Secretion of matrix metalloproteinase-2 (72 kD gelatinase/type IV collagenase = gelatinase A) by malignant human glioma cell lines: implications for the growth and cellular invasion of the extracellular matrix

1996 ◽  
Vol 28 (1) ◽  
pp. 13-24 ◽  
Author(s):  
Takao Nakagawa ◽  
Toshihiko Kubota ◽  
Masanori Kabuto ◽  
Noboru Fujimoto ◽  
Yasunori Okada
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinase ◽  
Glioma Cell ◽  
Matrix Metalloproteinase 2 ◽  
Gelatinase A ◽  
Human Glioma ◽  
Type Iv Collagenase ◽  
Cellular Invasion ◽  
Type Iv ◽  
Glioma Cell Lines

scholarly journals Calcium influx modulates expression of matrix metalloproteinase-2 (72-kDa type IV collagenase, gelatinase A).

1994 ◽  
Vol 269 (34) ◽  
pp. 21505-21511
Author(s):  
E.C. Kohn ◽  
W. Jacobs ◽  
Y.S. Kim ◽  
R. Alessandro ◽  
W.G. Stetler-Stevenson ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Calcium Influx ◽  
Matrix Metalloproteinase 2 ◽  
Gelatinase A ◽  
Type Iv Collagenase ◽  
Type Iv

scholarly journals Modulation of 72-Kilodalton Type IV Collagenase (Matrix Metalloproteinase-2) by Ascorbic Acid in Cultured Human Amnion-Derived Cells1

1998 ◽  
Vol 59 (2) ◽  
pp. 326-329 ◽  
Author(s):  
Frania Pfeffer ◽  
Esther Casanueva ◽  
Jacobo Kamar ◽  
Alejandra Guerra ◽  
Otilia Perichart ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 2 ◽  
Type Iv Collagenase ◽  
Human Amnion ◽  
Type Iv

scholarly journals Transcriptional activation by p53 of the human type IV collagenase (gelatinase A or matrix metalloproteinase 2) promoter.

1997 ◽  
Vol 17 (11) ◽  
pp. 6330-6338 ◽  
Author(s):  
J Bian ◽  
Y Sun
Keyword(s):  
Matrix Metalloproteinase ◽  
Binding Site ◽  
Matrix Metalloproteinase 2 ◽  
Dependent Manner ◽  
Wild Type ◽  
Type Iv Collagenase ◽  
Human Type ◽  
Type Iv ◽  
Wild Type P53 ◽  
Collagenase Gene

p53, a tumor suppressor and a transcription factor, has been shown to transcriptionally activate the expression of a number of important genes involved in the regulation of cell growth, DNA damage, angiogenesis, and apoptosis. In a computer search for other potential p53 target genes, we identified a perfect p53 binding site in the promoter of the human type IV collagenase (also called 72-kDa gelatinase or matrix metalloproteinase 2 [MMP-2]) gene. This p53 binding site was found to specifically bind to p53 protein in a gel shift assay. Transcription assays with luciferase reporters driven by the promoter or enhancer of the type IV collagenase gene revealed that (i) activation of the promoter activity is p53 binding site dependent in p53-positive cells but not in p53-negative cells and (ii) wild-type p53, but not p53 mutants commonly found in human cancers, transactivates luciferase expression driven by the type IV collagenase promoter as well as by a p53 site-containing enhancer element in the promoter. Significantly, expression of the endogenous type IV collagenase is also under the control of p53. Treatment of U2-OS cells, a wild-type p53-containing osteogenic sarcoma line, with a common p53 inducer, etoposide, induced p53 DNA binding and transactivation activities in a time-dependent manner. Induction of type IV collagenase expression followed the p53 activation pattern. No induction of type IV collagenase expression can be detected under the same experimental conditions in p53-negative Saos-2 cells. All these in vitro and in vivo assays strongly suggest that the type IV collagenase gene is a p53 target gene and that its expression is subject to p53 regulation. Our finding links p53 to a member of the MMP genes, a family of genes implicated in trophoblast implantation, wound healing, angiogenesis, arthritis, and tumor cell invasion. p53 may regulate these processes by upregulating expression of type IV collagenase.

Organization of F-actin filaments in human glioma cell lines cultured on extracellular matrix proteins

1996 ◽  
Vol 27 (3) ◽  
pp. 215-224 ◽  
Author(s):  
Nobuhisa Nagano ◽  
Masaru Aoyagi ◽  
Kimiyoshi Hirakawa ◽  
Mari Yamamoto ◽  
Kiyotaka Yamamoto
Keyword(s):  
Extracellular Matrix ◽  
Cell Lines ◽  
Actin Filaments ◽  
Glioma Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Human Glioma ◽  
Human Glioma Cell ◽  
Glioma Cell Lines

scholarly journals Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer

2004 ◽  
Vol 7 (4) ◽  
pp. 327-332 ◽  
Author(s):  
L Zhang ◽  
J Shi ◽  
J Feng ◽  
H Klocker ◽  
C Lee ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 2 ◽  
Type Iv Collagenase ◽  
Type Iv
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