Juvenile hormone stimulation of ornithine decarboxylase activity during vitellogenesis in Drosophila melanogaster

1990 ◽  
Vol 160 (2) ◽  
pp. 145-151 ◽  
Author(s):  
Mark J. Birnbaum ◽  
Lawrence I. Gilbert
Keyword(s):  
Drosophila Melanogaster ◽  
Juvenile Hormone ◽  
Ornithine Decarboxylase ◽  
Decarboxylase Activity ◽  
Hormone Stimulation ◽  
Ornithine Decarboxylase Activity ◽  
Stimulation Of

Puromycin Stimulation of Rat Liver Ornithine Decarboxylase Activity

Nature ◽  
1973 ◽  
Vol 241 (5387) ◽  
pp. 275-277 ◽  
Author(s):  
WILLIAM T. BECK ◽  
RILL ANN BELLANTONE ◽  
E. S. CANELLAKIS
Keyword(s):  
Ornithine Decarboxylase ◽  
Rat Liver ◽  
Decarboxylase Activity ◽  
Ornithine Decarboxylase Activity ◽  
Stimulation Of

scholarly journals Inhibition of ornithine decarboxylase of HeLa cells by diamines and polyamines. Effect on cell proliferation

1980 ◽  
Vol 186 (3) ◽  
pp. 925-931 ◽  
Author(s):  
Andrew A. Branca ◽  
Edward J. Herbst
Keyword(s):  
Hela Cell ◽  
Ornithine Decarboxylase ◽  
Cell Cultures ◽  
Hela Cells ◽  
Horse Serum ◽  
Fresh Medium ◽  
Decarboxylase Activity ◽  
Inhibitory Protein ◽  
Ornithine Decarboxylase Activity ◽  
Stimulation Of

1. Ornithine decarboxylase activity is stimulated in high-density HeLa-cell cultures by dilution of or replacement of spent culture medium with fresh medium containing 10% (v/v) horse serum. 2. After stimulation, ornithine decarboxylase activity reaches a peak at 4–6h, then rapidly declines to the low enzyme activity characteristic of quiescent cultures, where it remains during the remainder of the cell cycle. 3. The stimulation of ornithine decarboxylase is eliminated by the addition of 0.5μm-spermine or -spermidine or 10μm-putrescine to the HeLa-cell cultures at the time of re-feeding with fresh medium. Much higher concentrations (1mm) of the non-physiological diamines, 1,3-diamino-propane or 1,3-diamino-2-hydroxypropane, are required to eliminate the stimulation of ornithine decarboxylase in re-fed HeLa-cell cultures. 4. A heat-labile, non-diffusible inhibitor, comparable with the inhibitory protein ornithine decarboxylase antizyme, is induced in HeLa cells by the addition of exogenous diamines or polyamines. 5. Intracellular putrescine is eliminated, intracellular spermidine and spermine are severely decreased and proliferation of HeLa cells is inhibited when cultures are maintained for 48h in the presence of the non-physiological inducer of ornithine decarboxylase antizyme, 1,3-diamino-2-hydroxypropane. Exogenous putrescine, a physiological inducer of the antizyme, does not decrease intracellular polyamines or interfere with proliferation of HeLa cells.

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