stanley J. Watson
Keyword(s):  
In Situ Hybridization ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction

Challenges of three-dimensional reconstruction of ribonucleoprotein complexes from electron spectroscopic images - Reconstructing ribosomal RNA

1996 ◽  
Vol 54 ◽  
pp. 52-53
Author(s):  
D.R. Beniac ◽  
G.J. Czarnota ◽  
T.A. Bartlett ◽  
F.P. Ottensmeyer ◽  
G. Harauz
Keyword(s):  
Ribosomal Rna ◽  
Correlation Functions ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Mutual Correlation ◽  
Ribosomal Subunits ◽  
E Coli ◽  
Ribonucleoprotein Complexes ◽  
Dimensional Reconstruction

Transmission electron microscopy has been dominant in structural studies of the ribosome and its constituent ribonucleic acids and proteins. Ribosomal RNA (rRNA) has central importance in the architecture of this complex and in protein synthesis. Our work has entailed using electron spectroscopic imaging (ESI) to probe the tertiary structure of rRNA in situ in a prokaryote (Escherichia coli) and in a eukaryote (Thermomyces lanuginosus). ESI uses only electrons which have lost a specific amount of energy due to specific inner-shell ionisation interactions with the specimen to form an elemental map. In nucleoprotein complexes, a map of the phosphorus distribution represents primarily a projection of the phosphate backbone of the nucleic acid component. The visualisation of rRNA in situ in the intact ribosomal subunit by ESI was demonstrated almost a decade ago to be feasible. Our work on quantitative image analysis of ES images of E. coli and Th. lanuginosus ribosomal subunits has presented unique challenges and has resulted in new algorithmic developments generally applicable to such images. These innovations include a singular pretreatment procedure, the use of mutual correlation functions rather than cross correlation functions to reduce the effect of low spatial frequency components, and angular determination using iterative quaternion-assisted angular reconstitution to compute a three-dimensional reconstruction. These investigations have produced direct information regarding ribosomal rRNA localisation in the ribosomal subunits of E. coli and Th. lanuginosus, and the position of non-conserved sequences.

Three-dimensional reconstruction of axonemal outer dynein arms in situ by electron tomography

2005 ◽  
Vol 62 (2) ◽  
pp. 69-83 ◽  
Author(s):  
Pietro Lupetti ◽  
Salvatore Lanzavecchia ◽  
David Mercati ◽  
Francesca Cantele ◽  
Romano Dallai ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction ◽  
Dynein Arms
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